← Back to search

1a9y

UDP-GALACTOSE 4-EPIMERASE MUTANT S124A/Y149F COMPLEXED WITH UDP-GLUCOSE

Method: X-RAY DIFFRACTION D_max: 61.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1a9y
deposition_date	1998-04-14
title	UDP-GALACTOSE 4-EPIMERASE MUTANT S124A/Y149F COMPLEXED WITH UDP-GLUCOSE
keywords	EPIMERASE, GALACTOSE METABOLISM, DEHYDROGENASE; EPIMERASE
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	19.91 Å
rg_electron	18.97 Å
i0	26551200.00
molecular_weight	38547.0 kDa
excluded_volume	47705 Å³
envelope_volume	52126 Å³
shell_volume	22347 Å³
envelope_diameter	62.3 Å
shell_rg	25.96 Å
envelope_rg	19.14 Å
shape_rg	18.98 Å
total_rg	19.80 Å
total_atoms	2708
n_residues	338
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	61.8 Å
rg_real	19.77 Å
rg_real_error	0.28 Å
i0_real	2.6550e+07
i0_real_error	3.1360e+05
rg_reciprocal	19.79 Å
i0_reciprocal	26550000.0000
total_estimate	0.8955
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	25.2 Å
skewness	0.183
kurtosis	-0.348
angular_range	— – 0.4000 Å⁻¹
current_alpha	0.0000
highest_alpha	7035000.0000
n_real_points	72
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.889; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.991; Smooth: 0.982

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (5)

Entity 1 polymer

description	UDP-GALACTOSE 4-EPIMERASE
formula_weight	37276.0 kDa
ec_number	5.1.3.2
mutation	S124A, Y149F
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	3249

MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVIERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVNGTLRLISAMRAANVKNFIFSSAATVYGDQPKIPYVESFPTGTPQSPFGKSKLMVEQILTDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIFGNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAFSKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQGYPD

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

Entity 2 non-polymer

description	SODIUM ION
formula_weight	23.0 kDa
src_method	syn
entity_count	4

Entity 3 non-polymer

description	NICOTINAMIDE-ADENINE-DINUCLEOTIDE
formula_weight	663.4 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	URIDINE-5'-DIPHOSPHATE-GLUCOSE
formula_weight	566.3 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	541

▶

6. Citations (1)

▶

length_a	85.000 Å
length_b	85.000 Å
length_c	106.700 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 32 2 1
int_tables_number	154
crystal_system	—

resolution	1.800 Å
resolution_low	30.000 Å
r_factor	0.1810
r_work	0.1810
r_free	—

fit	/app/data/saxs_computation/a9/1a9y/1a9y.fit
pddf	/app/data/pr_computation/a9/1a9y/1a9y.pr.out
profile	/app/data/saxs_computation/a9/1a9y/1a9y.dat