Chloroplast Glutamyl Peptidase D855N in open-closed conformation

Method: ELECTRON MICROSCOPY D_max: 146.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	10et
deposition_date	2026-01-15
title	Chloroplast Glutamyl Peptidase D855N in open-closed conformation
keywords	S9 protease, enzyme, serine protease, alpha-beta-alpha sandwich fold, beta-propeller, PLANT PROTEIN; PLANT PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	43.53 Å
rg_electron	43.20 Å
i0	474688000.00
molecular_weight	179360.0 kDa
excluded_volume	224850 Å³
envelope_volume	345420 Å³
shell_volume	67650 Å³
envelope_diameter	157.0 Å
shell_rg	48.13 Å
envelope_rg	41.79 Å
shape_rg	43.18 Å
total_rg	43.53 Å
total_atoms	12659
n_residues	1604
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	146.3 Å
rg_real	43.58 Å
rg_real_error	1.72 Å
i0_real	4.7470e+08
i0_real_error	9.0540e+06
rg_reciprocal	43.53 Å
i0_reciprocal	474700000.0000
total_estimate	0.8711
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	48.8 Å
skewness	0.384
kurtosis	-0.351
angular_range	— – 0.1800 Å⁻¹
current_alpha	0.0000
highest_alpha	44290000.0000
n_real_points	37
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.827; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.842

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4. Crystallography & Experiment

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5. Entities & Polymer Info (1)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.000 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.00 Å
resolution_method	—
num_particles	70609

description	Isoform 2 of Probable glutamyl endopeptidase, chloroplastic
formula_weight	100357.6 kDa
ec_number	3.4.21.-
mutation	D855N
src_method	man
entity_count	2

Chloroplast Glutamyl Peptidase D855N in open-closed conformation

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (1)

Entity 1 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Arabidopsis thaliana
common_name	thale cress
ncbi_taxonomy_id	3702
host_organism	Escherichia coli BL21

fit	/app/data/saxs_computation/0e/10et/10et.fit
pddf	/app/data/pr_computation/0e/10et/10et.pr.out
profile	/app/data/saxs_computation/0e/10et/10et.dat

type	polypeptide(L)
strand_id	B,A
atom_count	12659