SASDV53

Fc-fused PTPRA ECD in PBS buffer

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2024-06-24T14:12:15.468392+02:00
▼

1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Fc-fused PTPRA ECD in PBS buffer

Concentration— – 10.0 Buffer / pH10 mM phosphate, 137 mM NaCl, 2.7 mM KCl / 7.4
Experimental temperature15.0 Facility / beamlineTaiwan Photon Source, NSRRC / 13A
Wavelength0.08265 nmExposure2.0 s × 3

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Receptor-type tyrosine-protein phosphatase alpha
View sequence
NNATTVAPSVGITRLINSSTAEPVKEEAKTSNPTSSLTSLSVAPTFSPNITLGPTYLTTVNSSDSDNGTTRTASTNSIGITISPNGTWLPDNQFTDARTEPWEGNSSTAATTPETFPPSDETDIGGLNDIFEAQKIQWHEILNVKSIDYKDDDDKASITLAAARLADPEEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
proteinHomo sapiens——–—dimerMolecule count 275.0 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11209[3]0.0042–1.253325 1/AHas uncertainty columnMissing 03sasbdb/entries/53/sasdv53/source/SASDV53.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)35.32—Å
i0Guinier0.547737——
i0P(r)0.5656——
mwExperimental270.5—kDa
rgGuinier8.3390.361Å
rgP(r)9.15—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

▼

3. Source Fits and Models Source Fits & Models

Fit 1 · DAMMIF

χ²: 1.738CorMap p: 0.0003Unit: 1/A
ModelName / TypeSoftwareSymmetryModel MWPDB DeclarationSource file
1—dummyDAMMIF dammif (r9988)—212.0—Model Image
▶

4. Source File Index Source Files

▶

5. Experiment Description and Publication Experiment & Publication

▼

6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name10 mM phosphate, 137 mM NaCl, 2.7 mM KClBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2021-08-19Storage / measurement temperature15.0 / 15.0
Exposure time2.0Frames3
Wavelength0.08265Sample-to-detector distance9.0
SourceX-ray synchrotronDetectorEiger X 9M
Facility / beamlineTaiwan Photon Source, NSRRC / 13A · Hsinchu, Taiwan
q range0.042 – 12.533Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
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full_entry_zip:source record and ZIP contents (3 items)
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pddf:source record
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sascif:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDV53
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleHigh Density of N- and O-Glycosylation Shields and Defines the Structural Dynamics of the Intrinsically Disordered Ectodomain of Receptor-type Protein Tyrosine Phosphatase Alpha
project.publication.titleHigh Density of N- and O-Glycosylation Shields and Defines the Structural Dynamics of the Intrinsically Disordered Ectodomain of Receptor-type Protein Tyrosine Phosphatase Alpha
project.publication.author_listChien Y, Wang Y, Sridharan D, Kuo C, Chien C, Uchihashi T, Kato K, Angata T, Meng T, Hsu S, Khoo K
project.publication.journalJACS Au
project.publication.doi10.1021/jacsau.3c00124
project.publication.pmidnull
project.publication.published_date2023 Jun 13
project.statusreleased
project.submitted_date2024-06-24
project.released_date2024-06-24
pddf_datanull
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pddf_plotnull
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experiment.instrument.detector.typenull
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experiment.instrument.detector.resolution75.0
experiment.instrument.nameTaiwan Photon Source, NSRRC
experiment.instrument.cityHsinchu
experiment.instrument.countryTaiwan
experiment.instrument.beamline_name13A
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
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experiment.instrument.xray_energynull
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experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameReceptor-type tyrosine-protein phosphatase alpha
experiment.sample.molecule[0].short_namePTPRA
experiment.sample.molecule[0].sequenceNNATTVAPSVGITRLINSSTAEPVKEEAKTSNPTSSLTSLSVAPTFSPNITLGPTYLTTVNSSDSDNGTTRTASTNSIGITISPNGTWLPDNQFTDARTEPWEGNSSTAATTPETFPPSDETDIGGLNDIFEAQKIQWHEILNVKSIDYKDDDDKASITLAAARLADPEEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw75.0
experiment.sample.molecule[0].total_mw150.0
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name10 mM phosphate, 137 mM NaCl, 2.7 mM KCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameFc-fused PTPRA ECD in PBS buffer
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressTaipei, Taiwan
experiment.contributor[0].affiliation[0].full_nameAcademia Sinica
experiment.contributor[0].affiliation[0].webpagehttps://www.sinica.edu.tw
experiment.contributor[0].contributor_nameYong-Sheng
experiment.contributor[0].contributor_surnameWang
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-4868-4507
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2021-08-19
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time2.0
experiment.number_of_frames3
experiment.wavelength0.08265
experiment.sample_detector_distance9.0
experiment.concentration_minnull
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experiment.flow_ratenull
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experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].bead_radius8.5
fits[0].models[0].lognull
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fits[0].chi_square_value1.738
fits[0].p_value0.0003
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pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
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guinier_point_last41
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDV53_datloglog_img.png
symmetrynull
last_modified2024-06-24T14:12:15.468392+02:00
bragg_peak[]
manifest.json:31 field values
Field pathRaw value
codeSASDV53
statussuccess
started_at2026-08-11T13:53:10.581656+00:00
finished_at2026-08-11T13:53:16.337954+00:00
source_last_modified2024-06-24T14:12:15.468392+02:00
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View the complete summary.json source
{
  "code": "SASDV53",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "High Density of N- and O-Glycosylation Shields and Defines the Structural Dynamics of the Intrinsically Disordered Ectodomain of Receptor-type Protein Tyrosine Phosphatase Alpha",
    "publication": {
      "title": "High Density of N- and O-Glycosylation Shields and Defines the Structural Dynamics of the Intrinsically Disordered Ectodomain of Receptor-type Protein Tyrosine Phosphatase Alpha",
      "author_list": "Chien Y, Wang Y, Sridharan D, Kuo C, Chien C, Uchihashi T, Kato K, Angata T, Meng T, Hsu S, Khoo K",
      "journal": "JACS Au",
      "doi": "10.1021/jacsau.3c00124",
      "pmid": null,
      "published_date": "2023 Jun 13"
    },
    "status": "released",
    "submitted_date": "2024-06-24",
    "released_date": "2024-06-24"
  },
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}
View the complete manifest.json source
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