SASDUZ5

Extrapolated scattering curve of dimeric Kluyveromyces lactis glucokinase 1 (KlGlk1)

Data type: SASBDB experimental data Status: Published Curve type: Extrapolated to infinite dilution Last updated: 2026-03-27T11:00:12.571151+01:00

Extropolation to infinite dilution using ALMERGE 1.1 (r11734) with scattering data of KlGlk1 with the following concentrations: 14.2 mg/mL, 7.3 mg/mL, 3.7 mg/mL, 1.9 mg/mL

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Extrapolated scattering curve of dimeric Kluyveromyces lactis glucokinase 1 (KlGlk1)

Concentration14.2 – 1.9 Buffer / pH10 mM Tris-HCl, 1 mM DTT / 7.4
Experimental temperature10.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.12399 nmExposure0.045 s × 30

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Glucokinase-1
View sequence
MSDPKLTKAVDSICDQFIVTKSKISQLTEYFIDCMEKGLEPCESDISQNKGLPMIPTFVTDKPSGQEHGVTMLAADLGGTNFRVCSVELLGNHEFKIEQEKSKIPTFFFQDDHHVTSKDLFQHMALITHQFLTKHHKDVIQDYKWKMGFTFSYPVDQTSLSSGKLIRWTKGFKIGDTVGQDVVQLFQQELNDIGLSNVHVVALTNDTTGTLLARCYASSDAARAINEPVIGCIFGTGTNGCYMEKLENIHKLDPASREELLSQGKTHMCINTEWGSFDNELNHLPTTSYDIKIDQQFSTNPGFHLFEKRVSGLYLGEILRNILLDLEKQELFDLKESVLKNNPFILTTETLSHIEIDTVENDLQDTRDALLKAADLETTFEERVLIQKLVRAISRRAAFLAAVPIAAILIKTNALNQSYHCQVEVGCDGSVVEHYPGFRSMMRHALALSPIGPEGERDVHLRISKDGSGVGAALCALHANY
proteinKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)Q6CUZ31–481dimerMolecule count 253.757 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11812[3]0.0304874–4.81767 1/nmHas uncertainty columnMissing 03sasbdb/entries/z5/sasduz5/source/SASDUZ5.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)9.94—nm
i0Guinier0.0660649—1/cm
i0P(r)0.06502—1/cm
mwExperimental94.225—kDa
mwPorod96.0—kDa
porod_volumePorod154.0—nm³
rgGuinier3.3640.003nm
rgP(r)3.289—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name10 mM Tris-HCl, 1 mM DTTBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2015-10-20Storage / measurement temperature10.0 / 10.0
Exposure time0.045Frames30
Wavelength0.12399Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.03 – 4.818Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:164 field values
Field pathRaw value
codeSASDUZ5
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/nm
project.titleSugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
project.publicationnull
project.statusreleased
project.submitted_date2024-03-27
project.released_date2026-03-27
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experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameGlucokinase-1
experiment.sample.molecule[0].short_nameGLK1
experiment.sample.molecule[0].sequenceMSDPKLTKAVDSICDQFIVTKSKISQLTEYFIDCMEKGLEPCESDISQNKGLPMIPTFVTDKPSGQEHGVTMLAADLGGTNFRVCSVELLGNHEFKIEQEKSKIPTFFFQDDHHVTSKDLFQHMALITHQFLTKHHKDVIQDYKWKMGFTFSYPVDQTSLSSGKLIRWTKGFKIGDTVGQDVVQLFQQELNDIGLSNVHVVALTNDTTGTLLARCYASSDAARAINEPVIGCIFGTGTNGCYMEKLENIHKLDPASREELLSQGKTHMCINTEWGSFDNELNHLPTTSYDIKIDQQFSTNPGFHLFEKRVSGLYLGEILRNILLDLEKQELFDLKESVLKNNPFILTTETLSHIEIDTVENDLQDTRDALLKAADLETTFEERVLIQKLVRAISRRAAFLAAVPIAAILIKTNALNQSYHCQVEVGCDGSVVEHYPGFRSMMRHALALSPIGPEGERDVHLRISKDGSGVGAALCALHANY
experiment.sample.molecule[0].organismKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
experiment.sample.molecule[0].uniprot_codeQ6CUZ3
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last481
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSDPKLTKAVDSICDQFIVTKSKISQLTEYFIDCMEKGLEPCESDISQNKGLPMIPTFVT DKPSGQEHGVTMLAADLGGTNFRVCSVELLGNHEFKIEQEKSKIPTFFFQDDHHVTSKDL FQHMALITHQFLTKHHKDVIQDYKWKMGFTFSYPVDQTSLSSGKLIRWTKGFKIGDTVGQ DVVQLFQQELNDIGLSNVHVVALTNDTTGTLLARCYASSDAARAINEPVIGCIFGTGTNG CYMEKLENIHKLDPASREELLSQGKTHMCINTEWGSFDNELNHLPTTSYDIKIDQQFSTN PGFHLFEKRVSGLYLGEILRNILLDLEKQELFDLKESVLKNNPFILTTETLSHIEIDTVE NDLQDTRDALLKAADLETTFEERVLIQKLVRAISRRAAFLAAVPIAAILIKTNALNQSYH CQVEVGCDGSVVEHYPGFRSMMRHALALSPIGPEGERDVHLRISKDGSGVGAALCALHAN Y
experiment.sample.molecule[0].mw53.757
experiment.sample.molecule[0].total_mw107.514
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name10 mM Tris-HCl, 1 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
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experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
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experiment.sample.purity_methodnull
experiment.sample.nameExtrapolated scattering curve of dimeric Kluyveromyces lactis glucokinase 1 (KlGlk1)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
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experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameLeipzig University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameRenato H.
experiment.contributor[0].contributor_surnameWeiße
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-1736-8085
experiment.concentration_methodnull
experiment.concentration_unitnull
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experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time0.045
experiment.number_of_frames30
experiment.wavelength0.12399
experiment.sample_detector_distance3.0
experiment.concentration_min14.2
experiment.concentration_max1.9
experiment.sample_volumenull
experiment.flow_ratenull
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experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].description
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pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionExtropolation to infinite dilution using ALMERGE 1.1 (r11734) with scattering data of KlGlk1 with the following concentrations: 14.2 mg/mL, 7.3 mg/mL, 3.7 mg/mL, 1.9 mg/mL
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw94.225
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw96.0
porod_mw_errornull
pddf_i00.06502
pddf_i0_errornull
guinier_i00.0660649
guinier_i0_errornull
pddf_rg3.289
pddf_rg_errornull
guinier_rg3.364
guinier_rg_error0.003
pddf_dmax9.94
pddf_dmax_errornull
porod_volume154.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first10
guinier_point_last135
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDUZ5_datloglog_img.png
symmetrynull
last_modified2026-03-27T11:00:12.571151+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDUZ5
statussuccess
started_at2026-08-11T14:59:54.510194+00:00
finished_at2026-08-11T15:00:02.568465+00:00
source_last_modified2026-03-27T11:00:12.571151+01:00
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View the complete summary.json source
{
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  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/nm",
  "project": {
    "title": "Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)",
    "publication": null,
    "status": "released",
    "submitted_date": "2024-03-27",
    "released_date": "2026-03-27"
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    "sample": {
      "molecule": [
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          "long_name": "Glucokinase-1",
          "short_name": "GLK1",
          "sequence": "MSDPKLTKAVDSICDQFIVTKSKISQLTEYFIDCMEKGLEPCESDISQNKGLPMIPTFVTDKPSGQEHGVTMLAADLGGTNFRVCSVELLGNHEFKIEQEKSKIPTFFFQDDHHVTSKDLFQHMALITHQFLTKHHKDVIQDYKWKMGFTFSYPVDQTSLSSGKLIRWTKGFKIGDTVGQDVVQLFQQELNDIGLSNVHVVALTNDTTGTLLARCYASSDAARAINEPVIGCIFGTGTNGCYMEKLENIHKLDPASREELLSQGKTHMCINTEWGSFDNELNHLPTTSYDIKIDQQFSTNPGFHLFEKRVSGLYLGEILRNILLDLEKQELFDLKESVLKNNPFILTTETLSHIEIDTVENDLQDTRDALLKAADLETTFEERVLIQKLVRAISRRAAFLAAVPIAAILIKTNALNQSYHCQVEVGCDGSVVEHYPGFRSMMRHALALSPIGPEGERDVHLRISKDGSGVGAALCALHANY",
          "organism": "Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)",
          "uniprot_code": "Q6CUZ3",
          "uniprot_range_first": 1,
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View the complete manifest.json source
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