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SASDVN2

S.aureus Protein Map Eap3 and Eap4 domains (Δ266-476)

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2024-10-07T14:58:23.584073+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · S.aureus Protein Map Eap3 and Eap4 domains (Δ266-476)

Concentration— – 5.0 Buffer / pH20 mM HEPES, 140 mM NaCl / 7.4
Experimental temperature20.0 Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS)
Wavelength0.1127 nmExposure2.0 s × 660

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Protein map
View sequence
NYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK
proteinStaphylococcus aureus (strain Mu50 / ATCC 700699)Q99QS1266–476monomerMolecule count 124.124 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1912[3]0.010389–0.471889 1/AHas uncertainty columnMissing 048sasbdb/entries/n2/sasdvn2/source/SASDVN2.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)8.5—Å
i0Guinier18.6471—arbitrary
i0P(r)18.81—arbitrary
mwExperimental19.7—kDa
mwPorod13.0—kDa
porod_volumePorod28.0—ų
rgGuinier2.4730.41Å
rgP(r)2.57—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES, 140 mM NaClBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-11-17Storage / measurement temperature4.0 / 20.0
Exposure time2.0Frames660
Wavelength0.1127Sample-to-detector distance2.1
SourceX-ray synchrotronDetectorPilatus3 X 2M
Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range0.104 – 4.719Sample volume / flow rate60.0 / 0.65

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json：241 field values
Field pathRaw value
codeSASDVN2
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleS. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.
project.publication.titleS. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.
project.publication.author_listMishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
project.publication.journalJ Biol Chem
project.publication.doi10.1016/j.jbc.2024.107627
project.publication.pmid39098536
project.publication.published_date2024 Aug 3
project.statusreleased
project.submitted_date2024-06-17
project.released_date2024-07-22
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameProtein map
experiment.sample.molecule[0].short_nameEap34
experiment.sample.molecule[0].sequenceNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK
experiment.sample.molecule[0].organismStaphylococcus aureus (strain Mu50 / ATCC 700699)
experiment.sample.molecule[0].uniprot_codeQ99QS1
experiment.sample.molecule[0].uniprot_range_first266
experiment.sample.molecule[0].uniprot_range_last476
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHHGHSNIQIPYTITVNGT SQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVI DLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFN KNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANL INSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLT SQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEI TVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISD LDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK
experiment.sample.molecule[0].mw24.124
experiment.sample.molecule[0].total_mw24.124
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionEap34
experiment.sample.buffer.name20 mM HEPES, 140 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameS.aureus Protein Map Eap3 and Eap4 domains (Δ266-476)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameKansas State University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameNitin
experiment.contributor[0].contributor_surnameMishra
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-8931-7351
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-11-17
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time2.0
experiment.number_of_frames660
experiment.wavelength0.1127
experiment.sample_detector_distance2.1
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume60.0
experiment.flow_rate0.65
experiment.s_min0.104
experiment.s_max4.719
experiment.total_exposure_timenull
experiment.seccolumn40
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pddf_software_version5.0
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tags[]
intensity_unitarbitrary
experimental_mw19.7
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw13.0
porod_mw_errornull
pddf_i018.81
pddf_i0_errornull
guinier_i018.6471
guinier_i0_errornull
pddf_rg2.57
pddf_rg_errornull
guinier_rg2.473
guinier_rg_error0.41
pddf_dmax8.5
pddf_dmax_errornull
porod_volume28.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first8
guinier_point_last77
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i0_calibration_standard_datanull
intensities_log_log_plotSASDVN2_datloglog_img.png
symmetrynull
last_modified2024-10-07T14:58:23.584073+02:00
bragg_peak[]
manifest.json：34 field values
Field pathRaw value
codeSASDVN2
statussuccess
started_at2026-08-11T16:46:24.069541+00:00
finished_at2026-08-11T16:46:32.714073+00:00
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View the complete summary.json source
{
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