Chloroplast Glutamyl Peptidase D855N in open-open conformation

Method: ELECTRON MICROSCOPY D_max: 140.4 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	10eu
deposition_date	2026-01-15
title	Chloroplast Glutamyl Peptidase D855N in open-open conformation
keywords	S9 protease, enzyme, serine protease, alpha-beta-alpha sandwich fold, beta-propeller, PLANT PROTEIN; PLANT PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	44.07 Å
rg_electron	43.68 Å
i0	469370000.00
molecular_weight	178320.0 kDa
excluded_volume	223570 Å³
envelope_volume	348650 Å³
shell_volume	67539 Å³
envelope_diameter	156.6 Å
shell_rg	48.31 Å
envelope_rg	42.05 Å
shape_rg	43.65 Å
total_rg	44.01 Å
total_atoms	12586
n_residues	1594
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	140.4 Å
rg_real	44.06 Å
rg_real_error	1.06 Å
i0_real	4.6940e+08
i0_real_error	8.1330e+06
rg_reciprocal	44.08 Å
i0_reciprocal	469400000.0000
total_estimate	0.5958
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	50.7 Å
skewness	0.324
kurtosis	-0.362
angular_range	— – 0.1800 Å⁻¹
current_alpha	0.0000
highest_alpha	43360000.0000
n_real_points	37
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.910; Stabil: 0.999; Sysdev: 0.005; Positv: 1.000; Valcen: 0.999; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (1)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.000 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.00 Å
resolution_method	—
num_particles	55962

description	Isoform 2 of Probable glutamyl endopeptidase, chloroplastic
formula_weight	100357.6 kDa
ec_number	3.4.21.-
mutation	D855N
src_method	man
entity_count	2

Chloroplast Glutamyl Peptidase D855N in open-open conformation

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (1)

Entity 1 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Arabidopsis thaliana
common_name	thale cress
ncbi_taxonomy_id	3702
host_organism	Escherichia coli BL21

fit	/app/data/saxs_computation/0e/10eu/10eu.fit
pddf	/app/data/pr_computation/0e/10eu/10eu.pr.out
profile	/app/data/saxs_computation/0e/10eu/10eu.dat

type	polypeptide(L)
strand_id	A,B
atom_count	12586