Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 305-309)

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  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.",
    "publication": {
      "title": "Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.",
      "author_list": "Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B",
      "journal": "J Struct Biol",
      "doi": "10.1016/j.jsb.2019.107429",
      "pmid": "31778770",
      "published_date": "2019 Nov 25"
    },
    "status": "released",
    "submitted_date": "2019-01-30",
    "released_date": "2020-01-09"
  },
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFX2.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Rayonix MX225-HE",
        "resolution": null
      },
      "name": "Stanford Synchrotron Radiation Lightsource (SSRL)",
      "city": "Menlo Park, CA",
      "country": "USA",
      "beamline_name": "BL4-2",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "Cell wall synthesis protein Wag31",
          "short_name": null,
          "sequence": "MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL\r\nAAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT\r\nAKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA\r\nDALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA\r\nAPVDSNADAGGFDQFNRGKN",
          "organism": "Mycobacterium tuberculosis",
          "uniprot_code": "P9WMU1",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "unknown",
          "molecular_type": "protein",
          "uniprot_sequence": "MPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL\nAAGGGAGVTPQATQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT\nAKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA\nDALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA\nAPVDSNADAGGFDQFNRGKN",
          "mw": 29.98,
          "total_mw": 29.98,
          "number_molecules": 0,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 305-309)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "CSIR",
            "address": "Chandigarh, India",
            "full_name": "CSIR-Institute of Microbial Technology",
            "webpage": "http://www.imtech.res.in/"
          }
        ],
        "contributor_name": "Komal",
        "contributor_surname": "Choukate",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2018-01-29",
    "storage_temperature": 23.0,
    "cell_temperature": 23.0,
    "exposure_time": 1.0,
    "number_of_frames": 500,
    "wavelength": 0.1,
    "sample_detector_distance": 2.54,
    "concentration_min": null,
    "concentration_max": 2.4,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.055,
    "s_max": 4.023,
    "total_exposure_time": null,
    "seccolumn": null
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  "description": "SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak.\r\nSEC-SAXS data of Cell wall synthesis protein Wag31(T73A) polymer frame no. 305-309",
  "experiment_description": "Synchrotron SAXS data from solutions of Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 305-309) in 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH 7.5 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring (Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.40 mg/ml was measured at 23°C. 500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
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  "estimated_volume_error": null,
  "guinier_point_first": 5,
  "guinier_point_last": 15,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
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  "symmetry": null,
  "last_modified": "2020-08-13T15:34:35.760459+02:00",
  "bragg_peak": []
}

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