SAXSdb — 13hs

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1. Protein Identity and Related Structures Protein Identity & Related Structures

ABC-type transport auxiliary lipoprotein component domain-containing protein

Pseudomonas aeruginosa PAO1

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein homooligomer Homooligomer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count	Chain A； UniProt 1–214 Chain B； UniProt 1–214 Chain C； UniProt 1–214 Chain D； UniProt 1–214 Chain E； UniProt 1–214 Chain F； UniProt 1–214 Chain G； UniProt 1–214 Chain H； UniProt 1–214	Not recorded	No other associated polymer	ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 3.70 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 13HS	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
13HQ Cryo-EM structure of Pseudomonas aeruginosa soluble lipoprotein PA3214 Deposited 2026-05-06	Different construct Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric	Chain A 27–214（188 aa） Chain B 27–214（188 aa） Chain C 27–214（188 aa） Chain D 27–214（188 aa） Chain E 27–214（188 aa） Chain F 27–214（188 aa） Chain G 27–214（188 aa） Chain H 27–214（188 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.67 Å
13HR Cryo-EM structure of Pseudomonas aeruginosa outer-membrane lipoprotein PA3214 bound to MCE protein PA3213 C-terminal peptide Deposited 2026-05-06	Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: 16-meric	Chain A 1–214（214 aa） Chain B 1–214（214 aa） Chain C 1–214（214 aa） Chain D 1–214（214 aa） Chain E 1–214（214 aa） Chain F 1–214（214 aa） Chain G 1–214（214 aa） Chain H 1–214（214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	Q9HZ26_PSEAE
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–214； UniProt 1–214 Author chain B； PDBConstruct 1–214； UniProt 1–214 Author chain C； PDBConstruct 1–214； UniProt 1–214 Author chain D； PDBConstruct 1–214； UniProt 1–214 Author chain E； PDBConstruct 1–214； UniProt 1–214 Author chain F； PDBConstruct 1–214； UniProt 1–214 Author chain G； PDBConstruct 1–214； UniProt 1–214 Author chain H； PDBConstruct 1–214； UniProt 1–214

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	13hs
Deposition date deposition_date	2026-05-06
Structure title title	Cryo-EM structure of Pseudomonas aeruginosa outer-membrane lipoprotein PA3214 in the open conformation
Keywords keywords	outer membrane lipoprotein, Pseudomonas aeruginosa, MCE system, LIPID TRANSPORT; LIPID TRANSPORT
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	42.41 Å
Radius of gyration Rg (electron density) rg_electron	41.61 Å
Forward intensity I(0) i0	385847000.00
Molecular weight molecular_weight	156260.0 kDa
Excluded volume excluded_volume	194190 Å³
Envelope volume envelope_volume	311970 Å³
Hydration-shell volume shell_volume	60649 Å³
Envelope diameter envelope_diameter	122.3 Å
Shell Rg shell_rg	50.53 Å
Envelope Rg envelope_rg	38.98 Å
Shape Rg shape_rg	41.63 Å
Total Rg total_rg	42.05 Å
Total atoms total_atoms	11040
Residues n_residues	1424
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	122.9 Å
Rg (real space) rg_real	42.14 Å
Rg uncertainty (real space) rg_real_error	1.02 Å
I(0) (real space) i0_real	3.8580e+08
I(0) uncertainty (real space) i0_real_error	6.1270e+06
Rg (reciprocal space) rg_reciprocal	42.41 Å
I(0) (reciprocal space) i0_reciprocal	386000000.0000
Solution quality estimate total_estimate	0.8870
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	69.8 Å
Skewness Skewness skewness	-0.143
Kurtosis Kurtosis kurtosis	-0.886
Angular range angular_range	— – 0.1850 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	85880000.0000
Real-space data points n_real_points	38
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.867; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.928

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.700 Å
Low-resolution limit resolution_low	— Å
R factor r_factor	—
R_work r_work	—
R_free r_free	—

Resolution resolution	3.70 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	187311

Description description	ABC-type transport auxiliary lipoprotein component domain-containing protein
Molecular weight formula_weight	23243.5 kDa
Source method src_method	man
Copy count entity_count	8

Cryo-EM structure of Pseudomonas aeruginosa outer-membrane lipoprotein PA3214 in the open conformation

1. Protein Identity and Related Structures Protein Identity & Related Structures

ABC-type transport auxiliary lipoprotein component domain-containing protein

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

冷冻电镜 EM 3D

6. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G,H
Atom count atom_count	11040

来源生物 organism	Pseudomonas aeruginosa PAO1
NCBI Class编号 ncbi_taxonomy_id	208964
宿主生物 host_organism	Escherichia coli BL21(DE3)

fit	/app/data/saxs_computation/3h/13hs/13hs.fit
pddf	/app/data/pr_computation/3h/13hs/13hs.pr.out
profile	/app/data/saxs_computation/3h/13hs/13hs.dat