查看完整 summary.json 原文
{
"code": "SASDZV5",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/A",
"project": {
"title": "The Rho guanine-nucleotide exchange factor P-Rex2 exhibits structural and regulatory features distinct from the related RhoGEF P-Rex1",
"publication": {
"title": "The Rho guanine-nucleotide exchange factor P-Rex2 exhibits structural and regulatory features distinct from the related RhoGEF P-Rex1",
"author_list": "Anderson L, Marde R, Muma G, Nayak V, Phan C, Li S, Cash J",
"journal": "Journal of Biological Chemistry",
"doi": "10.1016/j.jbc.2026.113229",
"pmid": null,
"published_date": "2026 Jul"
},
"status": "released",
"submitted_date": "2026-04-03",
"released_date": "2026-07-23"
},
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"name": "Advanced Light Source (ALS)",
"city": "Berkeley, CA",
"country": "USA",
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{
"long_name": "Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem",
"short_name": "P-Rex2 DH/PH tandem",
"sequence": "GAMDPMSEDSRGDSRAESAKDLEKQLRLRVCVLSELQKTERDYVGTLEFLVSAFLHRMNQCAASKVDKNVTEETVKMLFSNIEDILAVHKEFLKVVEECLHPEPNAQQEVGTCFLHFKDKFRIYDEYCSNHEKAQKLLLELNKIRTIRTFLLNCMLLGGRKNTDVPLEGYLVTPIQRICKYPLILKELLKRTPRKHSDYAAVMEALQAMKAVCSNINEAKRQMEKLEVLEEWQSHIEGWEGSNITDTCTEMLMCGVLLKISSGNIQERVFFLFDNLLVYCKRKHRRLKNSKASTDGHRYLFRGRINTEVMEVENVDDGTADFHSSGHIVVNGWKIHNTAKNKWFVCMAKTPEEKHEWFEAILKERERRKGLKLGMEQDTWVM",
"organism": "Homo sapiens",
"uniprot_code": "Q70Z35",
"uniprot_range_first": 1,
"uniprot_range_last": 377,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MSEDSRGDSRAESAKDLEKQLRLRVCVLSELQKTERDYVGTLEFLVSAFLHRMNQCAASK\nVDKNVTEETVKMLFSNIEDILAVHKEFLKVVEECLHPEPNAQQEVGTCFLHFKDKFRIYD\nEYCSNHEKAQKLLLELNKIRTIRTFLLNCMLLGGRKNTDVPLEGYLVTPIQRICKYPLIL\nKELLKRTPRKHSDYAAVMEALQAMKAVCSNINEAKRQMEKLEVLEEWQSHIEGWEGSNIT\nDTCTEMLMCGVLLKISSGNIQERVFFLFDNLLVYCKRKHRRLKNSKASTDGHRYLFRGRI\nNTEVMEVENVDDGTADFHSSGHIVVNGWKIHNTAKNKWFVCMAKTPEEKHEWFEAILKER\nERRKGLKLGMEQDTWVMISEQGEKLYKMMCRQGNLIKDRKRKLTTFPKCFLGSEFVSWLL\nEIGEIHRPEEGVHLGQALLENGIIHHVTDKHQFKPEQMLYRFRYDDGTFYPRNEMQDVIS\nKGVRLYCRLHSLFTPVIRDKDYHLRTYKSVVMANKLIDWLIAQGDCRTREEAMIFGVGLC\nDNGFMHHVLEKSEFKDEPLLFRFFSDEEMEGSNMKHRLMKHDLKVVENVIAKSLLIKSNE\nGSYGFGLEDKNKVPIIKLVEKGSNAEMAGMEVGKKIFAINGDLVFMRPFNEVDCFLKSCL\nNSRKPLRVLVSTKPRETVKIPDSADGLGFQIRGFGPSVVHAVGRGTVAAAAGLHPGQCII\nKVNGINVSKETHASVIAHVTACRKYRRPTKQDSIQWVYNSIESAQEDLQKSHSKPPGDEA\nGDAFDCKVEEVIDKFNTMAIIDGKKEHVSLTVDNVHLEYGVVYEYDSTAGIKCNVVEKMI\nEPKGFFSLTAKILEALAKSDEHFVQNCTSLNSLNEVIPTDLQSKFSALCSERIEHLCQRI\nSSYKKFSRVLKNRAWPTFKQAKSKISPLHSSDFCPTNCHVNVMEVSYPKTSTSLGSAFGV\nQLDSRKHNSHDKENKSSEQGKLSPMVYIQHTITTMAAPSGLSLGQQDGHGLRYLLKEEDL\nETQDIYQKLLGKLQTALKEVEMCVCQIDDLLSSITYSPKLERKTSEGIIPTDSDNEKGER\nNSKRVCFNVAGDEQEDSGHDTISNRDSYSDCNSNRNSIASFTSICSSQCSSYFHSDEMDS\nGDELPLSVRISHDKQDKIHSCLEHLFSQVDSITNLLKGQAVVRAFDQTKYLTPGRGLQEF\nQQEMEPKLSCPKRLRLHIKQDPWNLPSSVRTLAQNIRKFVEEVKCRLLLALLEYSDSETQ\nLRRDMVFCQTLVATVCAFSEQLMAALNQMFDNSKENEMETWEASRRWLDQIANAGVLFHF\nQSLLSPNLTDEQAMLEDTLVALFDLEKVSFYFKPSEEEPLVANVPLTYQAEGSRQALKVY\nFYIDSYHFEQLPQRLKNGGGFKIHPVLFAQALESMEGYYYRDNVSVEEFQAQINAASLEK\nVKQYNQKLRAFYLDKSNSPPNSTSKAAYVDKLMRPLNALDELYRLVASFIRSKRTAACAN\nTACSASGVGLLSVSSELCNRLGACHIIMCSSGVHRCTLSVTLEQAIILARSHGLPPRYIM\nQATDVMRKQGARVQNTAKNLGVRDRTPQSAPRLYKLCEPPPPAGEE",
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"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": "Isolated P-Rex2 DH/PH tandem with residual linker (GAMDP) from N-terminal TEV cleavage"
}
],
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"pka": null,
"ph": 7.0,
"deuteration": null
},
"purity_method": null,
"name": "Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger protein 2 DH/PH domain tandem (P-Rex2 DH/PH)",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": null,
"full_name": "University of California, Davis",
"webpage": null
}
],
"contributor_name": "Lauren",
"contributor_surname": "Anderson",
"orcid": "http://lauanderson@ucdavis.edu"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2025-06-10",
"storage_temperature": null,
"cell_temperature": null,
"exposure_time": 2.0,
"number_of_frames": 750,
"wavelength": 0.1127,
"sample_detector_distance": 2.1,
"concentration_min": null,
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"flow_rate": 0.65,
"s_min": 0.124,
"s_max": 4.707,
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},
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{
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"software": "BILBOMD",
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"model_title": null,
"type_of_model": "atomic",
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"bead_radius": null,
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"comment": null,
"user": 1310
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDZV5_fit1_fitfoxs_img.png",
"software": "MultiFoXS",
"chi_square_value": 0.679376,
"p_value": 0.0017,
"fit_residual_plot": "SASDZV5_fit1_fitfoxsresiduals_img.png",
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"pddf_software": "ATSAS GNOM",
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"description": "Small-angle X-ray scattering (SAXS) experiments were performed at the SIBYLS beamline 12.3.1 at the Advanced Light Source. This beamline has additional inline instrumentation and detectors coupled to a size exclusion column. Purified P-Rex2 DH/PH was concentrated to 2 mg/ml and then buffer exchanged into 20mM HEPES (pH 7), 200mM NaCl, 2mM DTT, and 1% glycerol SEC running buffer. The X-ray wavelength was set to 1.127 Å and the sample-to-detector distance to 2,100 mm, which gives scattering vectors (q) ranging from 0.01 Å-1 to 0.4 Å-1. The scattering vector is q = 4πsinθ/λ, where 2θ is the scattering angle. The SAXS flow cell was coupled to an Agilent 1260 Infinity HPLC system using a Shodex PROTEIN KW-802.5 SEC column equilibrated with the running buffer with a flow rate of 0.65 ml/min. For SAXS measurements, 2 sec X-ray exposures were collected continuously during a 25 min elution. All frames for analyses had one SAXS frame corresponding to the running buffer before the detection of a peak subtracted from each.",
"experiment_description": null,
"tags": [],
"intensity_unit": "arbitrary",
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"porod_mw": 43.4,
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"pddf_i0": 9.84,
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"pddf_rg": 2.694,
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"pddf_dmax": 8.4,
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"porod_volume": 52.3,
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"estimated_volume": null,
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"guinier_point_first": 11,
"guinier_point_last": 76,
"pddf_point_first": null,
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"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDZV5_datloglog_img.png",
"symmetry": null,
"last_modified": "2026-07-23T20:42:10.836675+02:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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