SASDX83

T. brucei Canonical Nucleosome Core Particle

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-01-22T11:46:04.158592+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · T. brucei Canonical Nucleosome Core Particle

Concentration— – 1.1 Buffer / pH20 mM HEPES, 150 mM NaCl, 1 mM DTT / 7.5
Experimental temperature15.0 Facility / beamlineDiamond Light Source / B21
Wavelength0.09464 nmExposure3.0 s × 600

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Histone H2A
View sequence
MATPKQAVKKASKGGSSRSVKAGLIFPVGRVGTLLRRGQYARRIGASGAVYMAAVLEYLTAELLELSVKAAAQQTKKTKRLTPRTVTLAVRHDDDLGALLRNVTMSRGGVMPSLNKALAKKQKSGKHAKATPSV
proteinTrypanosoma brucei brucei (strain 927/4 GUTat10.1)Q57YA32–134otherMolecule count 214.212 kDa
Histone H2B
View sequence
ATPKSTPAKTRKEAKKTRRQRKRTWNVYVSRSLRSINSQMSMTSRTMKIVNSFVNDLFERIAAEAATIVRVNRKRTLGARELQTAVRLVLPADLAKHAMAEGTKAVSHASS
proteinTrypanosoma brucei brucei (strain 927/4 GUTat10.1)Q389T12–112otherMolecule count 212.438 kDa
Histone H3, putative
View sequence
SRTKETARTKKTITSKKSKKASKGSDAASGVKTAQRRWRPGTVALREIRQFQRSTDLLLQKAPFQRLVREVSGAQKEGLRFQSSAILAAQEATESYIVSLLADTNRACIHSGRVTIQPKDIHLALCLRGERA
proteinTrypanosoma brucei brucei (strain 927/4 GUTat10.1)Q4GYX72–133otherMolecule count 214.619 kDa
Histone H4
View sequence
AKGKKSGEAKGSQKRQKKVLRENVRGITRGSIRRLARRGGVKRISGVIYDEVRGVLKSFVEGVVRDATAYTEYSRKKTVTAVDVVNALRKRGKILYGYA
proteinTrypanosoma brucei brucei (strain 927/4 GUTat10.1)Q57Z312–100otherMolecule count 211.011 kDa
Widom 601 145 bp DNA - strand 1
View sequence
ATCGATGTATATATCTGACACGTGCCTGGAGACTAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCGTTTAAGCGGTGCTAGAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGATTCTGAT
DNA———–—otherMolecule count 145.055 kDa
Widom 601 145 bp DNA - strand 2
View sequence
ATCAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACCTGTCAGATATATACATCGAT
DNA———–—otherMolecule count 144.543 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
12574[3]0.0045–0.34 1/AHas uncertainty columnMissing 0289sasbdb/entries/83/sasdx83/source/SASDX83.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)11.75—Å
i0Guinier0.0602744—1/cm
i0P(r)0.0599—1/cm
mwExperimental203.028—kDa
porod_volumePorod435.41—ų
rgGuinier4.2620.066Å
rgP(r)4.17—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

▶

5. Experiment Description and Publication Experiment & Publication

▼

6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES, 150 mM NaCl, 1 mM DTTBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-10-10Storage / measurement temperature4.0 / 15.0
Exposure time3.0Frames600
Wavelength0.09464Sample-to-detector distance3.7212
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineDiamond Light Source / B21 · Didcot, UK
q range0.045 – 3.4Sample volume / flow rate50.0 / 0.08

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
curvesasbdb/entries/83/sasdx83/source/SASDX83.datdownloaded103718bcc21af14970e208232bfc8e12dbc6471e78dee4afb6b4ccb466f6f7021b0d5dDownloadView source fileSource site
full_entry_zipsasbdb/entries/83/sasdx83/source/SASDX83.zipdownloaded369909e08edcd12e960da0b880a62c037acba8bee77b896c495ccc35d5c8c4ccd5f7bDownloadView source fileSource site
pddfsasbdb/entries/83/sasdx83/source/SASDX83.outdownloaded429817abab79b36255659fc82f152704122bedf578067510dfbee937a33adbf90aedDownloadView source fileSource site
sascifsasbdb/entries/83/sasdx83/source/SASDX83.sascifnot_available——DownloadView source fileSource site
summarysasbdb/entries/83/sasdx83/source/summary.jsondownloaded8898d8889aff4ba5091b12cba59611f10776c7a209ddb3552b366798db7ae74867c1DownloadView source fileSource site
curve:source record
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full_entry_zip:source record and ZIP contents (2 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:208 field values
Field pathRaw value
codeSASDX83
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleTrypanosome histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin
project.publicationnull
project.statusreleased
project.submitted_date2025-04-02
project.released_date2026-01-22
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDX83.dat
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDX83.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX83_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX83_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDX83_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX83_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDX83.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHistone H2A
experiment.sample.molecule[0].short_nameH2A
experiment.sample.molecule[0].sequenceMATPKQAVKKASKGGSSRSVKAGLIFPVGRVGTLLRRGQYARRIGASGAVYMAAVLEYLTAELLELSVKAAAQQTKKTKRLTPRTVTLAVRHDDDLGALLRNVTMSRGGVMPSLNKALAKKQKSGKHAKATPSV
experiment.sample.molecule[0].organismTrypanosoma brucei brucei (strain 927/4 GUTat10.1)
experiment.sample.molecule[0].uniprot_codeQ57YA3
experiment.sample.molecule[0].uniprot_range_first2
experiment.sample.molecule[0].uniprot_range_last134
experiment.sample.molecule[0].oligomerizationother
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMATPKQAVKKASKGGSSRSVKAGLIFPVGRVGTLLRRGQYARRIGASGAVYMAAVLEYLT AELLELSVKAAAQQTKKTKRLTPRTVTLAVRHDDDLGALLRNVTMSRGGVMPSLNKALAK KQKSGKHAKATPSV
experiment.sample.molecule[0].mw14.212
experiment.sample.molecule[0].total_mw28.424
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.molecule[1].long_nameHistone H2B
experiment.sample.molecule[1].short_nameH2B
experiment.sample.molecule[1].sequenceATPKSTPAKTRKEAKKTRRQRKRTWNVYVSRSLRSINSQMSMTSRTMKIVNSFVNDLFERIAAEAATIVRVNRKRTLGARELQTAVRLVLPADLAKHAMAEGTKAVSHASS
experiment.sample.molecule[1].organismTrypanosoma brucei brucei (strain 927/4 GUTat10.1)
experiment.sample.molecule[1].uniprot_codeQ389T1
experiment.sample.molecule[1].uniprot_range_first2
experiment.sample.molecule[1].uniprot_range_last112
experiment.sample.molecule[1].oligomerizationother
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMATPKSTPAKTRKEAKKTRRQRKRTWNVYVSRSLRSINSQMSMTSRTMKIVNSFVNDLFE RIAAEAATIVRVNRKRTLGARELQTAVRLVLPADLAKHAMAEGTKAVSHASS
experiment.sample.molecule[1].mw12.438
experiment.sample.molecule[1].total_mw24.876
experiment.sample.molecule[1].number_molecules2
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_description
experiment.sample.molecule[2].long_nameHistone H3, putative
experiment.sample.molecule[2].short_nameH3
experiment.sample.molecule[2].sequenceSRTKETARTKKTITSKKSKKASKGSDAASGVKTAQRRWRPGTVALREIRQFQRSTDLLLQKAPFQRLVREVSGAQKEGLRFQSSAILAAQEATESYIVSLLADTNRACIHSGRVTIQPKDIHLALCLRGERA
experiment.sample.molecule[2].organismTrypanosoma brucei brucei (strain 927/4 GUTat10.1)
experiment.sample.molecule[2].uniprot_codeQ4GYX7
experiment.sample.molecule[2].uniprot_range_first2
experiment.sample.molecule[2].uniprot_range_last133
experiment.sample.molecule[2].oligomerizationother
experiment.sample.molecule[2].molecular_typeprotein
experiment.sample.molecule[2].uniprot_sequenceMSRTKETARTKKTITSKKSKKASKGSDAASGVKTAQRRWRPGTVALREIRQFQRSTDLLL QKAPFQRLVREVSGAQKEGLRFQSSAILAAQEATESYIVSLLADTNRACIHSGRVTIQPK DIHLALCLRGERA
experiment.sample.molecule[2].mw14.619
experiment.sample.molecule[2].total_mw29.238
experiment.sample.molecule[2].number_molecules2
experiment.sample.molecule[2].complex_stateFalse
experiment.sample.molecule[2].deuterationnull
experiment.sample.molecule[2].molecule_sourcebiological
experiment.sample.molecule[2].molecule_description
experiment.sample.molecule[3].long_nameHistone H4
experiment.sample.molecule[3].short_nameH4
experiment.sample.molecule[3].sequenceAKGKKSGEAKGSQKRQKKVLRENVRGITRGSIRRLARRGGVKRISGVIYDEVRGVLKSFVEGVVRDATAYTEYSRKKTVTAVDVVNALRKRGKILYGYA
experiment.sample.molecule[3].organismTrypanosoma brucei brucei (strain 927/4 GUTat10.1)
experiment.sample.molecule[3].uniprot_codeQ57Z31
experiment.sample.molecule[3].uniprot_range_first2
experiment.sample.molecule[3].uniprot_range_last100
experiment.sample.molecule[3].oligomerizationother
experiment.sample.molecule[3].molecular_typeprotein
experiment.sample.molecule[3].uniprot_sequenceMAKGKKSGEAKGSQKRQKKVLRENVRGITRGSIRRLARRGGVKRISGVIYDEVRGVLKSF VEGVVRDATAYTEYSRKKTVTAVDVVNALRKRGKILYGYA
experiment.sample.molecule[3].mw11.011
experiment.sample.molecule[3].total_mw22.022
experiment.sample.molecule[3].number_molecules2
experiment.sample.molecule[3].complex_stateFalse
experiment.sample.molecule[3].deuterationnull
experiment.sample.molecule[3].molecule_sourcebiological
experiment.sample.molecule[3].molecule_description
experiment.sample.molecule[4].long_nameWidom 601 145 bp DNA - strand 1
experiment.sample.molecule[4].short_namenull
experiment.sample.molecule[4].sequenceATCGATGTATATATCTGACACGTGCCTGGAGACTAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCGTTTAAGCGGTGCTAGAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGATTCTGAT
experiment.sample.molecule[4].organismnull
experiment.sample.molecule[4].uniprot_codenull
experiment.sample.molecule[4].uniprot_range_firstnull
experiment.sample.molecule[4].uniprot_range_lastnull
experiment.sample.molecule[4].oligomerizationother
experiment.sample.molecule[4].molecular_typeDNA
experiment.sample.molecule[4].uniprot_sequence
experiment.sample.molecule[4].mw45.055
experiment.sample.molecule[4].total_mw45.055
experiment.sample.molecule[4].number_molecules1
experiment.sample.molecule[4].complex_stateFalse
experiment.sample.molecule[4].deuterationnull
experiment.sample.molecule[4].molecule_sourcebiological
experiment.sample.molecule[4].molecule_description
experiment.sample.molecule[5].long_nameWidom 601 145 bp DNA - strand 2
experiment.sample.molecule[5].short_namenull
experiment.sample.molecule[5].sequenceATCAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACCTGTCAGATATATACATCGAT
experiment.sample.molecule[5].organismnull
experiment.sample.molecule[5].uniprot_codenull
experiment.sample.molecule[5].uniprot_range_firstnull
experiment.sample.molecule[5].uniprot_range_lastnull
experiment.sample.molecule[5].oligomerizationother
experiment.sample.molecule[5].molecular_typeDNA
experiment.sample.molecule[5].uniprot_sequence
experiment.sample.molecule[5].mw44.543
experiment.sample.molecule[5].total_mw44.543
experiment.sample.molecule[5].number_molecules1
experiment.sample.molecule[5].complex_stateFalse
experiment.sample.molecule[5].deuterationnull
experiment.sample.molecule[5].molecule_sourcebiological
experiment.sample.molecule[5].molecule_description
experiment.sample.buffer.name20 mM HEPES, 150 mM NaCl, 1 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameT. brucei Canonical Nucleosome Core Particle
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[]
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-10-10
experiment.storage_temperature4.0
experiment.cell_temperature15.0
experiment.exposure_time3.0
experiment.number_of_frames600
experiment.wavelength0.09464
experiment.sample_detector_distance3.7212
experiment.concentration_minnull
experiment.concentration_max1.1
experiment.sample_volume50.0
experiment.flow_rate0.08
experiment.s_min0.045
experiment.s_max3.4
experiment.total_exposure_timenull
experiment.seccolumn14
fits[]
estimated_volume_methodnull
pddf_softwareScÅtter
pddf_software_versionIV
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw203.028
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.0599
pddf_i0_errornull
guinier_i00.0602744
guinier_i0_errornull
pddf_rg4.17
pddf_rg_errornull
guinier_rg4.262
guinier_rg_error0.066
pddf_dmax11.75
pddf_dmax_errornull
porod_volume435.41
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first65
guinier_point_last200
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDX83_datloglog_img.png
symmetrynull
last_modified2026-01-22T11:46:04.158592+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDX83
statussuccess
started_at2026-08-11T13:55:04.929686+00:00
finished_at2026-08-11T13:55:12.100886+00:00
source_last_modified2026-01-22T11:46:04.158592+01:00
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View the complete summary.json source
{
  "code": "SASDX83",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Trypanosome histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin",
    "publication": null,
    "status": "released",
    "submitted_date": "2025-04-02",
    "released_date": "2026-01-22"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDX83.dat",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDX83.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX83_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX83_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDX83_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX83_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDX83.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Eiger 4M",
        "resolution": 75.0
      },
      "name": "Diamond Light Source",
      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
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}
View the complete manifest.json source
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