SSRD - Coiled-Coil Tetrahedron with Four Functional Domains (2x SpyCatcher, RFP, DCV)

{
  "code": "SASDW37",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization.",
    "publication": {
      "title": "Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization.",
      "author_list": "Zhou W, Strmšek Ž, Snoj J, Škarabot M, Jerala R",
      "journal": "Small",
      "doi": "10.1002/smll.202502060",
      "pmid": "40442952",
      "published_date": "2025 Jul"
    },
    "status": "released",
    "submitted_date": "2025-02-06",
    "released_date": "2025-08-01"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDW37.dat",
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDW37_pofr_img.png",
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  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 6M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "TET12-2Sc-RFP-DCV",
          "short_name": "SSRD",
          "sequence": ">SSRD\r\nMLEEELKQLEEELQAIEEQLAQLQWKAQARKEKLAQLKEKLSGPGSPEDEIQQLEEEISQLEQKNSELKEKNQELKYGSGPGDIEQELERAKESIRRLEQEVNQERSRMQYLQTLLEKSGPGQLEDKVEELLSKNYHLENEVERLKKLVGSGDSATHIKFSKRDEDGKELAGATMELRDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNGSGLEEELKQLEEELQAIEEQLAQLQWKAQARKEKLAQLKEKLSGPGSPEDEIQQLEEKNSQLKQEISQLEEKNQELKYGSGPGQLEDKVEELLSKNYHLENEVERLKKLVGSGPGSPEDKISQLKEKIQQLKQENQQLEEENSQLEYGSGDSATHIKFSKRDEDGKELAGATMELRDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNGSGSPEDENSQLEEKISQLKQKNSELKEEIQQLEYGSGPGSPEDKISELKEENQQLEQKIQQLKEENSQLEYGSGMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERRHSTGAGSGDIEQELERAKESIRRLEQEVNQERSRMQYLQTLLEKSGPGSPEDKNSELKEEIQQLEEENQQLEEKISELKYGGSGKSGNYSYKRWVFTINNPTFEDYVHVLEFCTLDNCKFAIVGEEKGANGTPHLQGFLNLRSNARAAALEESLGGRAWLSRARGSDEDNEEYCAKESTYLRVGEPVSKGRSSLEHHHHHHHH",
          "organism": "synthetic construct",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "",
          "mw": 109.029,
          "total_mw": 109.029,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "CCPO is short for coiled coil protein origami (CCPO) tetrahedron with 2 SpyCatcher domains, 1 RFP and 1 DCV domain inserted at each of 4 vertices. DCV is an endonuclease ssDNA binding domain originated from Muscovy duck circovirus and  RFP is a red fluorescent protein."
        }
      ],
      "buffer": {
        "name": "20 mM Tris, 150 mM NaCL, 3% glycerol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "SSRD - Coiled-Coil Tetrahedron with Four Functional Domains (2x SpyCatcher, RFP, DCV)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "KI",
            "address": "Ljubljana, Slovenia",
            "full_name": "Kemijski inštitut",
            "webpage": null
          }
        ],
        "contributor_name": "Jaka",
        "contributor_surname": "Snoj",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2024-09-24",
    "storage_temperature": 10.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 3000,
    "wavelength": 0.123985,
    "sample_detector_distance": 3.0,
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    "concentration_max": 10.0,
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    "flow_rate": 0.5,
    "s_min": 0.09,
    "s_max": 2.541,
    "total_exposure_time": null,
    "seccolumn": 47
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          "model_title": null,
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          "user": 1027
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      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDW37_fit1_fixed_fit_img.png",
      "software": "Pepsi-SAXS",
      "chi_square_value": 2.5708,
      "p_value": 0.0,
      "fit_residual_plot": "SASDW37_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDW37_fit1.fit",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/Tet12SN_SSRD_random_very_slow-175-1.log",
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      "description": ""
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": "The experimentally determined protein mass (SEC-MALS) is overestimated due to the presence of a fluorescent protein in the sample. The light scattering laser operates at a wavelength that excites the fluorescent protein, leading to inaccurate signal measurements.",
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
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  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 171.0,
  "porod_mw_error": null,
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  "pddf_i0_error": null,
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  "guinier_i0_error": null,
  "pddf_rg": 4.451,
  "pddf_rg_error": null,
  "guinier_rg": 4.43,
  "guinier_rg_error": 0.011,
  "pddf_dmax": 13.6,
  "pddf_dmax_error": 0.2,
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  "estimated_volume_error": null,
  "guinier_point_first": 22,
  "guinier_point_last": 71,
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  "symmetry": null,
  "last_modified": "2025-08-01T07:48:35.765614+02:00",
  "bragg_peak": []
}

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  "started_at": "2026-08-11T15:21:21.318535+00:00",
  "finished_at": "2026-08-11T15:21:29.384174+00:00",
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