SASDVS4

Endophilin B1 wild type (0.34mg/ml)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2025-02-05T17:23:17.881715+01:00

CAUTION: A significant molecular weight discrepancy is apparent on comparing the quoted experimental molecular weight (98 kDa) and that calculated from the amino acid sequence of the protein dimer (68 kDa).

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Endophilin B1 wild type (0.34mg/ml)

浓度— – 0.69 缓冲液 / pH20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.5 mM DTT / 8.1
实验温度4.0 设备 / 束线ESRF / BM29
波长0.09918 nm曝光1.0 s × 15

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Endophilin-B1
查看序列
MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIMKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIKCGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLKKAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQCYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSDLKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITYLELLN
proteinHomo sapiensQ9Y3711–365dimer分子数 240.796 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11000[3]0.008509853–0.4953721 1/A含误差列缺失 0236sasbdb/entries/s4/sasdvs4/source/SASDVS4.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)16.0—Å
i0Guinier66.056—1/cm
i0P(r)65.95—1/cm
mwExperimental97.7—kDa
mwPorod95.0—kDa
porod_volumePorod152.0—ų
rgGuinier4.9670.609Å
rgP(r)5.1—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.5 mM DTT缓冲液浓度—
pH8.1添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2024-03-10储存 / 测量温度4.0 / 4.0
曝光时间1.0帧数15
波长0.09918样品-探测器距离2.827
光源X-ray synchrotron探测器Pilatus3 2M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.085 – 4.954样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/s4/sasdvs4/source/SASDVS4.zipdownloaded991874af30091d637567281794107f0bd5d8cce36dbd6ad0ecab37ead86debdd7910cc下载查看原文件源站
pddfsasbdb/entries/s4/sasdvs4/source/SASDVS4.outdownloaded56589bc63dafd4498b57be0983f3ccae141c2eeb8f68025b6fb928445ce6f615a4eff下载查看原文件源站
sascifsasbdb/entries/s4/sasdvs4/source/SASDVS4.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/s4/sasdvs4/source/summary.jsondownloaded7724d0ac53b8670f70dc168fa16d433dfe1c63d3bde00126a2f4b663702a4280b8fd下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDVS4
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titlePeripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers
project.publication.titlePeripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers
project.publication.author_listThorlacius A, Rulev M, Sundberg O, Sundborger-Lunna A
project.publication.journalCommunications Biology
project.publication.doi10.1038/s42003-025-07610-1
project.publication.pmidnull
project.publication.published_date2025 Feb 05
project.statusreleased
project.submitted_date2024-07-12
project.released_date2025-01-28
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 2M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometrypoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameEndophilin-B1
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceMNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIM KQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIK CGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLK KAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQ CYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSD LKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITY LELLN
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ9Y371
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last365
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIM KQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIK CGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLK KAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQ CYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSD LKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITY LELLN
experiment.sample.molecule[0].mw40.796
experiment.sample.molecule[0].total_mw81.592
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.5 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.1
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEndophilin B1 wild type (0.34mg/ml)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameUppsala University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameMaksim
experiment.contributor[0].contributor_surnameRulev
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-6103-320X
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-03-10
experiment.storage_temperature4.0
experiment.cell_temperature4.0
experiment.exposure_time1.0
experiment.number_of_frames15
experiment.wavelength0.09918
experiment.sample_detector_distance2.827
experiment.concentration_minnull
experiment.concentration_max0.69
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.085
experiment.s_max4.954
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plotnull
fits[0].models[0].softwareDENSS
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fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldensity
fits[0].models[0].software_versionnull
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fits[0].models[0].bead_radiusnull
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fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user1101
fits[0].fit_unit1/A
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fits[0].p_value0.9999
fits[0].fit_residual_plotSASDVS4_fit1_fitresiduals_img.png
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fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionCAUTION: A significant molecular weight discrepancy is apparent on comparing the quoted experimental molecular weight (98 kDa) and that calculated from the amino acid sequence of the protein dimer (68 kDa).
experiment_descriptionSynchrotron SAXS data from solutions of endophilin B1 in 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.5 mM DTT, pH 8.1 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.09918 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.69 mg/ml was measured at 4°C. 15 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unit1/cm
experimental_mw97.7
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw95.0
porod_mw_errornull
pddf_i065.95
pddf_i0_errornull
guinier_i066.056
guinier_i0_errornull
pddf_rg5.1
pddf_rg_errornull
guinier_rg4.967
guinier_rg_error0.609
pddf_dmax16.0
pddf_dmax_errornull
porod_volume152.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first2
guinier_point_last37
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVS4_datloglog_img.png
symmetrynull
last_modified2025-02-05T17:23:17.881715+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDVS4
statussuccess
started_at2026-08-11T14:30:30.407810+00:00
finished_at2026-08-11T14:30:39.298166+00:00
source_last_modified2025-02-05T17:23:17.881715+01:00
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查看完整 summary.json 原文
{
  "code": "SASDVS4",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Peripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers",
    "publication": {
      "title": "Peripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers",
      "author_list": "Thorlacius A, Rulev M, Sundberg O, Sundborger-Lunna A",
      "journal": "Communications Biology",
      "doi": "10.1038/s42003-025-07610-1",
      "pmid": null,
      "published_date": "2025 Feb 05"
    },
    "status": "released",
    "submitted_date": "2024-07-12",
    "released_date": "2025-01-28"
  },
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          "long_name": "Endophilin-B1",
          "short_name": null,
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          "organism": "Homo sapiens",
          "uniprot_code": "Q9Y371",
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          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIM\nKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIK\nCGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLK\nKAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQ\nCYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSD\nLKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITY\nLELLN",
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          "total_mw": 81.592,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
      ],
      "buffer": {
        "name": "20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.5 mM DTT",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.1,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Endophilin B1 wild type (0.34mg/ml)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "Uppsala University",
            "webpage": null
          }
        ],
        "contributor_name": "Maksim",
        "contributor_surname": "Rulev",
        "orcid": "https://orcid.org/0000-0001-6103-320X"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2024-03-10",
    "storage_temperature": 4.0,
    "cell_temperature": 4.0,
    "exposure_time": 1.0,
    "number_of_frames": 15,
    "wavelength": 0.09918,
    "sample_detector_distance": 2.827,
    "concentration_min": null,
    "concentration_max": 0.69,
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    "s_max": 4.954,
    "total_exposure_time": null,
    "seccolumn": null
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          "software": "DENSS",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "density",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDVS4_fit1_model1.mrc",
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          "log": null,
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          "user": 1101
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      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVS4_fit1_fixed_fit_img.png",
      "software": "DENSS",
      "chi_square_value": 0.8116,
      "p_value": 0.9999,
      "fit_residual_plot": "SASDVS4_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDVS4_fit1.fit",
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  "pddf_software": "ATSAS GNOM",
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  "description": "CAUTION: A significant molecular weight discrepancy is apparent on comparing the quoted experimental molecular weight (98 kDa) and that calculated from the amino acid sequence of the protein dimer (68 kDa).",
  "experiment_description": "Synchrotron SAXS data from solutions of endophilin B1 in 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.5 mM DTT, pH 8.1 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.09918 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.69 mg/ml was measured at 4°C. 15 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
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  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
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  "pddf_i0_error": null,
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  "guinier_i0_error": null,
  "pddf_rg": 5.1,
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  "guinier_rg_error": 0.609,
  "pddf_dmax": 16.0,
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  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 2,
  "guinier_point_last": 37,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDVS4_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-02-05T17:23:17.881715+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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