SASDZ98

Engineered fusion of derivative of S.aureus LytN (CHAP domain) and derivative of S.simulans lysostaphin (Fc-L1-CHAP(opt)-LSN(HEK))

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-07-23T17:37:52.565046+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Engineered fusion of derivative of S.aureus LytN (CHAP domain) and derivative of S.simulans lysostaphin (Fc-L1-CHAP(opt)-LSN(HEK))

浓度— – 5.0 缓冲液 / pH20 mM HEPES, 150 mM NaCl, pH 7.0 / 7.0
实验温度25.0 设备 / 束线PETRA III / EMBL P12
波长0.124 nm曝光0.5 s × 1800

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Fc-L1-CHAP(opt)-LSN(HEK)
查看序列
GPEPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEAAAKEAAAKEAAAKEAAAKALEAEAAAKEAAAKEAAAKEAAAKASGSSTLNYLKSLEGKGWDFDGSFGWQCFDLVNVYWNHLYGHGLKGDYAKDIPYANNFNNEAKIYKNTPTFKAEPGDLVVFSGRYGGGYGHTAIVLNGNYDGKLQKFQSLDQNWYGGGERKAEVAHKVVHNYENDMIFIRPFKKAGGAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNPTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWQKSTNTLGVLWGTIK
proteinsynthetic construct——–—dimer分子数 273.856 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11048[3]0.06180079–2.999013 1/nm含误差列缺失 00sasbdb/entries/98/sasdz98/source/SASDZ98.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)40.0—nm
i0Guinier11653.1—arbitrary
i0P(r)12332.32—arbitrary
mwExperimental120.0—kDa
rgGuinier7.30.1nm
rgP(r)8.823—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 150 mM NaCl, pH 7.0缓冲液浓度—
pH7.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2025-04-06储存 / 测量温度25.0 / 25.0
曝光时间0.5帧数1800
波长0.124样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 6M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.062 – 2.999样品体积 / 流速25.0 / 0.35

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/98/sasdz98/source/SASDZ98.zipdownloaded16230b7c31b7179f630017260690976f546d0aa7e79c144e7d431f243dff11c94f8b7下载查看原文件源站
pddfsasbdb/entries/98/sasdz98/source/SASDZ98.outdownloaded74298ee3d8711769562b2318d4f7895e1f7550701e74474edb5c43ad57028622daaa下载查看原文件源站
sascifsasbdb/entries/98/sasdz98/source/SASDZ98.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/98/sasdz98/source/summary.jsondownloaded5348a0e53e306c03160f21d76a30a2fa344f0a60f7a78d3294e5f6355de0cc8ec16e下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:123 个字段值
字段路径原始值
codeSASDZ98
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleSEC-SAXS data for Fc-CHAP-LSN
project.publicationnull
project.statusreleased
project.submitted_date2026-05-13
project.released_date2026-07-23
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pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZ98_pofr_img.png
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 6M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameFc-L1-CHAP(opt)-LSN(HEK)
experiment.sample.molecule[0].short_nameL1829
experiment.sample.molecule[0].sequenceGPEPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEAAAKEAAAKEAAAKEAAAKALEAEAAAKEAAAKEAAAKEAAAKASGSSTLNYLKSLEGKGWDFDGSFGWQCFDLVNVYWNHLYGHGLKGDYAKDIPYANNFNNEAKIYKNTPTFKAEPGDLVVFSGRYGGGYGHTAIVLNGNYDGKLQKFQSLDQNWYGGGERKAEVAHKVVHNYENDMIFIRPFKKAGGAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNPTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWQKSTNTLGVLWGTIK
experiment.sample.molecule[0].organismsynthetic construct
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw73.856
experiment.sample.molecule[0].total_mw147.712
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionhuman IgG1 Fc domain connected via a helical linker to a genetic fusion of the CHAP domain of an engineered derivative of S.aureus LytN and an engineered derivative of S.simulans lysostaphin
experiment.sample.buffer.name20 mM HEPES, 150 mM NaCl, pH 7.0
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEngineered fusion of derivative of S.aureus LytN (CHAP domain) and derivative of S.simulans lysostaphin (Fc-L1-CHAP(opt)-LSN(HEK))
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[]
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-04-06
experiment.storage_temperature25.0
experiment.cell_temperature25.0
experiment.exposure_time0.5
experiment.number_of_frames1800
experiment.wavelength0.124
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume25.0
experiment.flow_rate0.35
experiment.s_min0.062
experiment.s_max2.999
experiment.total_exposure_timenull
experiment.seccolumn49
fits[]
estimated_volume_methodnull
pddf_softwareOther
pddf_software_versionGNOM
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw120.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i012332.32
pddf_i0_errornull
guinier_i011653.1
guinier_i0_errornull
pddf_rg8.823
pddf_rg_errornull
guinier_rg7.3
guinier_rg_error0.1
pddf_dmax40.0
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first12
guinier_point_last32
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDZ98_datloglog_img.png
symmetrynull
last_modified2026-07-23T17:37:52.565046+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDZ98
statussuccess
started_at2026-08-11T15:48:49.272770+00:00
finished_at2026-08-11T15:48:56.774887+00:00
source_last_modified2026-07-23T17:37:52.565046+02:00
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files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDZ98",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "SEC-SAXS data for Fc-CHAP-LSN",
    "publication": null,
    "status": "released",
    "submitted_date": "2026-05-13",
    "released_date": "2026-07-23"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDZ98.dat",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDZ98.dat",
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZ98_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZ98_guinier_img.png",
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      "beam_geometry": null,
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      },
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}
查看完整 manifest.json 原文
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