查看完整 summary.json 原文
{
"code": "SASDUW7",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/A",
"project": {
"title": "The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures",
"publication": {
"title": "The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures",
"author_list": "Bradshaw W, Harris G, Gileadi O, Katis V",
"journal": "Structure",
"doi": "10.1016/j.str.2024.09.024",
"pmid": null,
"published_date": "2024 Oct"
},
"status": "released",
"submitted_date": "2024-04-29",
"released_date": "2024-10-07"
},
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"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDUW7_pofr_img.png",
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"experiment": {
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"name": "Eiger 4M",
"resolution": 75.0
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"name": "Diamond Light Source",
"city": "Didcot",
"country": "UK",
"beamline_name": "B21",
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"type_of_source": "X-ray synchrotron",
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"sample": {
"molecule": [
{
"long_name": "Tyrosine-protein kinase SYK",
"short_name": "SYK",
"sequence": "SMADSANHLPFFFGNITREEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRKAHHYTIERELNGTYAIAGGRTHASPADLCHYHSQESDGLVCLLKKPFNRPQGVQPKTGPFEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLIATTAHEKMPWFHGKISREESEQIVLIGSKTNGKFLIRARDNNGSYALCLLHEGKVLHYRIDKDKTGKLSIPEGKKFDTLWQLVEHYSYKADGLLRVLTVPCQKIGTQGNVN",
"organism": "Homo sapiens",
"uniprot_code": "P43405",
"uniprot_range_first": 6,
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"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MASSGMADSANHLPFFFGNITREEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRK\nAHHYTIERELNGTYAIAGGRTHASPADLCHYHSQESDGLVCLLKKPFNRPQGVQPKTGPF\nEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLIATTAHEKMPWFHGKISREESEQ\nIVLIGSKTNGKFLIRARDNNGSYALCLLHEGKVLHYRIDKDKTGKLSIPEGKKFDTLWQL\nVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPK\nPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRP\nKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAE\nANVMQQLDNPYIVRMIGICEAESWMLVMEMAELGPLNKYLQQNRHVKDKNIIELVHQVSM\nGMKYLEESNFVHRDLAARNVLLVTQHYAKISDFGLSKALRADENYYKAQTHGKWPVKWYA\nPECINYYKFSSKSDVWSFGVLMWEAFSYGQKPYRGMKGSEVTAMLEKGERMGCPAGCPRE\nMYDLMNLCWTYDVENRPGFAAVELRLRNYYYDVVN",
"mw": 29.862,
"total_mw": 29.862,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": ""
},
{
"long_name": "TYRO protein tyrosine kinase-binding protein",
"short_name": "TYROBP",
"sequence": "ESPYQELQGQRSDVYSDLNT",
"organism": "Homo sapiens",
"uniprot_code": "O43914",
"uniprot_range_first": 88,
"uniprot_range_last": 107,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MGGLEPCSRLLLLPLLLAVSGLRPVQAQAQSDCSCSTVSPGVLAGIVMGDLVLTVLIALA\nVYFLGRLVPRGRGAAEAATRKQRITETESPYQELQGQRSDVYSDLNTQRPYYK",
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"deuteration": null,
"molecule_source": "biological",
"molecule_description": ""
}
],
"buffer": {
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"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 7.5,
"deuteration": null
},
"purity_method": null,
"name": "The tandem SH2 domains of Tyrosine-protein kinase SYK with a bound TYROBP diphospho-ITAM peptide",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Oxford, UK",
"full_name": "University of Oxford",
"webpage": "http://www.ox.ac.uk/"
}
],
"contributor_name": "William",
"contributor_surname": "Bradshaw",
"orcid": "https://orcid.org/0000-0002-7985-9752"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2023-11-13",
"storage_temperature": 15.0,
"cell_temperature": 15.0,
"exposure_time": 3.0,
"number_of_frames": 600,
"wavelength": 0.09408,
"sample_detector_distance": 3.712,
"concentration_min": null,
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"sample_volume": 45.0,
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"seccolumn": 4
},
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"pddf_software": "ScÅtter",
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"description": null,
"experiment_description": "Synchrotron SAXS data from solutions of the tandem SH2 domains of Tyrosine-protein kinase SYK with a bound TYROBP diphospho-ITAM peptide in 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.09408 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 10 mg/ml was injected at a 0.08 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 15°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
"intensity_unit": "1/cm",
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"guinier_i0_mw": null,
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"pddf_dmax": 7.05,
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"porod_volume": 52.51,
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"estimated_volume": null,
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"guinier_point_first": 1,
"guinier_point_last": 362,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDUW7_datloglog_img.png",
"symmetry": null,
"last_modified": "2024-10-23T10:59:49.720798+02:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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