SASDXJ2

Mammalian translation elongation factor eEF1A1

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2025-11-15T14:55:01.726527+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Mammalian translation elongation factor eEF1A1

Concentration— – 1.7 Buffer / pH25 mM Tris HCl, 150 mM NaCl, 6 mM βME, 20% glycerol, 0.01mM GDP, / 7.5
Experimental temperature20.0 Facility / beamlineDepartment of Chemistry, iNANO building, Aarhus Uinversity / Bruker Nanostar w Excillum source
Wavelength0.134 nmExposure1800.0 s × 1

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Mammalian translation elongation factor eEF1A1
View sequence
MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK
protein—P681041–462dimerMolecule count 250.14 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1105[4]0.00981300045–0.415697008 1/AHas uncertainty columnMissing 00sasbdb/entries/j2/sasdxj2/source/SASDXJ2.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)143.1—Å
i0Guinier0.15163—1/cm
i0P(r)0.1440.0011/cm
mwExperimental128.0—kDa
mwGuinier I(0)134.0—kDa
mwPorod123.0—kDa
porod_volumePorod144.24—ų
rgGuinier4.6210.5Å
rgP(r)44.20.1Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25 mM Tris HCl, 150 mM NaCl, 6 mM βME, 20% glycerol, 0.01mM GDP,Buffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-03-20Storage / measurement temperature5.0 / 20.0
Exposure time1800.0Frames1
Wavelength0.134Sample-to-detector distance0.9
SourceX-ray in houseDetectorVÅNTEC-2000
Facility / beamlineDepartment of Chemistry, iNANO building, Aarhus Uinversity / Bruker Nanostar w Excillum source · Aarhus C, Denmark
q range0.098 – 4.157Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (4 items)
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pddf:source record
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sascif:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDXJ2
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleDynamics and structural features of the eEF1A1 and eEF1A2 paralogs
project.publication.titleDynamics and structural features of the eEF1A1 and eEF1A2 paralogs
project.publication.author_listNovosylna O, Shalak V, Dąbrowska K, Patmanidis I, Lozhko D, Bondarchuk T, Schiøtt B, Pedersen J, Knudsen C, Nissen P, Dadlez M, Negrutskii B
project.publication.journalNucleic Acids Research
project.publication.doi10.1093/nar/gkaf1177
project.publication.pmidnull
project.publication.published_date2025 Nov 13
project.statusreleased
project.submitted_date2025-03-19
project.released_date2025-11-15
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experiment.instrument.detector.typeMikroGap gas detector
experiment.instrument.detector.nameVÅNTEC-2000
experiment.instrument.detector.resolution200.0
experiment.instrument.nameDepartment of Chemistry, iNANO building, Aarhus Uinversity
experiment.instrument.cityAarhus C
experiment.instrument.countryDenmark
experiment.instrument.beamline_nameBruker Nanostar w Excillum source
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameMammalian translation elongation factor eEF1A1
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codeP68104
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last462
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVL DKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGV GEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKK IGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTR PTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALS EALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPV LDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPP LGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK
experiment.sample.molecule[0].mw50.14
experiment.sample.molecule[0].total_mw100.28
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name25 mM Tris HCl, 150 mM NaCl, 6 mM βME, 20% glycerol, 0.01mM GDP,
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMammalian translation elongation factor eEF1A1
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameInstitute of Molecular Biology and Genetics National Academy of Sciences of Ukraine
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameDmytro
experiment.contributor[0].contributor_surnameLozhko
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-3251-3164
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-03-20
experiment.storage_temperature5.0
experiment.cell_temperature20.0
experiment.exposure_time1800.0
experiment.number_of_frames1
experiment.wavelength0.134
experiment.sample_detector_distance0.9
experiment.concentration_minnull
experiment.concentration_max1.7
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.098
experiment.s_max4.157
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].softwareCRYSOL
fits[0].chi_square_value2.804
fits[0].p_value0.0911
fits[0].fit_residual_plotSASDXJ2_fit1_fitresiduals_img.png
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fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw128.0
experimental_mw_errornull
guinier_i0_mw134.0
guinier_i0_mw_errornull
porod_mw123.0
porod_mw_errornull
pddf_i00.144
pddf_i0_error0.001
guinier_i00.15163
guinier_i0_errornull
pddf_rg44.2
pddf_rg_error0.1
guinier_rg4.621
guinier_rg_error0.5
pddf_dmax143.1
pddf_dmax_errornull
porod_volume144.24
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last23
pddf_point_first1
pddf_point_last67
i0_calibration_standard_datanull
intensities_log_log_plotSASDXJ2_datloglog_img.png
symmetrynull
last_modified2025-11-15T14:55:01.726527+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDXJ2
statussuccess
started_at2026-08-11T13:39:55.913860+00:00
finished_at2026-08-11T13:40:03.318101+00:00
source_last_modified2025-11-15T14:55:01.726527+01:00
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View the complete summary.json source
{
  "code": "SASDXJ2",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Dynamics and structural features of the eEF1A1 and eEF1A2 paralogs",
    "publication": {
      "title": "Dynamics and structural features of the eEF1A1 and eEF1A2 paralogs",
      "author_list": "Novosylna O, Shalak V, Dąbrowska K, Patmanidis I, Lozhko D, Bondarchuk T, Schiøtt B, Pedersen J, Knudsen C, Nissen P, Dadlez M, Negrutskii B",
      "journal": "Nucleic Acids Research",
      "doi": "10.1093/nar/gkaf1177",
      "pmid": null,
      "published_date": "2025 Nov 13"
    },
    "status": "released",
    "submitted_date": "2025-03-19",
    "released_date": "2025-11-15"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDXJ2.out",
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  "experiment": {
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        "name": "VÅNTEC-2000",
        "resolution": 200.0
      },
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      "city": "Aarhus C",
      "country": "Denmark",
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      "beam_geometry": null,
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    "sample": {
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          "long_name": "Mammalian translation elongation factor eEF1A1",
          "short_name": null,
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          "organism": null,
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          "uniprot_range_first": 1,
          "uniprot_range_last": 462,
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          "molecular_type": "protein",
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          "mw": 50.14,
          "total_mw": 100.28,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
      ],
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