SASDYU3

N-terminal truncation mutant, of the conjugative protein of the F plasmid Type IV Secretion System, TraW.

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-03-18T13:59:44.111420+01:00

Synchrotron SAXS data from solutions of a N-terminal truncation mutant of TraW in 20 mM HEPES, 100 mM NaCl, 5% glycerol, pH 7 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS; Argonne National Laboratory USA) using a Eiger2 XE 9M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 350.00 μl sample at 13 mg/ml was injected at a 0.5 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 22°C. 3175 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · N-terminal truncation mutant, of the conjugative protein of the F plasmid Type IV Secretion System, TraW.

浓度— – 13.0 缓冲液 / pH20 mM HEPES, 100 mM NaCl, 5% glycerol / 7.0
Experimental temperature22.0 设备 / 束线Advanced Photon Source (APS), Argonne National Laboratory / BioCAT 18ID
波长0.1033 nm曝光0.7 s × 3175

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
TraW (Δ1-67)
查看序列
LRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEGRVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK
proteinEscherichia coli (strain K12)P1847268–210monomer分子数 119.9 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
11696[3]0.0029028396–0.417082465 1/A含误差列缺失 04sasbdb/entries/u3/sasdyu3/source/SASDYU3.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.0Å
i0Guinier1.6174
i0P(r)1.6130.0017
mwExperimental20.0kDa
mwPorod20.7kDa
rgGuinier2.180.093Å
rgP(r)2.180.074Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 100 mM NaCl, 5% glycerol缓冲液浓度
pH7.0添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2022-11-17储存 / 测量温度4.0 / 22.0
曝光时间0.7帧数3175
波长0.1033样品-探测器距离3.7
光源X-ray synchrotron探测器Eiger2 XE 9M
机构 / 束线Advanced Photon Source (APS), Argonne National Laboratory / BioCAT 18ID · Lemont, IL, USA
q range0.029 – 4.171样品体积 / 流速350.0 / 0.5

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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full_entry_zipsasbdb/entries/u3/sasdyu3/source/SASDYU3.zipdownloaded899897e35dede0269591c3c73543e7854aa2e0dc782ba894706903c18a47859c375c6Download查看原文件源站
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sascifsasbdb/entries/u3/sasdyu3/source/SASDYU3.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/u3/sasdyu3/source/summary.jsondownloaded7200690c378c44329c738c07ab7afa03d84166bd45ef55b339f555dd7bbd0577ef58Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:150 个字段值
字段路径原始值
codeSASDYU3
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleSolution characterization of TraW, a regulatory protein of the F plasmid type 4 secretion system
project.publication.titleSolution characterization of TraW, a regulatory protein of the F plasmid type 4 secretion system
project.publication.author_listRodriguez C, Audette G
project.publication.journalStructural Dynamics
project.publication.doi10.1063/4.0001201
project.publication.pmidnull
project.publication.published_date2026 Mar 17
project.statusreleased
project.submitted_date2025-12-10
project.released_date2026-03-18
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intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDYU3.dat
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intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYU3_kratky_img.png
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger2 XE 9M
experiment.instrument.detector.resolution72.0
experiment.instrument.nameAdvanced Photon Source (APS), Argonne National Laboratory
experiment.instrument.cityLemont, IL
experiment.instrument.countryUSA
experiment.instrument.beamline_nameBioCAT 18ID
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameTraW (Δ1-67)
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEGRVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK
experiment.sample.molecule[0].organismEscherichia coli (strain K12)
experiment.sample.molecule[0].uniprot_codeP18472
experiment.sample.molecule[0].uniprot_range_first68
experiment.sample.molecule[0].uniprot_range_last210
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLTALEQSGEMGRKMDAFK ERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEGRVFARQGEVMNPLQYVPF NQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKSLDSRVYFDQNGVLCQRL GIDQVPARVSAVPGDRFLKVEFIPAEEGRK
experiment.sample.molecule[0].mw19.9
experiment.sample.molecule[0].total_mw19.9
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM HEPES, 100 mM NaCl, 5% glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameN-terminal truncation mutant, of the conjugative protein of the F plasmid Type IV Secretion System, TraW.
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[]
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2022-11-17
experiment.storage_temperature4.0
experiment.cell_temperature22.0
experiment.exposure_time0.7
experiment.number_of_frames3175
experiment.wavelength0.1033
experiment.sample_detector_distance3.7
experiment.concentration_minnull
experiment.concentration_max13.0
experiment.sample_volume350.0
experiment.flow_rate0.5
experiment.s_min0.029
experiment.s_max4.171
experiment.total_exposure_timenull
experiment.seccolumn3
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fits[0].models[0].softwareDAMMIN
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
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fits[0].models[0].bead_radiusnull
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fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user1301
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fits[0].chi_square_value1.271
fits[0].p_value0.0859
fits[0].fit_residual_plotSASDYU3_fit1_fitresiduals_img.png
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fits[0].software_version3.2.1
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version3.2.1
i0_calibration_standardnull
descriptionSynchrotron SAXS data from solutions of a N-terminal truncation mutant of TraW in 20 mM HEPES, 100 mM NaCl, 5% glycerol, pH 7 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS; Argonne National Laboratory USA) using a Eiger2 XE 9M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 350.00 μl sample at 13 mg/ml was injected at a 0.5 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 22°C. 3175 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
experiment_description-
tags[]
intensity_unitnull
experimental_mw20.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw20.7
porod_mw_errornull
pddf_i01.613
pddf_i0_error0.0017
guinier_i01.6174
guinier_i0_errornull
pddf_rg2.18
pddf_rg_error0.074
guinier_rg2.18
guinier_rg_error0.093
pddf_dmax8.0
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first13
guinier_point_last321
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDYU3_datloglog_img.png
symmetrynull
last_modified2026-03-18T13:59:44.111420+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDYU3
statussuccess
started_at2026-08-11T16:30:29.217759+00:00
finished_at2026-08-11T16:30:38.209461+00:00
source_last_modified2026-03-18T13:59:44.111420+01:00
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查看完整 summary.json 原文
{
  "code": "SASDYU3",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Solution characterization of TraW, a regulatory protein of the F plasmid type 4 secretion system",
    "publication": {
      "title": "Solution characterization of TraW, a regulatory protein of the F plasmid type 4 secretion system",
      "author_list": "Rodriguez C, Audette G",
      "journal": "Structural Dynamics",
      "doi": "10.1063/4.0001201",
      "pmid": null,
      "published_date": "2026 Mar 17"
    },
    "status": "released",
    "submitted_date": "2025-12-10",
    "released_date": "2026-03-18"
  },
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查看完整 manifest.json 原文
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