10tx

Tissue Non-specific Alkaline Phosphatase -S110A bound to PPi

Method: X-RAY DIFFRACTION D_max: 175.8 Å Quality: SUSPICIOUS

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Alkaline phosphatase, tissue-nonspecific isozyme

Mus musculus

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Other combination Homooligomer Protein × 4 其他Polymer 9 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain A； UniProt 17–501 Chain B； UniProt 17–501 Chain C； UniProt 17–501 Chain D； UniProt 17–501	Mutation：S110A	;alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose ; × 2 beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 6 NA SODIUM ION × 4 POP PYROPHOSPHATE 2- × 4 ZN ZINC ION × 4 MG MAGNESIUM ION × 4 CA CALCIUM ION × 4 GOL GLYCEROL × 10 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 PO4 PHOSPHATE ION × 2 FLC CITRATE ANION × 1	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；293.15 K；0.15 M phosphate/citrate pH 4.0, 0.2 M NaCl, 0-30 mM phosphate, 5% glycerol, 14-16% PEG 10,000	Resolution 2.25 Å R-free 0.232

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 10TX	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
10TV Tissue Non-specific Alkaline Phosphatase -S110A bound to phosphocreatine Deposited 2026-02-09	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: octameric	Chain A 17–501（485 aa） Chain B 17–501（485 aa） Chain C 17–501（485 aa） Chain D 17–501（485 aa）	Mutation：S110A Mutation：S110A Mutation：S110A Mutation：S110A	XDC Phosphocreatine × 4 ZN ZINC ION × 4 MG MAGNESIUM ION × 4 CA CALCIUM ION × 4 GOL GLYCEROL × 10 NA SODIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 10 MAN alpha-D-mannopyranose × 1 PO4 PHOSPHATE ION × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 FLC CITRATE ANION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；0.15 M phosphate/citrate pH 4.0, 0.2 M NaCl, 0-30 mM phosphate, 5% glycerol, 14-16% PEG 10,000	Resolution 2.26 Å R-free 0.216
10TW Tissue Non-specific Alkaline Phosphatase -D370G bound to phosphocreatine Deposited 2026-02-09	Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 17–501（485 aa） Chain B 17–501（485 aa） Chain C 17–501（485 aa） Chain D 17–501（485 aa）	Mutation：D370G Mutation：D370G Mutation：D370G Mutation：D370G	PO4 PHOSPHATE ION × 4 ZN ZINC ION × 4 MG MAGNESIUM ION × 4 CA CALCIUM ION × 4 NA SODIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 9 GOL GLYCEROL × 6 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 FLC CITRATE ANION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；0.15 M phosphate/citrate pH 4.0, 0.2 M NaCl, 0-30 mM phosphate, 5% glycerol, 14-16% PEG 10,000	Resolution 2.12 Å R-free 0.216
10TY Tissue Non-specific Alkaline Phosphatase -S110A bound to PPi with ethylene glycol Deposited 2026-02-09	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 17–501（485 aa） Chain B 17–501（485 aa） Chain C 17–501（485 aa） Chain D 17–501（485 aa）	Mutation：S110A Mutation：S110A Mutation：S110A Mutation：S110A	POP PYROPHOSPHATE 2- × 4 ZN ZINC ION × 4 MG MAGNESIUM ION × 4 CA CALCIUM ION × 4 NA SODIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 9 MAN alpha-D-mannopyranose × 1 PO4 PHOSPHATE ION × 2 FLC CITRATE ANION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；0.15 M phosphate/citrate pH 4.0, 0.2 M NaCl, 0-30 mM phosphate, 14-16% PEG 10,000	Resolution 2.28 Å R-free 0.249
10TZ Tissue Non-specific Alkaline Phosphatase -S110A bound to ATP Deposited 2026-02-09	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 17–501（485 aa） Chain B 17–501（485 aa） Chain C 17–501（485 aa） Chain D 17–501（485 aa）	Mutation：S110A Mutation：S110A Mutation：S110A Mutation：S110A	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 MG MAGNESIUM ION × 3 CA CALCIUM ION × 4 GOL GLYCEROL × 10 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 FLC CITRATE ANION × 1 PO4 PHOSPHATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293.15 K；0.15 M phosphate/citrate pH 4.0, 0.2 M NaCl, 0-30 mM phosphate, 5% glycerol, 14-16% PEG 10,000	Resolution 2.71 Å R-free 0.310

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	PPBT_MOUSE
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 3–487； UniProt 17–501 Author chain B； PDBConstruct 3–487； UniProt 17–501 Author chain C； PDBConstruct 3–487； UniProt 17–501 Author chain D； PDBConstruct 3–487； UniProt 17–501

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	10tx
Deposition date deposition_date	2026-02-09
Structure title title	Tissue Non-specific Alkaline Phosphatase -S110A bound to PPi
Keywords keywords	TNAP, HYDROLASE; HYDROLASE
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	52.16 Å
Radius of gyration Rg (electron density) rg_electron	52.89 Å
Forward intensity I(0) i0	760173000.00
Molecular weight molecular_weight	220340.0 kDa
Excluded volume excluded_volume	271930 Å³
Envelope volume envelope_volume	352980 Å³
Hydration-shell volume shell_volume	59180 Å³
Envelope diameter envelope_diameter	192.0 Å
Shell Rg shell_rg	49.98 Å
Envelope Rg envelope_rg	52.92 Å
Shape Rg shape_rg	52.89 Å
Total Rg total_rg	52.80 Å
Total atoms total_atoms	15433
Residues n_residues	1919
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	175.8 Å
Rg (real space) rg_real	52.77 Å
Rg uncertainty (real space) rg_real_error	1.97 Å
I(0) (real space) i0_real	7.6020e+08
I(0) uncertainty (real space) i0_real_error	1.3610e+07
Rg (reciprocal space) rg_reciprocal	51.63 Å
I(0) (reciprocal space) i0_reciprocal	759000000.0000
Solution quality estimate total_estimate	0.4822
Solution quality rating solution_quality	SUSPICIOUS a SUSPICIOUS solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	35.1 Å
Skewness Skewness skewness	0.569
Kurtosis Kurtosis kurtosis	-0.524
Angular range angular_range	— – 0.1500 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	106100000.0000
Real-space data points n_real_points	31
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.475; Stabil: 1.000; Sysdev: 0.007; Positv: 1.000; Valcen: 0.664; Smooth: 0.153

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (14)

Entity 1 Entity 1 polymer

Description description	Alkaline phosphatase, tissue-nonspecific isozyme
Molecular weight formula_weight	54634.3 kDa
EC 编号 ec_number	3.1.3.1,3.9.1.1
Mutation mutation	S110A
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D
Atom count atom_count	16274

DGSFVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDAAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAGHHHHHH

来源 Source

来源生物 organism	Mus musculus
通用名 common_name	house mouse
NCBI Class编号 ncbi_taxonomy_id	10090
宿主生物 host_organism	Spodoptera frugiperda

Entity 2 Entity 2 branched

Description description	;alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose ;
Molecular weight formula_weight	1057.0 kDa
Source method src_method	man
Copy count entity_count	2

Entity 3 Entity 3 branched

Description description	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
Molecular weight formula_weight	586.5 kDa
Source method src_method	man
Copy count entity_count	1

Entity 4 Entity 4 branched

Description description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
Molecular weight formula_weight	424.4 kDa
Source method src_method	man
Copy count entity_count	6

Entity 5 Entity 5 non-polymer

Description description	SODIUM ION
Molecular weight formula_weight	23.0 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 6 Entity 6 non-polymer

Description description	PYROPHOSPHATE 2-
Molecular weight formula_weight	176.0 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 7 Entity 7 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 8 Entity 8 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 9 Entity 9 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 10 Entity 10 non-polymer

Description description	GLYCEROL
Molecular weight formula_weight	92.1 kDa
Source method src_method	syn
Copy count entity_count	10

Entity 11 Entity 11 non-polymer

Description description	2-acetamido-2-deoxy-beta-D-glucopyranose
Molecular weight formula_weight	221.2 kDa
Source method src_method	nat
Copy count entity_count	6

Entity 12 Entity 12 non-polymer

Description description	PHOSPHATE ION
Molecular weight formula_weight	95.0 kDa
Source method src_method	nat
Copy count entity_count	2

Entity 13 Entity 13 non-polymer

Description description	CITRATE ANION
Molecular weight formula_weight	189.1 kDa
Source method src_method	nat
Copy count entity_count	1

Entity 14 Entity 14 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	797

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8. Citations (1)

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Unit-cell length a length_a	66.305 Å
Unit-cell length b length_b	118.893 Å
Unit-cell length c length_c	346.483 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	orthorhombic

Resolution resolution	2.250 Å
Low-resolution limit resolution_low	39.660 Å
R factor r_factor	0.1909
R_work r_work	0.1903
R_free r_free	0.2318

fit	/app/data/saxs_computation/0t/10tx/10tx.fit
pddf	/app/data/pr_computation/0t/10tx/10tx.pr.out
profile	/app/data/saxs_computation/0t/10tx/10tx.dat