查看完整 summary.json 原文
{
"code": "SASDVV7",
"status": "Published",
"type_of_curve": "Merged",
"angular_unit": "1/A",
"project": {
"title": "A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation.",
"publication": {
"title": "A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation.",
"author_list": "Nguyen BV, Özden C, Dong K, Koc OC, Torres-Ocampo AP, Dziedzic N, Flaherty D, Huang J, Sankara S, Abromson NL, Tomchick DR, Fissore RA, Chen J, Garman SC, Stratton MM",
"journal": "Nat Commun",
"doi": "10.1038/s41467-025-63249-w",
"pmid": "41006217",
"published_date": "2025 Sep 26"
},
"status": "released",
"submitted_date": "2024-10-05",
"released_date": "2025-09-03"
},
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},
"name": "Advanced Light Source (ALS)",
"city": "Berkeley, CA",
"country": "USA",
"beamline_name": "12.3.1 (SIBYLS)",
"beam_geometry": "",
"type_of_source": "X-ray synchrotron",
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},
"sample": {
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{
"long_name": "Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329)",
"short_name": "CaMKII Exon 18",
"sequence": "TRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH",
"organism": "Homo sapiens",
"uniprot_code": "Q9UQM7",
"uniprot_range_first": 7,
"uniprot_range_last": 478,
"oligomerization": "dodecamer",
"molecular_type": "protein",
"uniprot_sequence": "MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLERE\nARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQIL\nEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYL\nSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTV\nTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLK\nGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEA\nISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIH\nLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH",
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"number_molecules": 12,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": "The linker region only contains exon 18."
}
],
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"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": null,
"deuteration": null
},
"purity_method": null,
"name": "Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 18 as the linker region",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": null,
"full_name": "university of massachusetts amherst",
"webpage": null
}
],
"contributor_name": "Bao",
"contributor_surname": "Nguyen Viet",
"orcid": ""
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2023-04-17",
"storage_temperature": -20.0,
"cell_temperature": 7.0,
"exposure_time": 3.0,
"number_of_frames": 600,
"wavelength": 0.1127,
"sample_detector_distance": 2.1,
"concentration_min": 6.0,
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"total_exposure_time": null,
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},
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"software": "GASBOR",
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"model_title": null,
"type_of_model": "dummy",
"software_version": "2.3i",
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"comment": null,
"user": 1133
}
],
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"software": "GASBOR",
"chi_square_value": 1.31,
"p_value": 0.0,
"fit_residual_plot": "SASDVV7_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDVV7_fit1.fir",
"fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/Exon18.log",
"software_version": "2.3i",
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}
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"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
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"i0_calibration_standard": null,
"description": null,
"experiment_description": "Synchrotron SAXS data from solutions of Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 18 as the linker region in 25 mM tris-HCl pH 8.8, 250 mM KCl, 1 mM TCEP, 2% glycerol were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 6 and 6 mg/ml were measured at 7°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.",
"tags": [],
"intensity_unit": "arbitrary",
"experimental_mw": 542.3,
"experimental_mw_error": null,
"guinier_i0_mw": null,
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"porod_mw": null,
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"pddf_i0": 15890.0,
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"guinier_i0": 15761.0,
"guinier_i0_error": null,
"pddf_rg": 6.06,
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"pddf_dmax": 19.0,
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"porod_volume": 1080.0,
"porod_volume_error": null,
"estimated_volume": null,
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"guinier_point_first": 1,
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"pddf_point_first": null,
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"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDVV7_datloglog_img.png",
"symmetry": null,
"last_modified": "2025-10-07T11:35:26.599738+02:00",
"bragg_peak": []
}
查看完整 manifest.json 原文
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"finished_at": "2026-08-11T15:41:11.602704+00:00",
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