SASDXL6

Histone deacetylase 5 (HDAC5)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2026-02-27T13:41:15.416584+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Histone deacetylase 5 (HDAC5)

浓度— – 2.15 缓冲液 / pH25 mM HEPES, 150 mM KCl, 1 mM DTT, / 7.5
Experimental temperature15.0 设备 / 束线Center for Structural Studies, Heinrich-Heine-University / Xenocs Xeuss 2.0 Q-Xoom
波长0.154 nm曝光600.0 s × 24

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Histone deacetylase 5
查看序列
MLGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPALENLYFQ
proteinHomo sapiensQ9UQL6655–1122monomer分子数 151.059 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
1800[3]0.128498–4.795312 1/nm含误差列缺失 037sasbdb/entries/l6/sasdxl6/source/SASDXL6.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.1nm
i0Guinier0.03616691/cm
i0P(r)0.036151/cm
mwExperimental52.9kDa
mwGuinier I(0)49.85kDa
mwPorod50.0kDa
porod_volumePorod82.47nm³
rgGuinier2.810.029nm
rgP(r)2.891nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM HEPES, 150 mM KCl, 1 mM DTT,缓冲液浓度
pH7.5添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2024-02-29储存 / 测量温度10.0 / 15.0
曝光时间600.0帧数24
波长0.154样品-探测器距离0.55
光源X-ray in house探测器Pilatus3 R 300K
机构 / 束线Center for Structural Studies, Heinrich-Heine-University / Xenocs Xeuss 2.0 Q-Xoom · Düsseldorf, Germany
q range0.128 – 4.795样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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pddfsasbdb/entries/l6/sasdxl6/source/SASDXL6.outdownloaded70304b306ee5d26b8160b6f9679c2b3d0e5e5c9a7e2cb14d823232418eaa98a767fdfDownload查看原文件源站
sascifsasbdb/entries/l6/sasdxl6/source/SASDXL6.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/l6/sasdxl6/source/summary.jsondownloaded69723ad68d1c9917057e2b1f48bce28d62fc5adafea9651761f55bbb2dc24985be21Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDXL6
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleIn vitro characterization of the catalytic domain of human histone deacetylase 5
project.publication.titleIn vitro characterization of the catalytic domain of human histone deacetylase 5
project.publication.author_listMammen C, Hornung F, Anzenhofer C, Schumacher J, Reiners J, Li J, Mazzone F, Bilsing F, Kassack M, Kurz T, Smits S
project.publication.journalScientific Reports
project.publication.doi10.1038/s41598-026-37633-5
project.publication.pmidnull
project.publication.published_date2026 Feb 27
project.statusreleased
project.submitted_date2025-07-21
project.released_date2025-11-05
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDXL6.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDXL6.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXL6_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXL6_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXL6_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXL6_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDXL6.sascif
experiment.instrument.detector.typepixel counting
experiment.instrument.detector.namePilatus3 R 300K
experiment.instrument.detector.resolution172.0
experiment.instrument.nameCenter for Structural Studies, Heinrich-Heine-University
experiment.instrument.cityDüsseldorf
experiment.instrument.countryGermany
experiment.instrument.beamline_nameXenocs Xeuss 2.0 Q-Xoom
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHistone deacetylase 5
experiment.sample.molecule[0].short_nameHDAC5
experiment.sample.molecule[0].sequenceMLGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPALENLYFQ
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ9UQL6
experiment.sample.molecule[0].uniprot_range_first655
experiment.sample.molecule[0].uniprot_range_last1122
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELR GALVGSVDPTLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQ QEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIAS TEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKL PLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEI TGAGPGASSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQ FSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMST SSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATS MRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGE LPRQPTTHPEETEEELTEQQEVLLGEGALTMPREGSTESESTQEDLEEEDEEDDGEEEED CIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQVYQAPLSLATVPHQALGRTQ SSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQE TGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLP CGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAM GFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNF FPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVS AGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVS ALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGET EEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPAL
experiment.sample.molecule[0].mw51.059
experiment.sample.molecule[0].total_mw51.059
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionLast amino acids (ENLYFQ) are the TEV cleavage site.
experiment.sample.buffer.name25 mM HEPES, 150 mM KCl, 1 mM DTT,
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHistone deacetylase 5 (HDAC5)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressDüsseldorf, Germany
experiment.contributor[0].affiliation[0].full_nameHeinrich Heine University Düsseldorf
experiment.contributor[0].affiliation[0].webpagehttps://www.uni-duesseldorf.de
experiment.contributor[0].contributor_nameJens
experiment.contributor[0].contributor_surnameReiners
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-02-29
experiment.storage_temperature10.0
experiment.cell_temperature15.0
experiment.exposure_time600.0
experiment.number_of_frames24
experiment.wavelength0.154
experiment.sample_detector_distance0.55
experiment.concentration_minnull
experiment.concentration_max2.15
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.128
experiment.s_max4.795
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw52.9
experimental_mw_errornull
guinier_i0_mw49.85
guinier_i0_mw_errornull
porod_mw50.0
porod_mw_errornull
pddf_i00.03615
pddf_i0_errornull
guinier_i00.0361669
guinier_i0_errornull
pddf_rg2.891
pddf_rg_errornull
guinier_rg2.81
guinier_rg_error0.029
pddf_dmax10.1
pddf_dmax_errornull
porod_volume82.47
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last57
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXL6_datloglog_img.png
symmetrynull
last_modified2026-02-27T13:41:15.416584+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDXL6
statussuccess
started_at2026-08-11T15:12:18.925620+00:00
finished_at2026-08-11T15:12:26.185033+00:00
source_last_modified2026-02-27T13:41:15.416584+01:00
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查看完整 summary.json 原文
{
  "code": "SASDXL6",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "In vitro characterization of the catalytic domain of human histone deacetylase 5",
    "publication": {
      "title": "In vitro characterization of the catalytic domain of human histone deacetylase 5",
      "author_list": "Mammen C, Hornung F, Anzenhofer C, Schumacher J, Reiners J, Li J, Mazzone F, Bilsing F, Kassack M, Kurz T, Smits S",
      "journal": "Scientific Reports",
      "doi": "10.1038/s41598-026-37633-5",
      "pmid": null,
      "published_date": "2026 Feb 27"
    },
    "status": "released",
    "submitted_date": "2025-07-21",
    "released_date": "2025-11-05"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDXL6.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDXL6.dat",
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  "experiment": {
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        "name": "Pilatus3 R 300K",
        "resolution": 172.0
      },
      "name": "Center for Structural Studies, Heinrich-Heine-University",
      "city": "Düsseldorf",
      "country": "Germany",
      "beamline_name": "Xenocs Xeuss 2.0 Q-Xoom",
      "beam_geometry": null,
      "type_of_source": "X-ray in house",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Histone deacetylase 5",
          "short_name": "HDAC5",
          "sequence": "MLGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPALENLYFQ",
          "organism": "Homo sapiens",
          "uniprot_code": "Q9UQL6",
          "uniprot_range_first": 655,
          "uniprot_range_last": 1122,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELR\nGALVGSVDPTLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQ\nQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIAS\nTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKL\nPLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEI\nTGAGPGASSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQ\nFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMST\nSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATS\nMRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGE\nLPRQPTTHPEETEEELTEQQEVLLGEGALTMPREGSTESESTQEDLEEEDEEDDGEEEED\nCIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQVYQAPLSLATVPHQALGRTQ\nSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQE\nTGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLP\nCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAM\nGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNF\nFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVS\nAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVS\nALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGET\nEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPAL",
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          "molecule_source": "biological",
          "molecule_description": "Last amino acids (ENLYFQ) are the TEV cleavage site."
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        "concentration_unit": null,
        "comment": null,
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        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Histone deacetylase 5 (HDAC5)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Düsseldorf, Germany",
            "full_name": "Heinrich Heine University Düsseldorf",
            "webpage": "https://www.uni-duesseldorf.de"
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        "contributor_name": "Jens",
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        "orcid": ""
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2024-02-29",
    "storage_temperature": 10.0,
    "cell_temperature": 15.0,
    "exposure_time": 600.0,
    "number_of_frames": 24,
    "wavelength": 0.154,
    "sample_detector_distance": 0.55,
    "concentration_min": null,
    "concentration_max": 2.15,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.128,
    "s_max": 4.795,
    "total_exposure_time": null,
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  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "1/cm",
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  "experimental_mw_error": null,
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  "guinier_i0_mw_error": null,
  "porod_mw": 50.0,
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  "pddf_i0": 0.03615,
  "pddf_i0_error": null,
  "guinier_i0": 0.0361669,
  "guinier_i0_error": null,
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  "pddf_rg_error": null,
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  "guinier_rg_error": 0.029,
  "pddf_dmax": 10.1,
  "pddf_dmax_error": null,
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  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 57,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDXL6_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-02-27T13:41:15.416584+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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