SASDFL6

Adenine specific DNA methyltransferase (Mod Hp_0593) at pH 8

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Adenine specific DNA methyltransferase (Mod Hp_0593) at pH 8

Concentration— – 2.0 Buffer / pH25 mM Tris, 250 mM NaCl / 8.0
Experimental temperature10.0 Facility / beamlineESRF / ID14-3
Wavelength0.0992 nmExposure0.5 s × 10

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Adenine specific DNA methyltransferase (Mod)
View sequence
>TR|O25315|O25315_HELPYADENINESPECIFICDNAMETHYLTRANSFERASE(MOD)OS=HELICOBACTERPYLORI(STRAINATCC700392/26695)OX=85962GN=HP_0593PE=4SV=1MLLKNFPQTIKDGQVSLSAIKMLLGFNESMNDISGYELTWTGKGFANALYSEPCQKQLKLQESFTPQTSASKHPNNAIIIGDNLDALKLLKSAYSEKIKMIYIDPPYNTGNDEFIYPDNFRQDYQKILREVGLMEIDENGKEIESESLKFFKNTQGSGTHSGWLSFMLPRLKLARDLLKEDGVIFISIDDNECANLKILCDEIFGEDNFVGDFIRKTKSTTNDAKIGLNYQHEFLLCYAKDKNYTNLLGGEKNLENYKNPDNDPNGAWINDNPSAKSGNMKTGYFGVTNPYTNKVDYPPVGMFWRFSQNTIQKHIDEGRICFKKEHKDNERGFIYKRYLKDLKTTQKTFDSLIFSDNCYMNQAATKELLNLGMGEYFTYPKGVEFMKKIILHSTTPNEGDIILDFFAGSGTTVHAVMELNAEDKGNREFILVQIDEEIKEDESAYDFCKKELKSAKPVISDITIERVKRAAQKISQLSKDSGLDLGFKVYTLQDKVQIINDKEEITLFNRSDLTPFDKALNLALQCGKTLNQALEIIIKDKLYKCEDAYFCIVCDEEAQEYLAKSKNEMIFLDGYEEIDLEAFLNLNASFKERLSVVY
proteinHelicobacter pyloriO253151–598dimerMolecule count 268.3 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11036[3]0.03553313–4.939105 1/nmHas uncertainty columnMissing 039sasbdb/entries/l6/sasdfl6/source/SASDFL6.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)12.5—nm
i0Guinier35.570.086arbitrary
i0P(r)35.98—arbitrary
mwExperimental120.0—kDa
mwPorod90.0—kDa
porod_volumePorod143.0—nm³
rgGuinier3.270.74nm
rgP(r)3.375—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25 mM Tris, 250 mM NaClBuffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2017-07-09Storage / measurement temperature10.0 / 10.0
Exposure time0.5Frames10
Wavelength0.0992Sample-to-detector distance—
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineESRF / ID14-3 · Grenoble, France
q range0.036 – 4.939Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:179 field values
Field pathRaw value
codeSASDFL6
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleTetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.
project.publication.titleTetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.
project.publication.author_listNarayanan N, Banerjee A, Jain D, Kulkarni DS, Sharma R, Nirwal S, Rao DN, Nair DT
project.publication.journalJ Mol Biol
project.publication.doi10.1016/j.jmb.2019.10.001
project.publication.pmid31628946
project.publication.published_date2019 Oct 16
project.statusreleased
project.submitted_date2019-05-31
project.released_date2019-11-17
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameID14-3
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameAdenine specific DNA methyltransferase (Mod)
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequence>tr|O25315|O25315_HELPY Adenine specific DNA methyltransferase (Mod) OS=Helicobacter pylori (strain ATCC 700392 / 26695) OX=85962 GN=HP_0593 PE=4 SV=1 MLLKNFPQTIKDGQVSLSAIKMLLGFNESMNDISGYELTWTGKGFANALYSEPCQKQLKL QESFTPQTSASKHPNNAIIIGDNLDALKLLKSAYSEKIKMIYIDPPYNTGNDEFIYPDNF RQDYQKILREVGLMEIDENGKEIESESLKFFKNTQGSGTHSGWLSFMLPRLKLARDLLKE DGVIFISIDDNECANLKILCDEIFGEDNFVGDFIRKTKSTTNDAKIGLNYQHEFLLCYAK DKNYTNLLGGEKNLENYKNPDNDPNGAWINDNPSAKSGNMKTGYFGVTNPYTNKVDYPPV GMFWRFSQNTIQKHIDEGRICFKKEHKDNERGFIYKRYLKDLKTTQKTFDSLIFSDNCYM NQAATKELLNLGMGEYFTYPKGVEFMKKIILHSTTPNEGDIILDFFAGSGTTVHAVMELN AEDKGNREFILVQIDEEIKEDESAYDFCKKELKSAKPVISDITIERVKRAAQKISQLSKD SGLDLGFKVYTLQDKVQIINDKEEITLFNRSDLTPFDKALNLALQCGKTLNQALEIIIKD KLYKCEDAYFCIVCDEEAQEYLAKSKNEMIFLDGYEEIDLEAFLNLNASFKERLSVVY
experiment.sample.molecule[0].organismHelicobacter pylori
experiment.sample.molecule[0].uniprot_codeO25315
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last598
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMLLKNFPQTIKDGQVSLSAIKMLLGFNESMNDISGYELTWTGKGFANALYSEPCQKQLKL QESFTPQTSASKHPNNAIIIGDNLDALKLLKSAYSEKIKMIYIDPPYNTGNDEFIYPDNF RQDYQKILREVGLMEIDENGKEIESESLKFFKNTQGSGTHSGWLSFMLPRLKLARDLLKE DGVIFISIDDNECANLKILCDEIFGEDNFVGDFIRKTKSTTNDAKIGLNYQHEFLLCYAK DKNYTNLLGGEKNLENYKNPDNDPNGAWINDNPSAKSGNMKTGYFGVTNPYTNKVDYPPV GMFWRFSQNTIQKHIDEGRICFKKEHKDNERGFIYKRYLKDLKTTQKTFDSLIFSDNCYM NQAATKELLNLGMGEYFTYPKGVEFMKKIILHSTTPNEGDIILDFFAGSGTTVHAVMELN AEDKGNREFILVQIDEEIKEDESAYDFCKKELKSAKPVISDITIERVKRAAQKISQLSKD SGLDLGFKVYTLQDKVQIINDKEEITLFNRSDLTPFDKALNLALQCGKTLNQALEIIIKD KLYKCEDAYFCIVCDEEAQEYLAKSKNEMIFLDGYEEIDLEAFLNLNASFKERLSVVY
experiment.sample.molecule[0].mw68.3
experiment.sample.molecule[0].total_mw136.6
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name25 mM Tris, 250 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameAdenine specific DNA methyltransferase (Mod Hp_0593) at pH 8
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameRCB
experiment.contributor[0].affiliation[0].addressHaryana, India
experiment.contributor[0].affiliation[0].full_nameRegional Centre for Biotechnology
experiment.contributor[0].affiliation[0].webpagehttp://www.rcb.res.in/
experiment.contributor[0].contributor_nameNaveen
experiment.contributor[0].contributor_surnameNarayanan
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-8327-1559
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-07-09
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time0.5
experiment.number_of_frames10
experiment.wavelength0.0992
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_max2.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.036
experiment.s_max4.939
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].p_value0.0
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fits[1].p_value0.0
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estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of adenine specific DNA methyltransferase (Mod Hp_0593) at pH 8 in 25 mM Tris, 250 mM NaCl were collected on the ID14-3 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw120.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw90.0
porod_mw_errornull
pddf_i035.98
pddf_i0_errornull
guinier_i035.57
guinier_i0_error0.086
pddf_rg3.375
pddf_rg_errornull
guinier_rg3.27
guinier_rg_error0.74
pddf_dmax12.5
pddf_dmax_errornull
porod_volume143.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first30
guinier_point_last77
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDFL6_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDFL6
statussuccess
started_at2026-08-11T15:11:52.764339+00:00
finished_at2026-08-11T15:12:04.033331+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDFL6",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.",
    "publication": {
      "title": "Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.",
      "author_list": "Narayanan N, Banerjee A, Jain D, Kulkarni DS, Sharma R, Nirwal S, Rao DN, Nair DT",
      "journal": "J Mol Biol",
      "doi": "10.1016/j.jmb.2019.10.001",
      "pmid": "31628946",
      "published_date": "2019 Oct 16"
    },
    "status": "released",
    "submitted_date": "2019-05-31",
    "released_date": "2019-11-17"
  },
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  "experiment": {
    "instrument": {
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View the complete manifest.json source
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