SASDVW2

Fas-activated serine/threonine kinase/phosphoprotein FASTK (169-549)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2025-07-21T18:20:34.936519+02:00

To obtain P(r) data were processed with BIFT based on q range (1/A) 0.0059 to 0.1604

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Fas-activated serine/threonine kinase/phosphoprotein FASTK (169-549)

浓度— – 5.15 缓冲液 / pH50 mM Tris-HCl, 5% (v/v) Glycerol, 200 mM Ammonium Sulphate, 50 mM NaCl, 0.5 mM TCEP, 10 mM Glutamic acid, 10 mM Arginine; / 8.5
实验温度15.0 设备 / 束线Diamond Light Source / B21
波长0.094 nm曝光3.0 s × 620

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Fas-activated serine/threonine kinase (amino acids 169-549)
查看序列
>Q14296_FASTKΔN1-168_HUMANGFPSDGPLVCALEQERRLRLPPKPPPPLQPLLRGGQGLEAALSCPRFLRYPRQHLISSLAEARPEELTPHVMVLLAQHLARHRLREPQLLEAIAHFLVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGTPHALIVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGEEKYRQDLTVPPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASSATTRDPAQRVVLVLRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPLPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG
proteinHomo sapiensQ14296169–549monomer分子数 143.032 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12566[3]0.0026–0.34 1/A含误差列缺失 0317sasbdb/entries/w2/sasdvw2/source/SASDVW2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)12.020.78Å
i0Guinier0.0147202—1/cm
i0P(r)0.01478038—1/cm
mwExperimental47.9—kDa
mwGuinier I(0)47.9—kDa
mwPorod55.2—kDa
porod_volumePorod66.5—ų
rgGuinier2.8350.098Å
rgP(r)2.930.012Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

拟合 1 · DENSS

χ²:0.0034CorMap p:1.0单位:1/A
模型名称 / 类型软件对称性模型 MWPDB 声明来源文件
1—densityDENSS accessed on 2023.09.06———模型
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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM Tris-HCl, 5% (v/v) Glycerol, 200 mM Ammonium Sulphate, 50 mM NaCl, 0.5 mM TCEP, 10 mM Glutamic acid, 10 mM Arginine;缓冲液浓度—
pH8.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2020-01-31储存 / 测量温度4.0 / 15.0
曝光时间3.0帧数620
波长0.094样品-探测器距离3.693
光源X-ray synchrotron探测器Eiger 4M
机构 / 束线Diamond Light Source / B21 · Didcot, UK
q 范围0.026 – 3.4样品体积 / 流速45.0 / 0.08

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/w2/sasdvw2/source/SASDVW2.zipdownloaded1059889f035a72b74c8d7d59625038d58e7cc59cd904f5cdac81958266a6b2700eea497下载查看原文件源站
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sascifsasbdb/entries/w2/sasdvw2/source/SASDVW2.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/w2/sasdvw2/source/summary.jsondownloaded75082aeaec3efa611114635c99051152138e5f31521a75083b97f958d18af1e8c4ce下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDVW2
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleHuman FASTK preferentially binds single-stranded and G-rich RNA.
project.publication.titleHuman FASTK preferentially binds single-stranded and G-rich RNA.
project.publication.author_listDawidziak DM, Dzadz DA, Kuska MI, Kanavalli M, Klimecka MM, Merski M, Bandyra KJ, Górna MW
project.publication.journalFEBS J
project.publication.doi10.1111/febs.70155
project.publication.pmid40598781
project.publication.published_date2025 Jul 1
project.statusreleased
project.submitted_date2024-06-20
project.released_date2024-07-21
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experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
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experiment.instrument.xray_energynull
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experiment.sample.molecule[0].long_nameFas-activated serine/threonine kinase (amino acids 169-549)
experiment.sample.molecule[0].short_nameFASTK
experiment.sample.molecule[0].sequence>Q14296_FASTKΔN1-168_HUMAN GFPSDGPLVCALEQERRLRLPPKPPPPLQPLLRGGQGLEAALSCPRFLRYPRQHLISSLAEARPEELTPHVMVLLAQHLARHRLREPQLLEAIAHFLVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGTPHALIVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGEEKYRQDLTVPPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASSATTRDPAQRVVLVLRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPLPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ14296
experiment.sample.molecule[0].uniprot_range_first169
experiment.sample.molecule[0].uniprot_range_last549
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMRRPRGEPGPRAPRPTEGATCAGPGESWSPSPNSMLRVLLSAQTSPARLSGLLLIPPVQP CCLGPSKWGDRPVGGGPSAGPVQGLQRLLEQAKSPGELLRWLGQNPSKVRAHHYSVALRR LGQLLGSRPRPPPVEQVTLQDLSQLIIRNCPSFDIHTIHVCLHLAVLLGFPSDGPLVCAL EQERRLRLPPKPPPPLQPLLRGGQGLEAALSCPRFLRYPRQHLISSLAEARPEELTPHVM VLLAQHLARHRLREPQLLEAIAHFLVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFMPCL ERILAREAGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGTPHALIVRRYLSL LDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGEEKYRQ DLTVPPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASSATTRDPAQRVVLV LRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPLPFEELESQRGLPQLKSYLRQKLQAL GLRWGPEGG
experiment.sample.molecule[0].mw43.032
experiment.sample.molecule[0].total_mw43.032
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionRecombinant His6-SUMO-FASTK(169-549) after SUMO cleavage and SEC purification. Selected SEC fraction was analysed by SEC-SAXS.
experiment.sample.buffer.name50 mM Tris-HCl, 5% (v/v) Glycerol, 200 mM Ammonium Sulphate, 50 mM NaCl, 0.5 mM TCEP, 10 mM Glutamic acid, 10 mM Arginine;
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
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experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameFas-activated serine/threonine kinase/phosphoprotein FASTK (169-549)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameUniversity of Warsaw
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameDaria
experiment.contributor[0].contributor_surnameDawidziak
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-7468-5346
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2020-01-31
experiment.storage_temperature4.0
experiment.cell_temperature15.0
experiment.exposure_time3.0
experiment.number_of_frames620
experiment.wavelength0.094
experiment.sample_detector_distance3.693
experiment.concentration_minnull
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experiment.sample_volume45.0
experiment.flow_rate0.08
experiment.s_min0.026
experiment.s_max3.4
experiment.total_exposure_timenull
experiment.seccolumn14
fits[0].models[0].model_plotnull
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fits[0].models[0].comment20 models were generated and 19 were chosen for refinement with DENSS
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fits[0].p_value1.0
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fits[0].description
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pddf_softwareBioXTAS RAW
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i0_calibration_standardnull
descriptionTo obtain P(r) data were processed with BIFT based on q range (1/A) 0.0059 to 0.1604
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw47.9
experimental_mw_errornull
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guinier_point_last322
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVW2_datloglog_img.png
symmetrynull
last_modified2025-07-21T18:20:34.936519+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDVW2
statussuccess
started_at2026-08-11T13:48:10.603379+00:00
finished_at2026-08-11T13:48:21.759235+00:00
source_last_modified2025-07-21T18:20:34.936519+02:00
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查看完整 summary.json 原文
{
  "code": "SASDVW2",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Human FASTK preferentially binds single-stranded and G-rich RNA.",
    "publication": {
      "title": "Human FASTK preferentially binds single-stranded and G-rich RNA.",
      "author_list": "Dawidziak DM, Dzadz DA, Kuska MI, Kanavalli M, Klimecka MM, Merski M, Bandyra KJ, Górna MW",
      "journal": "FEBS J",
      "doi": "10.1111/febs.70155",
      "pmid": "40598781",
      "published_date": "2025 Jul 1"
    },
    "status": "released",
    "submitted_date": "2024-06-20",
    "released_date": "2024-07-21"
  },
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    "sample": {
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        {
          "long_name": "Fas-activated serine/threonine kinase (amino acids 169-549)",
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          "molecular_type": "protein",
          "uniprot_sequence": "MRRPRGEPGPRAPRPTEGATCAGPGESWSPSPNSMLRVLLSAQTSPARLSGLLLIPPVQP\nCCLGPSKWGDRPVGGGPSAGPVQGLQRLLEQAKSPGELLRWLGQNPSKVRAHHYSVALRR\nLGQLLGSRPRPPPVEQVTLQDLSQLIIRNCPSFDIHTIHVCLHLAVLLGFPSDGPLVCAL\nEQERRLRLPPKPPPPLQPLLRGGQGLEAALSCPRFLRYPRQHLISSLAEARPEELTPHVM\nVLLAQHLARHRLREPQLLEAIAHFLVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFMPCL\nERILAREAGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGTPHALIVRRYLSL\nLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGEEKYRQ\nDLTVPPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASSATTRDPAQRVVLV\nLRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPLPFEELESQRGLPQLKSYLRQKLQAL\nGLRWGPEGG",
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        "deuteration": null
      },
      "purity_method": null,
      "name": "Fas-activated serine/threonine kinase/phosphoprotein FASTK (169-549)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
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            "address": null,
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}
查看完整 manifest.json 原文
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