Endophilin B1 wild type (0.34mg/ml)

{
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  "status": "Published",
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  "angular_unit": "1/A",
  "project": {
    "title": "Peripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers",
    "publication": {
      "title": "Peripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers",
      "author_list": "Thorlacius A, Rulev M, Sundberg O, Sundborger-Lunna A",
      "journal": "Communications Biology",
      "doi": "10.1038/s42003-025-07610-1",
      "pmid": null,
      "published_date": "2025 Feb 05"
    },
    "status": "released",
    "submitted_date": "2024-07-12",
    "released_date": "2025-01-28"
  },
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVS4.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "point",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
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    "sample": {
      "molecule": [
        {
          "long_name": "Endophilin-B1",
          "short_name": null,
          "sequence": "MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIM\r\nKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIK\r\nCGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLK\r\nKAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQ\r\nCYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSD\r\nLKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITY\r\nLELLN",
          "organism": "Homo sapiens",
          "uniprot_code": "Q9Y371",
          "uniprot_range_first": 1,
          "uniprot_range_last": 365,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIM\nKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIK\nCGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLK\nKAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQ\nCYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSD\nLKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITY\nLELLN",
          "mw": 40.796,
          "total_mw": 81.592,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
      ],
      "buffer": {
        "name": "20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.5 mM DTT",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.1,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Endophilin B1 wild type (0.34mg/ml)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "Uppsala University",
            "webpage": null
          }
        ],
        "contributor_name": "Maksim",
        "contributor_surname": "Rulev",
        "orcid": "https://orcid.org/0000-0001-6103-320X"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2024-03-10",
    "storage_temperature": 4.0,
    "cell_temperature": 4.0,
    "exposure_time": 1.0,
    "number_of_frames": 15,
    "wavelength": 0.09918,
    "sample_detector_distance": 2.827,
    "concentration_min": null,
    "concentration_max": 0.69,
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    "flow_rate": null,
    "s_min": 0.085,
    "s_max": 4.954,
    "total_exposure_time": null,
    "seccolumn": null
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      "software": "DENSS",
      "chi_square_value": 0.8116,
      "p_value": 0.9999,
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": "CAUTION: A significant molecular weight discrepancy is apparent on comparing the quoted experimental molecular weight (98 kDa) and that calculated from the amino acid sequence of the protein dimer (68 kDa).",
  "experiment_description": "Synchrotron SAXS data from solutions of endophilin B1 in 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.5 mM DTT, pH 8.1 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.09918 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.69 mg/ml was measured at 4°C. 15 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "1/cm",
  "experimental_mw": 97.7,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 95.0,
  "porod_mw_error": null,
  "pddf_i0": 65.95,
  "pddf_i0_error": null,
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  "guinier_i0_error": null,
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  "pddf_rg_error": null,
  "guinier_rg": 4.967,
  "guinier_rg_error": 0.609,
  "pddf_dmax": 16.0,
  "pddf_dmax_error": null,
  "porod_volume": 152.0,
  "porod_volume_error": null,
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  "estimated_volume_error": null,
  "guinier_point_first": 2,
  "guinier_point_last": 37,
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  "symmetry": null,
  "last_modified": "2025-02-05T17:23:17.881715+01:00",
  "bragg_peak": []
}

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