查看完整 summary.json 原文
{
"code": "SASDVT6",
"status": "Published",
"type_of_curve": "Single concentration",
"angular_unit": "1/A",
"project": {
"title": "Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation.",
"publication": {
"title": "Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation.",
"author_list": "Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN",
"journal": "Nucleic Acids Res",
"doi": "10.1093/nar/gkaf502",
"pmid": "40503686",
"published_date": "2025 Jun 6"
},
"status": "released",
"submitted_date": "2023-09-21",
"released_date": "2025-02-10"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDVT6.out",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDVT6.dat",
"intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVT6_dat_img.png",
"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVT6_kratky_img.png",
"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVT6_pofr_img.png",
"intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVT6_guinier_img.png",
"sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVT6.sascif",
"experiment": {
"instrument": {
"detector": {
"type": "Dectris",
"name": "Pilatus 1M",
"resolution": 172.0
},
"name": "ESRF",
"city": "Grenoble",
"country": "France",
"beamline_name": "BM29",
"beam_geometry": "",
"type_of_source": "X-ray synchrotron",
"point_source": null,
"line_collimation": null,
"sample_path_length": null,
"line_collimation_slitlength": null,
"line_collimation_integrationwidth": null,
"xray_energy": null,
"beam_profile_ah": null,
"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "Nucleoprotein (L223P, L227P, L230P)",
"short_name": "N-protein LP mutant",
"sequence": "GPSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLPLDRPNQPESKMSGKGQQQQGQTWSHPQFEK",
"organism": "Severe acute respiratory syndrome coronavirus 2",
"uniprot_code": "P0DTC9",
"uniprot_range_first": 176,
"uniprot_range_last": 245,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHG\nKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAG\nLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGS\nQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQ\nQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH\nWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAY\nKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA",
"mw": 8.325,
"total_mw": 8.325,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": "Strep(II)-tagged N-protein 176-245, L223P, L227P, L230P triple mutant. The protein construct used for SAXS contains an additional two amino acids at the N-terminus (GP) as well as the VWSHPQFEK C-terminal tag."
}
],
"buffer": {
"name": "100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 8.0,
"deuteration": null
},
"purity_method": null,
"name": "SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245) L223P, L227P and L230P triple mutant: 1667 µM",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Oeiras, Portugal",
"full_name": "Instituto de Tecnologia Química e Biológica António Xavier, Universidade de Lisboa",
"webpage": "http://www.itqb.unl.pt"
}
],
"contributor_name": "Guillem",
"contributor_surname": "Hernandez",
"orcid": "https://orcid.org/0000-0003-4438-5611"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2023-01-29",
"storage_temperature": 10.0,
"cell_temperature": 10.0,
"exposure_time": 2.0,
"number_of_frames": 10,
"wavelength": 0.0999,
"sample_detector_distance": 2.8,
"concentration_min": null,
"concentration_max": 14.0,
"sample_volume": null,
"flow_rate": null,
"s_min": 0.229,
"s_max": 5.21,
"total_exposure_time": null,
"seccolumn": null
},
"fits": [],
"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
"pddf_software_version": "5.0",
"i0_calibration_standard": null,
"description": null,
"experiment_description": "Synchrotron SAXS data from solutions of the SARS-CoV-2 N-protein inter-domain linker triple mutant (IDL, amino acids 176-245; L223P, L227P and L230P) in 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.0999 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 14.00 mg/ml was measured at 10°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
"intensity_unit": "arbitrary",
"experimental_mw": 8.5,
"experimental_mw_error": null,
"guinier_i0_mw": 12.3,
"guinier_i0_mw_error": 0.2,
"porod_mw": null,
"porod_mw_error": null,
"pddf_i0": 69.41,
"pddf_i0_error": null,
"guinier_i0": 69.5127,
"guinier_i0_error": null,
"pddf_rg": 2.85,
"pddf_rg_error": 0.02,
"guinier_rg": 2.751,
"guinier_rg_error": 2.995,
"pddf_dmax": 12.0,
"pddf_dmax_error": 0.5,
"porod_volume": 13.9,
"porod_volume_error": null,
"estimated_volume": null,
"estimated_volume_error": null,
"guinier_point_first": 1,
"guinier_point_last": 6,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDVT6_datloglog_img.png",
"symmetry": null,
"last_modified": "2025-06-16T15:30:17.918367+02:00",
"bragg_peak": []
}
查看完整 manifest.json 原文
{
"code": "SASDVT6",
"status": "success",
"started_at": "2026-08-11T16:51:56.937572+00:00",
"finished_at": "2026-08-11T16:52:05.335808+00:00",
"source_last_modified": "2025-06-16T15:30:17.918367+02:00",
"files": [
{
"status": "downloaded",
"url": "https://www.sasbdb.org/rest-api/entry/summary/SASDVT6/",
"path": "/data/saxsdb/sasbdb/entries/t6/sasdvt6/source/summary.json",
"size": 6865,
"sha256": "f4d5aa9c1b063ff251dcaab38416c9f81ce3c9447cea64dfb2d5f1f59abc481d"
},
{
"status": "downloaded",
"url": "https://www.sasbdb.org/media/intensities_files/SASDVT6.dat",
"path": "/data/saxsdb/sasbdb/entries/t6/sasdvt6/source/SASDVT6.dat",
"size": 43499,
"sha256": "adf802510413696a15d88a544a235e9a5a7b1577b0c8c8d11eac3a90e28f89ac",
"role": "curve"
},
{
"status": "downloaded",
"url": "https://www.sasbdb.org/media/p_of_R_files/SASDVT6.out",
"path": "/data/saxsdb/sasbdb/entries/t6/sasdvt6/source/SASDVT6.out",
"size": 75393,
"sha256": "660fa30e09aa1142800a82dbf6a30194464df755a0654d6a8341d221d387ce63",
"role": "pddf"
},
{
"status": "not_available",
"url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVT6.sascif",
"path": "/data/saxsdb/sasbdb/entries/t6/sasdvt6/source/SASDVT6.sascif",
"role": "sascif"
},
{
"status": "downloaded",
"url": "https://www.sasbdb.org/media/zip_directories/SASDVT6.zip",
"path": "/data/saxsdb/sasbdb/entries/t6/sasdvt6/source/SASDVT6.zip",
"size": 31348,
"sha256": "3b4f24b3bf2ed0e71c81d04aeaa445ce475ecaab3a74ac89ddf857a9eb6ca3b1",
"role": "full_entry_zip",
"zip_member_count": 2,
"zip_validation": "central_directory_readable"
}
]
}