Fas-activated serine/threonine kinase/phosphoprotein FASTK (169-549)

{
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  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Human FASTK preferentially binds single-stranded and G-rich RNA.",
    "publication": {
      "title": "Human FASTK preferentially binds single-stranded and G-rich RNA.",
      "author_list": "Dawidziak DM, Dzadz DA, Kuska MI, Kanavalli M, Klimecka MM, Merski M, Bandyra KJ, Górna MW",
      "journal": "FEBS J",
      "doi": "10.1111/febs.70155",
      "pmid": "40598781",
      "published_date": "2025 Jul 1"
    },
    "status": "released",
    "submitted_date": "2024-06-20",
    "released_date": "2024-07-21"
  },
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      "detector": {
        "type": null,
        "name": "Eiger 4M",
        "resolution": 75.0
      },
      "name": "Diamond Light Source",
      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
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      "beam_profile_al": null
    },
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      "molecule": [
        {
          "long_name": "Fas-activated serine/threonine kinase (amino acids 169-549)",
          "short_name": "FASTK",
          "sequence": ">Q14296_FASTKΔN1-168_HUMAN\r\nGFPSDGPLVCALEQERRLRLPPKPPPPLQPLLRGGQGLEAALSCPRFLRYPRQHLISSLAEARPEELTPHVMVLLAQHLARHRLREPQLLEAIAHFLVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGTPHALIVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGEEKYRQDLTVPPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASSATTRDPAQRVVLVLRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPLPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG",
          "organism": "Homo sapiens",
          "uniprot_code": "Q14296",
          "uniprot_range_first": 169,
          "uniprot_range_last": 549,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MRRPRGEPGPRAPRPTEGATCAGPGESWSPSPNSMLRVLLSAQTSPARLSGLLLIPPVQP\nCCLGPSKWGDRPVGGGPSAGPVQGLQRLLEQAKSPGELLRWLGQNPSKVRAHHYSVALRR\nLGQLLGSRPRPPPVEQVTLQDLSQLIIRNCPSFDIHTIHVCLHLAVLLGFPSDGPLVCAL\nEQERRLRLPPKPPPPLQPLLRGGQGLEAALSCPRFLRYPRQHLISSLAEARPEELTPHVM\nVLLAQHLARHRLREPQLLEAIAHFLVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFMPCL\nERILAREAGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGTPHALIVRRYLSL\nLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGEEKYRQ\nDLTVPPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASSATTRDPAQRVVLV\nLRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPLPFEELESQRGLPQLKSYLRQKLQAL\nGLRWGPEGG",
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          "total_mw": 43.032,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Recombinant His6-SUMO-FASTK(169-549) after SUMO cleavage and SEC purification. Selected SEC fraction was analysed by SEC-SAXS."
        }
      ],
      "buffer": {
        "name": "50 mM Tris-HCl, 5% (v/v) Glycerol, 200 mM Ammonium Sulphate, 50 mM NaCl, 0.5 mM TCEP, 10 mM Glutamic acid, 10 mM Arginine;",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Fas-activated serine/threonine kinase/phosphoprotein FASTK (169-549)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "University of Warsaw",
            "webpage": null
          }
        ],
        "contributor_name": "Daria",
        "contributor_surname": "Dawidziak",
        "orcid": "https://orcid.org/0000-0001-7468-5346"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2020-01-31",
    "storage_temperature": 4.0,
    "cell_temperature": 15.0,
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    "wavelength": 0.094,
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          "user": 1083
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  "pddf_software_version": "BIFT from BioXTAS RAW 2.2.1",
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  "guinier_i0_mw_error": null,
  "porod_mw": 55.2,
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  "guinier_rg_error": 0.098,
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  "symmetry": null,
  "last_modified": "2025-07-21T18:20:34.936519+02:00",
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