Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 18 as the linker region

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  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/A",
  "project": {
    "title": "A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation.",
    "publication": {
      "title": "A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation.",
      "author_list": "Nguyen BV, Özden C, Dong K, Koc OC, Torres-Ocampo AP, Dziedzic N, Flaherty D, Huang J, Sankara S, Abromson NL, Tomchick DR, Fissore RA, Chen J, Garman SC, Stratton MM",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-025-63249-w",
      "pmid": "41006217",
      "published_date": "2025 Sep 26"
    },
    "status": "released",
    "submitted_date": "2024-10-05",
    "released_date": "2025-09-03"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDVV7.dat",
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  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVV7_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVV7_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVV7_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVV7.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 X 2M",
        "resolution": 172.0
      },
      "name": "Advanced Light Source (ALS)",
      "city": "Berkeley, CA",
      "country": "USA",
      "beamline_name": "12.3.1 (SIBYLS)",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329)",
          "short_name": "CaMKII Exon 18",
          "sequence": "TRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH",
          "organism": "Homo sapiens",
          "uniprot_code": "Q9UQM7",
          "uniprot_range_first": 7,
          "uniprot_range_last": 478,
          "oligomerization": "dodecamer",
          "molecular_type": "protein",
          "uniprot_sequence": "MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLERE\nARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQIL\nEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYL\nSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTV\nTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLK\nGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEA\nISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIH\nLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH",
          "mw": 52.166,
          "total_mw": 625.992,
          "number_molecules": 12,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "The linker region only contains exon 18."
        }
      ],
      "buffer": {
        "name": "25 mM tris-HCl pH 8.8, 250 mM KCl, 1 mM TCEP, 2% glycerol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": null,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 18 as the linker region",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "university of massachusetts amherst",
            "webpage": null
          }
        ],
        "contributor_name": "Bao",
        "contributor_surname": "Nguyen Viet",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-04-17",
    "storage_temperature": -20.0,
    "cell_temperature": 7.0,
    "exposure_time": 3.0,
    "number_of_frames": 600,
    "wavelength": 0.1127,
    "sample_detector_distance": 2.1,
    "concentration_min": 6.0,
    "concentration_max": 6.0,
    "sample_volume": 55.0,
    "flow_rate": 0.65,
    "s_min": 0.114,
    "s_max": 4.729,
    "total_exposure_time": null,
    "seccolumn": null
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          "pdb_link": [],
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          "type_of_model": "dummy",
          "software_version": "2.3i",
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          "bead_radius": null,
          "log": null,
          "symmetry": "P62",
          "comment": null,
          "user": 1133
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      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVV7_fit1_fixed_fit_img.png",
      "software": "GASBOR",
      "chi_square_value": 1.31,
      "p_value": 0.0,
      "fit_residual_plot": "SASDVV7_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDVV7_fit1.fir",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/Exon18.log",
      "software_version": "2.3i",
      "description": ""
    }
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "6.2.4",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 18 as the linker region in 25 mM tris-HCl pH 8.8, 250 mM KCl, 1 mM TCEP, 2% glycerol were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 6 and 6 mg/ml were measured at 7°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.",
  "tags": [],
  "intensity_unit": "arbitrary",
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  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 15890.0,
  "pddf_i0_error": null,
  "guinier_i0": 15761.0,
  "guinier_i0_error": null,
  "pddf_rg": 6.06,
  "pddf_rg_error": null,
  "guinier_rg": 5.996,
  "guinier_rg_error": 0.634,
  "pddf_dmax": 19.0,
  "pddf_dmax_error": null,
  "porod_volume": 1080.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 20,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDVV7_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-10-07T11:35:26.599738+02:00",
  "bragg_peak": []
}

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