SASDFZ4

R16-24 human dystrophin fragment

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2023-06-20T14:43:31.013409+02:00

SEC-SAXS was performed at 20°C using the following parameters: Column: BioSEC5-500Å (4.6 mm id * 300 mm); Flow rate: 0.3 mL/min; Sample injection concentration: 4.5 mg/mL; Injection volume: 50μL. The data were collected through the SEC peak of the protein as a series of 38 x 0.75 second exposures. The experimental molecular weight was determined from SEC-MALS (130 kDa).

▼

1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · R16-24 human dystrophin fragment

Concentration	— – —	Buffer / pH	NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2% / 7.5
Experimental temperature	20.0	Facility / beamline	SOLEIL / SWING
Wavelength	0.1033 nm	Exposure	0.75 s × 51

Molecular Components

Component	Type / organism	UniProt and Construct	Oligomeric State	Molecular weight
Human dystrophin central domain R16-24 fragment View sequence MSYYHHHHHHDYDIPTTENLYFOGAMGRSLVPRGSLEISYVPSTYLTEITHVSQALLEVEQLLNAPDLCAKDFEDLFKQEESLKNIKDSLQQSSGRIDIIHSKKTAALQSATPVERVKLQEALSQLDFQWEKVNKMYKDRQGRFDRSVEKWRRFHYDIKIFNQWLTEAEQFLRKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRTLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLSDRKKRLEEQKNILSEFQRDLNEFVLWLEEADNIASIPLEPGKEQQLKEKLEQVKLLVEELPLRQAGILKQLNETGGPVLVSAPISPEEQDKLENKLKQTNLQWIKVSRALPEKQGEIEAQIKDLGQLEKKLEDLEEQLNHLLLWLSPIRNQLEIYNQPNQEGPFDVQETEIAVQAKQPDVEEILSKGQHLYKEKPATQPVKRKLEDLSSEWKAVNRLLQELRAKQPDLAPGLTTIGASPTQTVTLVTQPVVTKETAISKLEMPSSLMLEVPALADFNRAWTELTDWLSLLDQVIKSQRVMVGDLEDINEMIIKQKATMQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQNQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEGPYTVDAIQKKITETKQLAKDLRQWQTNVDVANDLALKLLRDYSADDTRKVHMITENINASWRSIHKRVSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATRKERLLEDSKGVKELMKQWQDLQGEIEAHTDVYHNLDENSQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDETLERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVNDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRVRQLHE	protein—	P115321990–3040	monomerMolecule count 1	126.616 kDa

Experimental Curves

Curve	Points / columns	q range	Uncertainty quality	Negative-intensity points	Source file
1	1171[3]	0.00405745143037–0.595310672418 1/A	Has uncertainty columnMissing 0	219	sasbdb/entries/z4/sasdfz4/source/SASDFZ4.dat

▼

2. Measurements Reported by SASBDB Reported Results

Metric	Method	Value	Uncertainty	Unit
dmax	P(r)	34.0	—	Å
i0	Guinier	1.05	—	arbitrary
i0	P(r)	1.037	—	arbitrary
mw	Experimental	130.0	—	kDa
rg	Guinier	9.28	—	Å
rg	P(r)	9.38	—	Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

▼

3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

▶

4. Source File Index Source Files

Category	Path	Download status	Size
curve	sasbdb/entries/z4/sasdfz4/source/SASDFZ4.dat	downloaded	64624
full_entry_zip	sasbdb/entries/z4/sasdfz4/source/SASDFZ4.zip	downloaded	35977
pddf	sasbdb/entries/z4/sasdfz4/source/SASDFZ4.out	downloaded	36645
sascif	sasbdb/entries/z4/sasdfz4/source/SASDFZ4.sascif	downloaded	94694
summary	sasbdb/entries/z4/sasdfz4/source/summary.json	downloaded	10629

▶

5. Experiment Description and Publication Experiment & Publication

▼

6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name	NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%	Buffer concentration	—
pH	7.5	Additives	—
Buffer description	—
Purity measurement method	—	Extinction coefficient	—
Absorbance	—	Scattering contrast	—
Specific volume / dry volume	— / —	Mixture / deuteration	— / —

Acquisition Conditions and Instrument

Measurement date	2016-12-17	Storage / measurement temperature	— / 20.0
Exposure time	0.75	Frames	51
Wavelength	0.1033	Sample-to-detector distance	2.0
Source	X-ray synchrotron	Detector	AVIEX PCCD170170
Facility / beamline	SOLEIL / SWING · Saint-Aubin, France
q range	0.041 – 5.953	Sample volume / flow rate	— / —

Original SASBDB Plots

Downloadable Files

Category	File	Status	Size	Checksum	Download and View
curve	sasbdb/entries/z4/sasdfz4/source/SASDFZ4.dat	downloaded	64624	e2be6608e66b6fcb68335c04877df501be7879f3d428bf9015caccf3992fd77c	DownloadView source fileSource site
full_entry_zip	sasbdb/entries/z4/sasdfz4/source/SASDFZ4.zip	downloaded	35977	f71f94095350497cf5bd99c1e22d6af7eef0020271a302f39927c9414e19119e	DownloadView source fileSource site
pddf	sasbdb/entries/z4/sasdfz4/source/SASDFZ4.out	downloaded	36645	272296f895b514f35049d0abe9edaadc2de804f17dacfaf5dec1261e2992731c	DownloadView source fileSource site
sascif	sasbdb/entries/z4/sasdfz4/source/SASDFZ4.sascif	downloaded	94694	561c3f3ae0c3bd2c5f282225aecefcee0bc2f27067fee1bbb8c1bc873d38e17f	DownloadView source fileSource site
summary	sasbdb/entries/z4/sasdfz4/source/summary.json	downloaded	10629	d12b4b874dd8ab5bf31c09e50bde05d178c63152733a4aa1cede093fd651cbf5	DownloadView source fileSource site

curve：source record

{
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  "role": "curve",
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  "size": 64624,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/intensities_files/SASDFZ4.dat"
}

full_entry_zip：source record and ZIP contents (2 items)

{
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  "role": "full_entry_zip",
  "sha256": "f71f94095350497cf5bd99c1e22d6af7eef0020271a302f39927c9414e19119e",
  "size": 35977,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/zip_directories/SASDFZ4.zip",
  "zip_member_count": 2,
  "zip_members": [
    {
      "crc32": "5f0227c1",
      "name": "SASDFZ4/experimental_data/SASDFZ4.dat",
      "size": 64624
    },
    {
      "crc32": "dd505276",
      "name": "SASDFZ4/pddf/SASDFZ4.out",
      "size": 36645
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  ],
  "zip_validation": "central_directory_readable"
}

pddf：source record

{
  "path": "/data/saxsdb/sasbdb/entries/z4/sasdfz4/source/SASDFZ4.out",
  "role": "pddf",
  "sha256": "272296f895b514f35049d0abe9edaadc2de804f17dacfaf5dec1261e2992731c",
  "size": 36645,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/p_of_R_files/SASDFZ4.out"
}

sascif：source record

{
  "path": "/data/saxsdb/sasbdb/entries/z4/sasdfz4/source/SASDFZ4.sascif",
  "role": "sascif",
  "sha256": "561c3f3ae0c3bd2c5f282225aecefcee0bc2f27067fee1bbb8c1bc873d38e17f",
  "size": 94694,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFZ4.sascif"
}

summary：source record

{
  "path": "/data/saxsdb/sasbdb/entries/z4/sasdfz4/source/summary.json",
  "sha256": "d12b4b874dd8ab5bf31c09e50bde05d178c63152733a4aa1cede093fd651cbf5",
  "size": 10629,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDFZ4/"
}

All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json：129 field values

Field path	Raw value
code	SASDFZ4
status	Published
type_of_curve	Single concentration
angular_unit	1/A
project.title	Dystrophin SAXS data
project.publication	null
project.status	released
project.submitted_date	2019-03-30
project.released_date	2023-03-30
pddf_data	https://www.sasbdb.org/media/p_of_R_files/SASDFZ4.out
intensities_data	https://www.sasbdb.org/media/intensities_files/SASDFZ4.dat
intensities_log_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFZ4_dat_img.png
intensities_kratky_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFZ4_kratky_img.png
pddf_plot	https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFZ4_pofr_img.png
intensities_guinier_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFZ4_guinier_img.png
sascif_data	https://www.sasbdb.org/media/sascif/sascif_files/SASDFZ4.sascif
experiment.instrument.detector.type	CCD
experiment.instrument.detector.name	AVIEX PCCD170170
experiment.instrument.detector.resolution	null
experiment.instrument.name	SOLEIL
experiment.instrument.city	Saint-Aubin
experiment.instrument.country	France
experiment.instrument.beamline_name	SWING
experiment.instrument.beam_geometry
experiment.instrument.type_of_source	X-ray synchrotron
experiment.instrument.point_source	null
experiment.instrument.line_collimation	null
experiment.instrument.sample_path_length	null
experiment.instrument.line_collimation_slitlength	null
experiment.instrument.line_collimation_integrationwidth	null
experiment.instrument.xray_energy	null
experiment.instrument.beam_profile_ah	null
experiment.instrument.beam_profile_al	null
experiment.sample.molecule[0].long_name	Human dystrophin central domain R16-24 fragment
experiment.sample.molecule[0].short_name	null
experiment.sample.molecule[0].sequence	MSYYHHHHHHDYDIPTTENLYFOGAMGRSLVPRGSLEISYVPSTYLTEITHVSQALLEVEQLLNAPDLCAKDFEDLFKQEESLKNIKDSLQQSSGRIDIIHSKKTAALQSATPVERVKLQEALSQLDFQWEKVNKMYKDRQGRFDRSVEKWRRFHYDIKIFNQWLTEAEQFLRKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRTLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLSDRKKRLEEQKNILSEFQRDLNEFVLWLEEADNIASIPLEPGKEQQLKEKLEQVKLLVEELPLRQAGILKQLNETGGPVLVSAPISPEEQDKLENKLKQTNLQWIKVSRALPEKQGEIEAQIKDLGQLEKKLEDLEEQLNHLLLWLSPIRNQLEIYNQPNQEGPFDVQETEIAVQAKQPDVEEILSKGQHLYKEKPATQPVKRKLEDLSSEWKAVNRLLQELRAKQPDLAPGLTTIGASPTQTVTLVTQPVVTKETAISKLEMPSSLMLEVPALADFNRAWTELTDWLSLLDQVIKSQRVMVGDLEDINEMIIKQKATMQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQNQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEGPYTVDAIQKKITETKQLAKDLRQWQTNVDVANDLALKLLRDYSADDTRKVHMITENINASWRSIHKRVSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATRKERLLEDSKGVKELMKQWQDLQGEIEAHTDVYHNLDENSQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDETLERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVNDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRVRQLHE
experiment.sample.molecule[0].organism	null
experiment.sample.molecule[0].uniprot_code	P11532
experiment.sample.molecule[0].uniprot_range_first	1990
experiment.sample.molecule[0].uniprot_range_last	3040
experiment.sample.molecule[0].oligomerization	monomer
experiment.sample.molecule[0].molecular_type	protein
experiment.sample.molecule[0].uniprot_sequence	MLWWEEVEDCYEREDVQKKTFTKWVNAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQ KLPKEKGSTRVHALNNVNKALRVLQNNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQV KNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDL FDWNSVVCQQSATQRLEHAFNIARYQLGIEKLLDPEDVDTTYPDKKSILMYITSLFQVLP QQVSIEAIQEVEMLPRPPKVTKEEHFQLHHQMHYSQQITVSLAQGYERTSSPKPRFKSYA YTQAAYVTTSDPTRSPFPSQHLEAPEDKSFGSSLMESEVNLDRYQTALEEVLSWLLSAED TLQAQGEISNDVEVVKDQFHTHEGYMMDLTAHQGRVGNILQLGSKLIGTGKLSEDEETEV QEQMNLLNSRWECLRVASMEKQSNLHRVLMDLQNQKLKELNDWLTKTEERTRKMEEEPLG PDLEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRW ANICRWTEDRWVLLQDILLKWQRLTEEQCLFSAWLSEKEDAVNKIHTTGFKDQNEMLSSL QKLAVLKADLEKKKQSMGKLYSLKQDLLSTLKNKSVTQKTEAWLDNFARCWDNLVQKLEK STAQISQAVTTTQPSLTQTTVMETVTTVTTREQILVKHAQEELPPPPPQKKRQITVDSEI RKRLDVDITELHSWITRSEAVLQSPEFAIFRKEGNFSDLKEKVNAIEREKAEKFRKLQDA SRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIIAFYNQLQ QLEQMTTTAENWLKIQPTTPSEPTAIKSQLKICKDEVNRLSDLQPQIERLKIQSIALKEK GQGPMFLDADFVAFTNHFKQVFSDVQAREKELQTIFDTLPPMRYQETMSAIRTWVQQSET KLSIPQLSVTDYEIMEQRLGELQALQSSLQEQQSGLYYLSTTVKEMSKKAPSEISRKYQS EFEEIEGRWKKLSSQLVEHCQKLEEQMNKLRKIQNHIQTLKKWMAEVDVFLKEEWPALGD SEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKNEAEPEFASRLETELKELNTQWDH MCQQVYARKEALKGGLEKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQKAVEEM KRAKEEAQQKEAKVKLLTESVNSVIAQAPPVAQEALKKELETLTTNYQWLCTRLNGKCKT LEEVWACWHELLSYLEKANKWLNEVEFKLKTTENIPGGAEEISEVLDSLENLMRHSEDNP NQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQETEKSLH LIQESLTFIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKEAAQRV LSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQS QLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVT ERKQQLEKCLKLSRKMRKEMNVLTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKE IEKQKVHLKSITEVGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKH METFDQNVDHITKWIIQADTLLDESEKKKPQQKEDVLKRLKAELNDIRPKVDSTRDQAAN LMANRGDHCRKLVEPQISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAE IQQGVNLKEEDFNKDMNEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNA LKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQ KKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVREE TMMVMTEDMPLEISYVPSTYLTEITHVSQALLEVEQLLNAPDLCAKDFEDLFKQEESLKN IKDSLQQSSGRIDIIHSKKTAALQSATPVERVKLQEALSQLDFQWEKVNKMYKDRQGRFD RSVEKWRRFHYDIKIFNQWLTEAEQFLRKTQIPENWEHAKYKWYLKELQDGIGQRQTVVR TLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLSDRKKRLEEQKNILSEFQRDL NEFVLWLEEADNIASIPLEPGKEQQLKEKLEQVKLLVEELPLRQGILKQLNETGGPVLVS APISPEEQDKLENKLKQTNLQWIKVSRALPEKQGEIEAQIKDLGQLEKKLEDLEEQLNHL LLWLSPIRNQLEIYNQPNQEGPFDVKETEIAVQAKQPDVEEILSKGQHLYKEKPATQPVK RKLEDLSSEWKAVNRLLQELRAKQPDLAPGLTTIGASPTQTVTLVTQPVVTKETAISKLE MPSSLMLEVPALADFNRAWTELTDWLSLLDQVIKSQRVMVGDLEDINEMIIKQKATMQDL EQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQNQWDEVQEHLQNRRQQLNEMLK DSTQWLEAKEEAEQVLGQARAKLESWKEGPYTVDAIQKKITETKQLAKDLRQWQTNVDVA NDLALKLLRDYSADDTRKVHMITENINASWRSIHKRVSEREAALEETHRLLQQFPLDLEK FLAWLTEAETTANVLQDATRKERLLEDSKGVKELMKQWQDLQGEIEAHTDVYHNLDENSQ KILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVW LQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEG LEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDETLERLQELQ EATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVNDLAR QLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRVRQLHEAHRDFGPASQHFLSTSVQGP WERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALC LDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLN WLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHD SIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHR VAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYC TPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDS APASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLN QDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLP SPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQP QAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSDSMGEEDLLSPPQDTSTGLEEVM EQLNNSFPSSRGRNTPGKPMREDTM
experiment.sample.molecule[0].mw	126.616
experiment.sample.molecule[0].total_mw	126.616
experiment.sample.molecule[0].number_molecules	1
experiment.sample.molecule[0].complex_state	False
experiment.sample.molecule[0].deuteration	null
experiment.sample.molecule[0].molecule_source	biological
experiment.sample.molecule[0].molecule_description	null
experiment.sample.buffer.name	NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%
experiment.sample.buffer.concentration_unit	null
experiment.sample.buffer.comment	null
experiment.sample.buffer.additive	null
experiment.sample.buffer.concentration	null
experiment.sample.buffer.pka	null
experiment.sample.buffer.ph	7.5
experiment.sample.buffer.deuteration	null
experiment.sample.purity_method	null
experiment.sample.name	R16-24 human dystrophin fragment
experiment.sample.ext_coefficient	null
experiment.sample.contrast	null
experiment.sample.specific_vol	null
experiment.sample.dry_vol	null
experiment.sample.absorbption	null
experiment.sample.deuteration	null
experiment.sample.mixture	null
experiment.contributor[0].affiliation[0].short_name	null
experiment.contributor[0].affiliation[0].address	France
experiment.contributor[0].affiliation[0].full_name	University of Lorraine
experiment.contributor[0].affiliation[0].webpage	http://welcome.univ-lorraine.fr/
experiment.contributor[0].contributor_name	Raphael
experiment.contributor[0].contributor_surname	Dos Santos Morais
experiment.contributor[0].orcid
experiment.concentration_method	null
experiment.concentration_unit	null
experiment.date	2016-12-17
experiment.storage_temperature	null
experiment.cell_temperature	20.0
experiment.exposure_time	0.75
experiment.number_of_frames	51
experiment.wavelength	0.1033
experiment.sample_detector_distance	2.0
experiment.concentration_min	null
experiment.concentration_max	null
experiment.sample_volume	null
experiment.flow_rate	null
experiment.s_min	0.041
experiment.s_max	5.953
experiment.total_exposure_time	null
experiment.seccolumn	null
fits	[]
estimated_volume_method	null
pddf_software	ATSAS GNOM
pddf_software_version	4.6
i0_calibration_standard	null
description	SEC-SAXS was performed at 20°C using the following parameters: Column: BioSEC5-500Å (4.6 mm id * 300 mm); Flow rate: 0.3 mL/min; Sample injection concentration: 4.5 mg/mL; Injection volume: 50μL. The data were collected through the SEC peak of the protein as a series of 38 x 0.75 second exposures. The experimental molecular weight was determined from SEC-MALS (130 kDa).
experiment_description	Synchrotron SAXS data from solutions of the R16-24 human dystrophin fragment in NaP 20 mM, NaCl 300 mM, EDTA 1 mM, glycerol 2%, pH 7.5 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the SWING beam line SOLEIL (Saint-Aubin, France) using a CCD AVIEX PCCD170170 detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1033 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags	[]
intensity_unit	arbitrary
experimental_mw	130.0
experimental_mw_error	null
guinier_i0_mw	null
guinier_i0_mw_error	null
porod_mw	null
porod_mw_error	null
pddf_i0	1.037
pddf_i0_error	null
guinier_i0	1.05
guinier_i0_error	null
pddf_rg	9.38
pddf_rg_error	null
guinier_rg	9.28
guinier_rg_error	null
pddf_dmax	34.0
pddf_dmax_error	null
porod_volume	null
porod_volume_error	null
estimated_volume	null
estimated_volume_error	null
guinier_point_first	null
guinier_point_last	null
pddf_point_first	null
pddf_point_last	null
i0_calibration_standard_data	null
intensities_log_log_plot	SASDFZ4_datloglog_img.png
symmetry	null
last_modified	2023-06-20T14:43:31.013409+02:00
bragg_peak	[]

manifest.json：36 field values

Field path	Raw value
code	SASDFZ4
status	success
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View the complete summary.json source

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  "status": "Published",
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    "title": "Dystrophin SAXS data",
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