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SASDWG8

NTF2L domain of G3BP1 (residues 1-140) at pH 7.5, 150 mM NaCl

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-07-15T11:21:05.911330+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · NTF2L domain of G3BP1 (residues 1-140) at pH 7.5, 150 mM NaCl

Concentration— – 8.0 Buffer / pH20 mM Tris, 150 mM NaCl, / 7.5
Experimental temperature15.0 Facility / beamlineDiamond Light Source / B21
Wavelength0.09464 nmExposure3.0 s × 600

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
NTF2L domain of Ras GTPase-activating protein-binding protein 1
View sequence
SMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFG
protein—Q132831–141dimerMolecule count 215.964 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
12573[3]0.0045–0.34 1/AHas uncertainty columnMissing 054sasbdb/entries/g8/sasdwg8/source/SASDWG8.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)6.5—Å
i0Guinier0.044775—1/cm
i0P(r)0.04472—1/cm
mwExperimental29.0—kDa
mwPorod31.0—kDa
porod_volumePorod40.0—ų
rgGuinier2.0150.017Å
rgP(r)2.01—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris, 150 mM NaCl,Buffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-05-02Storage / measurement temperature15.0 / 15.0
Exposure time3.0Frames600
Wavelength0.09464Sample-to-detector distance3.7
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineDiamond Light Source / B21 · Didcot, UK
q range0.045 – 3.4Sample volume / flow rate60.0 / 0.08

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
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full_entry_zipsasbdb/entries/g8/sasdwg8/source/SASDWG8.zipdownloaded192648be78c23a14f802e01756d780292becc7a5d915b24a8d7da6c32c5f6b92fdd5e6DownloadView source fileSource site
pddfsasbdb/entries/g8/sasdwg8/source/SASDWG8.outdownloaded1203552f5b041fa3c2f261a7fa043620baa29fc5b31cc62baf826a70a74c04b45a40efDownloadView source fileSource site
sascifsasbdb/entries/g8/sasdwg8/source/SASDWG8.sascifnot_available——DownloadView source fileSource site
summarysasbdb/entries/g8/sasdwg8/source/summary.jsondownloaded7302d3f34a5582bf9b7a09b95e2fb3fd0af3ba1ff96cd8211a3ca42fc381279766f2DownloadView source fileSource site
curve：source record
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full_entry_zip：source record and ZIP contents (6 items)
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pddf：source record
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sascif：source record
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summary：source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json：168 field values
Field pathRaw value
codeSASDWG8
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleSAXS reveals the molecular basis underlying pH-driven G3BP1 conformational dynamics: implications for stress granule formation
project.publicationnull
project.statusapproved
project.submitted_date2025-02-19
project.released_date2026-02-19
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intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWG8_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWG8_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWG8_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDWG8.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNTF2L domain of Ras GTPase-activating protein-binding protein 1
experiment.sample.molecule[0].short_nameNTF2L
experiment.sample.molecule[0].sequenceSMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFG
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codeQ13283
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last141
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKE IHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSV ANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVS NDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQ TVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRD QRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFF QSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAARE GDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ
experiment.sample.molecule[0].mw15.964
experiment.sample.molecule[0].total_mw31.928
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM Tris, 150 mM NaCl,
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameNTF2L domain of G3BP1 (residues 1-140) at pH 7.5, 150 mM NaCl
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[]
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-05-02
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time3.0
experiment.number_of_frames600
experiment.wavelength0.09464
experiment.sample_detector_distance3.7
experiment.concentration_minnull
experiment.concentration_max8.0
experiment.sample_volume60.0
experiment.flow_rate0.08
experiment.s_min0.045
experiment.s_max3.4
experiment.total_exposure_timenull
experiment.seccolumn48
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDWG8_fit1_model1_img.png
fits[0].models[0].softwareDAMMIF
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
fits[0].models[0].software_versionnull
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fits[0].models[0].model_mw27.576
fits[0].models[0].bead_radius1.75
fits[0].models[0].loghttps://www.sasbdb.org/media/log_files/chromixsNTF2-1.log
fits[0].models[0].symmetryP2
fits[0].models[0].commentnull
fits[0].models[0].user1160
fits[0].fit_unit1/A
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fits[0].softwareDAMMIF
fits[0].chi_square_value1.049
fits[0].p_value0.4082
fits[0].fit_residual_plotSASDWG8_fit1_fitresiduals_img.png
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fits[0].software_versionnull
fits[0].description
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fits[1].models[0].softwareSREFLEX
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fits[1].models[0].software_versionnull
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fits[1].models[0].model_mw30.74
fits[1].models[0].bead_radiusnull
fits[1].models[0].lognull
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fits[1].models[0].commentnull
fits[1].models[0].user1160
fits[1].fit_unit1/A
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fits[1].softwareSREFLEX
fits[1].chi_square_value1.073
fits[1].p_value0.4665
fits[1].fit_residual_plotSASDWG8_fit2_fitresiduals_img.png
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fits[1].fit_lognull
fits[1].software_versionnull
fits[1].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw29.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw31.0
porod_mw_errornull
pddf_i00.04472
pddf_i0_errornull
guinier_i00.044775
guinier_i0_errornull
pddf_rg2.01
pddf_rg_errornull
guinier_rg2.015
guinier_rg_error0.017
pddf_dmax6.5
pddf_dmax_errornull
porod_volume40.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first48
guinier_point_last461
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWG8_datloglog_img.png
symmetrynull
last_modified2026-07-15T11:21:05.911330+02:00
bragg_peak[]
manifest.json：34 field values
Field pathRaw value
codeSASDWG8
statussuccess
started_at2026-08-11T15:53:26.463126+00:00
finished_at2026-08-11T15:53:34.958905+00:00
source_last_modified2026-07-15T11:21:05.911330+02:00
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View the complete summary.json source
{
  "code": "SASDWG8",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "SAXS reveals the molecular basis underlying pH-driven G3BP1 conformational dynamics: implications for stress granule formation",
    "publication": null,
    "status": "approved",
    "submitted_date": "2025-02-19",
    "released_date": "2026-02-19"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDWG8.out",
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWG8_pofr_img.png",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDWG8.sascif",
  "experiment": {
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      },
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      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "NTF2L domain of Ras GTPase-activating protein-binding protein 1",
          "short_name": "NTF2L",
          "sequence": "SMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFG",
          "organism": null,
          "uniprot_code": "Q13283",
          "uniprot_range_first": 1,
          "uniprot_range_last": 141,
          "oligomerization": "dimer",
          "molecular_type": "protein",
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          "mw": 15.964,
          "total_mw": 31.928,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
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        "comment": null,
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        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "NTF2L domain of G3BP1 (residues 1-140) at pH 7.5, 150 mM NaCl",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-05-02",
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    "wavelength": 0.09464,
    "sample_detector_distance": 3.7,
    "concentration_min": null,
    "concentration_max": 8.0,
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    "flow_rate": 0.08,
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    "seccolumn": 48
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View the complete manifest.json source
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