SASDXH7

GAGE6 60bp dsDNA oligonucleotide

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2026-02-23T09:45:35.560463+01:00

Data was collected at the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus3 S 2M detector at a sample-detector distance of 2.68 m and at a wavelength of λ = 0.107812 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50 μl sample at 2.587 mg/ml was injected onto a GE Superdex 200 5/150 column at 25°C using 150 mM KCl, 20 mM HEPES (pH 7.4), 5 mM MgCl2, 1 mM DTT, 5% glycerol buffer. 400 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. 2sigma errors from the Australian Synchrotron's SAXS/WAXS beamline have been divided by 2 to be compatible with expectations from the ATSAS package This is the 60 bp GAGE6 oligo from Lee et al (2015); Wang et al (2022) which reportedly binds SFPQ. SAXS analysis indicates some degree of curvature which is likely due to the presence of multiple AT repeat regions in the sequence.

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · GAGE6 60bp dsDNA oligonucleotide

浓度— – — 缓冲液 / pH150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT / 7.5
Experimental temperature设备 / 束线Australian Synchrotron / SAXS/WAXS
波长— nm曝光— s × —

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
GAGE6 60bp dsDNA oligo
查看序列
GCCTTCTGCAAAGAAGTCTTGCGCATCTTTTGTGAAGTTTATTTCTAGCTTTTTGATGCT
DNAHomo sapiens—–—monomer分子数 137.101 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
1702[3]0.004532581–0.4921416 1/A含误差列缺失 0154sasbdb/entries/h7/sasdxh7/source/SASDXH7.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)19.5Å
i0Guinier0.00486542arbitrary
i0P(r)0.005087arbitrary
mwExperimental33.1kDa
mwPorod63.0kDa
porod_volumePorod101.0ų
rgGuinier4.7852.34Å
rgP(r)5.52Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

拟合 1 · DAMMIN ATSAS online

χ²:0.357CorMap p:0.6005单位:1/A

DAMMAVER search envelope which had some curvature used as a search model in DAMMIN

模型名称 / 类型软件Symmetry模型 MWPDB 声明来源文件
1dummyDAMMIN ATSAS onlineP1模型 图片 日志

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT缓冲液浓度
pH7.5添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-03-14储存 / 测量温度— / —
曝光时间帧数
波长样品-探测器距离
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线Australian Synchrotron / SAXS/WAXS · Melbourne, Australia
q range0.045 – 4.921样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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full_entry_zipsasbdb/entries/h7/sasdxh7/source/SASDXH7.zipdownloaded27696ebcb8cac73faa1bc206e53902366fe9c27bb7b2f05204737c7ed19c83b512894Download查看原文件源站
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sascifsasbdb/entries/h7/sasdxh7/source/SASDXH7.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/h7/sasdxh7/source/summary.jsondownloaded7887574776a6c103c288336397a2e1e90a906141fb0467e8a6ad883a66fb11f039c1Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDXH7
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleProbing the statistics of sequence-dependent DNA conformations in solution using SAXS.
project.publication.titleProbing the statistics of sequence-dependent DNA conformations in solution using SAXS.
project.publication.author_listKoning HJ, Pullakhandam A, Whitten AE, Bond CS, Peyrard M
project.publication.journalActa Crystallogr D Struct Biol
project.publication.doi10.1107/S2059798325011556
project.publication.pmid41553140
project.publication.published_date2026 Feb 1
project.statusreleased
project.submitted_date2024-09-18
project.released_date2025-08-12
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDXH7.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDXH7.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXH7_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXH7_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXH7_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXH7_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDXH7.sascif
experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAustralian Synchrotron
experiment.instrument.cityMelbourne
experiment.instrument.countryAustralia
experiment.instrument.beamline_nameSAXS/WAXS
experiment.instrument.beam_geometryPoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameGAGE6 60bp dsDNA oligo
experiment.sample.molecule[0].short_nameGAGE6 oligo
experiment.sample.molecule[0].sequenceGCCTTCTGCAAAGAAGTCTTGCGCATCTTTTGTGAAGTTTATTTCTAGCTTTTTGATGCT
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeDNA
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw37.101
experiment.sample.molecule[0].total_mw37.101
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description60 bp GAGE6 dsDNA oligonucleotide from the study 'insight into the Tumor Suppression Mechanism from the Structure of Human Polypyrimidine Splicing Factor (PSF/SFPQ) Complexed with a 30mer RNA from Murine Virus-like 30S Transcript‑1' (Wang et al, 2022).
experiment.sample.buffer.name150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameGAGE6 60bp dsDNA oligonucleotide
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameThe University of Western Australia
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameHeidar
experiment.contributor[0].contributor_surnameKoning
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-03-14
experiment.storage_temperaturenull
experiment.cell_temperaturenull
experiment.exposure_timenull
experiment.number_of_framesnull
experiment.wavelengthnull
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_maxnull
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.045
experiment.s_max4.921
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareDAMMIN
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
fits[0].models[0].software_versionATSAS online
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fits[0].models[0].model_mwnull
fits[0].models[0].bead_radiusnull
fits[0].models[0].loghttps://www.sasbdb.org/media/log_files/GAGE6_2.log
fits[0].models[0].symmetryP1
fits[0].models[0].commentDAMMAVER search area used in DAMMIN
fits[0].models[0].user726
fits[0].fit_unit1/A
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fits[0].softwareDAMMIN
fits[0].chi_square_value0.357
fits[0].p_value0.6005
fits[0].fit_residual_plotSASDXH7_fit1_fitresiduals_img.png
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fits[0].fit_loghttps://www.sasbdb.org/media/fitting_files/log_files/GAGE6_2.log
fits[0].software_versionATSAS online
fits[0].descriptionDAMMAVER search envelope which had some curvature used as a search model in DAMMIN
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionData was collected at the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus3 S 2M detector at a sample-detector distance of 2.68 m and at a wavelength of λ = 0.107812 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50 μl sample at 2.587 mg/ml was injected onto a GE Superdex 200 5/150 column at 25°C using 150 mM KCl, 20 mM HEPES (pH 7.4), 5 mM MgCl2, 1 mM DTT, 5% glycerol buffer. 400 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. 2sigma errors from the Australian Synchrotron's SAXS/WAXS beamline have been divided by 2 to be compatible with expectations from the ATSAS package This is the 60 bp GAGE6 oligo from Lee et al (2015); Wang et al (2022) which reportedly binds SFPQ. SAXS analysis indicates some degree of curvature which is likely due to the presence of multiple AT repeat regions in the sequence.
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw33.1
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw63.0
porod_mw_errornull
pddf_i00.005087
pddf_i0_errornull
guinier_i00.00486542
guinier_i0_errornull
pddf_rg5.52
pddf_rg_errornull
guinier_rg4.785
guinier_rg_error2.34
pddf_dmax19.5
pddf_dmax_errornull
porod_volume101.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first3
guinier_point_last33
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXH7_datloglog_img.png
symmetrynull
last_modified2026-02-23T09:45:35.560463+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDXH7
statussuccess
started_at2026-08-11T16:27:03.644560+00:00
finished_at2026-08-11T16:27:10.642130+00:00
source_last_modified2026-02-23T09:45:35.560463+01:00
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查看完整 summary.json 原文
{
  "code": "SASDXH7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Probing the statistics of sequence-dependent DNA conformations in solution using SAXS.",
    "publication": {
      "title": "Probing the statistics of sequence-dependent DNA conformations in solution using SAXS.",
      "author_list": "Koning HJ, Pullakhandam A, Whitten AE, Bond CS, Peyrard M",
      "journal": "Acta Crystallogr D Struct Biol",
      "doi": "10.1107/S2059798325011556",
      "pmid": "41553140",
      "published_date": "2026 Feb 1"
    },
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    "released_date": "2025-08-12"
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}
查看完整 manifest.json 原文
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