SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245) L223P, L227P and L230P triple mutant: 1667 µM

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    "title": "Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation.",
    "publication": {
      "title": "Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation.",
      "author_list": "Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN",
      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gkaf502",
      "pmid": "40503686",
      "published_date": "2025 Jun 6"
    },
    "status": "released",
    "submitted_date": "2023-09-21",
    "released_date": "2025-02-10"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDVT6.out",
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  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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    "sample": {
      "molecule": [
        {
          "long_name": "Nucleoprotein (L223P, L227P, L230P)",
          "short_name": "N-protein LP mutant",
          "sequence": "GPSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLPLDRPNQPESKMSGKGQQQQGQTWSHPQFEK",
          "organism": "Severe acute respiratory syndrome coronavirus 2",
          "uniprot_code": "P0DTC9",
          "uniprot_range_first": 176,
          "uniprot_range_last": 245,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHG\nKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAG\nLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGS\nQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQ\nQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH\nWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAY\nKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA",
          "mw": 8.325,
          "total_mw": 8.325,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Strep(II)-tagged N-protein 176-245, L223P, L227P, L230P triple mutant. The protein construct used for SAXS contains an additional two amino acids at the N-terminus (GP) as well as the VWSHPQFEK C-terminal tag."
        }
      ],
      "buffer": {
        "name": "100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245) L223P, L227P and L230P triple mutant: 1667 µM",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
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      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Oeiras, Portugal",
            "full_name": "Instituto de Tecnologia Química e Biológica António Xavier, Universidade de Lisboa",
            "webpage": "http://www.itqb.unl.pt"
          }
        ],
        "contributor_name": "Guillem",
        "contributor_surname": "Hernandez",
        "orcid": "https://orcid.org/0000-0003-4438-5611"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-01-29",
    "storage_temperature": 10.0,
    "cell_temperature": 10.0,
    "exposure_time": 2.0,
    "number_of_frames": 10,
    "wavelength": 0.0999,
    "sample_detector_distance": 2.8,
    "concentration_min": null,
    "concentration_max": 14.0,
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    "flow_rate": null,
    "s_min": 0.229,
    "s_max": 5.21,
    "total_exposure_time": null,
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of the SARS-CoV-2 N-protein inter-domain linker triple mutant (IDL, amino acids 176-245; L223P, L227P and L230P) in 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.0999 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 14.00 mg/ml was measured at 10°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
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  "guinier_i0_mw_error": 0.2,
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  "guinier_i0_error": null,
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  "pddf_rg_error": 0.02,
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  "guinier_rg_error": 2.995,
  "pddf_dmax": 12.0,
  "pddf_dmax_error": 0.5,
  "porod_volume": 13.9,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 6,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDVT6_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-06-16T15:30:17.918367+02:00",
  "bragg_peak": []
}

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