1b25

FORMALDEHYDE FERREDOXIN OXIDOREDUCTASE FROM PYROCOCCUS FURIOSUS

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN (FORMALDEHYDE FERREDOXIN OXIDOREDUCTASE)

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 4	IRON/SULFUR CLUSTER × 4 Oxo-tungstopterin cofactor × 4 MAGNESIUM ION × 4 CALCIUM ION × 4 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	O93738_PYRFU
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–619； UniProt 1–619

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1b25
Deposition date deposition_date	1998-12-04
Structure title title	FORMALDEHYDE FERREDOXIN OXIDOREDUCTASE FROM PYROCOCCUS FURIOSUS
Keywords keywords	MOCO, TUNGSTOENZYME, TUNGSTEN CONTAINING PROTEIN, HYPERTHERMOPHILE, OXIDOREDUCTASE; OXIDOREDUCTASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1b25__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1b25__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1b25__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	46.11 Å
Rg (electron density)	45.97 Å
Total Rg	45.89 Å
Atom count	19384
Residues	2444
Excluded volume	346640 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1b25__assembly_1__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Description description	PROTEIN (FORMALDEHYDE FERREDOXIN OXIDOREDUCTASE)
Molecular weight formula_weight	68860.7 kDa
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D
Atom count atom_count	20733

MYGWWGRILRVNLTTGEVKVQEYPEEVAKKFIGGRGLAAWILWNEARGVEPLSPENKLIFAAGPFNGLPTPSGGKLVVAAKSPLTGGYGDGNLGTMASVHLRRAGYDALVVEGKAKKPVYIYIEDDNVSILSAEGLWGKTTFETERELKEIHGKNVGVLTIGPAGENLVKYAVVISQEGRAAGRPGMGAVMGSKKLKAVVIRGTKEIPVADKEELKKLSQEAYNEILNSPGYPFWKRQGTMAAVEWCNTNYALPTRNFSDGYFEFARSIDGYTMEGMKVQQRGCPYCNMPCGNVVLDAEGQESELDYENVALLGSNLGIGKLNEVSVLNRIADEMGMDTISLGVSIAHVMEAVERGILKEGPTFGDFKGAKQLALDIAYRKGELGNLAAEGVKAMAEKLGTHDFAMHVKGLEVSGYNCYIYPAMALAYGTSAIGAHHKEAWVIAWEIGTAPIEGEKAEKVEYKISYDPIKAQKVVELQRLRGGLFEMLTACRLPWVEVGLSLDYYPKLLKAITGVTYTWDDLYKAADRVYSLIRAYWVREFNGKWDRKMDYPPKRWFTEGLKSGPHKGEHLDEKKYDELLSEYYRIRGWDERGIPKKETLKELDLDFVIPELEKVTNLE

Entity 2 Entity 2 non-polymer

Description description	IRON/SULFUR CLUSTER
Molecular weight formula_weight	351.6 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 3 Entity 3 non-polymer

Description description	Oxo-tungstopterin cofactor
Molecular weight formula_weight	987.5 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 4 Entity 4 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	nat
Copy count entity_count	4

Entity 5 Entity 5 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	nat
Copy count entity_count	4

Entity 6 Entity 6 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	1349

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6. Fold Classification (SCOP + CATH) 20 domains

SCOP 2.08 (8 domains)

Domain ID domain_id	d1b25a1
Class class	a — All alpha proteins
Fold Fold fold	a.110 — Aldehyde ferredoxin oxidoreductase, C-terminal domains
Superfamily Superfamily superfamily	a.110.1 — Aldehyde ferredoxin oxidoreductase, C-terminal domains
Family Family family	a.110.1.1 — Aldehyde ferredoxin oxidoreductase, C-terminal domains

Domain ID domain_id	d1b25a2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.152 — Aldehyde ferredoxin oxidoreductase, N-terminal domain
Superfamily Superfamily superfamily	d.152.1 — Aldehyde ferredoxin oxidoreductase, N-terminal domain
Family Family family	d.152.1.1 — Aldehyde ferredoxin oxidoreductase, N-terminal domain

Domain ID domain_id	d1b25b1
Class class	a — All alpha proteins
Fold Fold fold	a.110 — Aldehyde ferredoxin oxidoreductase, C-terminal domains
Superfamily Superfamily superfamily	a.110.1 — Aldehyde ferredoxin oxidoreductase, C-terminal domains
Family Family family	a.110.1.1 — Aldehyde ferredoxin oxidoreductase, C-terminal domains

Domain ID domain_id	d1b25b2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.152 — Aldehyde ferredoxin oxidoreductase, N-terminal domain
Superfamily Superfamily superfamily	d.152.1 — Aldehyde ferredoxin oxidoreductase, N-terminal domain
Family Family family	d.152.1.1 — Aldehyde ferredoxin oxidoreductase, N-terminal domain

Domain ID domain_id	d1b25c1
Class class	a — All alpha proteins
Fold Fold fold	a.110 — Aldehyde ferredoxin oxidoreductase, C-terminal domains
Superfamily Superfamily superfamily	a.110.1 — Aldehyde ferredoxin oxidoreductase, C-terminal domains
Family Family family	a.110.1.1 — Aldehyde ferredoxin oxidoreductase, C-terminal domains

Domain ID domain_id	d1b25c2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.152 — Aldehyde ferredoxin oxidoreductase, N-terminal domain
Superfamily Superfamily superfamily	d.152.1 — Aldehyde ferredoxin oxidoreductase, N-terminal domain
Family Family family	d.152.1.1 — Aldehyde ferredoxin oxidoreductase, N-terminal domain

Domain ID domain_id	d1b25d1
Class class	a — All alpha proteins
Fold Fold fold	a.110 — Aldehyde ferredoxin oxidoreductase, C-terminal domains
Superfamily Superfamily superfamily	a.110.1 — Aldehyde ferredoxin oxidoreductase, C-terminal domains
Family Family family	a.110.1.1 — Aldehyde ferredoxin oxidoreductase, C-terminal domains

Domain ID domain_id	d1b25d2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.152 — Aldehyde ferredoxin oxidoreductase, N-terminal domain
Superfamily Superfamily superfamily	d.152.1 — Aldehyde ferredoxin oxidoreductase, N-terminal domain
Family Family family	d.152.1.1 — Aldehyde ferredoxin oxidoreductase, N-terminal domain

CATH v4.4 (12 domains)

Domain ID domain_id	1b25A01
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	9 — Aldehyde Ferredoxin Oxidoreductase; A, domain 1
Homologous superfamily homologous superfamily	10 — Aldehyde ferredoxin oxidoreductase, N-terminal domain

Domain ID domain_id	1b25A02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	569 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A; domain 2
Homologous superfamily homologous superfamily	10 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2

Domain ID domain_id	1b25A03
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	599 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A; domain 3
Homologous superfamily homologous superfamily	10 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3

Domain ID domain_id	1b25B01
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	9 — Aldehyde Ferredoxin Oxidoreductase; A, domain 1
Homologous superfamily homologous superfamily	10 — Aldehyde ferredoxin oxidoreductase, N-terminal domain

Domain ID domain_id	1b25B02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	569 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A; domain 2
Homologous superfamily homologous superfamily	10 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2

Domain ID domain_id	1b25B03
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	599 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A; domain 3
Homologous superfamily homologous superfamily	10 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3

Domain ID domain_id	1b25C01
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	9 — Aldehyde Ferredoxin Oxidoreductase; A, domain 1
Homologous superfamily homologous superfamily	10 — Aldehyde ferredoxin oxidoreductase, N-terminal domain

Domain ID domain_id	1b25C02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	569 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A; domain 2
Homologous superfamily homologous superfamily	10 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2

Domain ID domain_id	1b25C03
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	599 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A; domain 3
Homologous superfamily homologous superfamily	10 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3

Domain ID domain_id	1b25D01
Class class	3 — Alpha Beta
Architecture architecture	60 — 4-Layer Sandwich
Topology topology	9 — Aldehyde Ferredoxin Oxidoreductase; A, domain 1
Homologous superfamily homologous superfamily	10 — Aldehyde ferredoxin oxidoreductase, N-terminal domain

Domain ID domain_id	1b25D02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	569 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A; domain 2
Homologous superfamily homologous superfamily	10 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2

Domain ID domain_id	1b25D03
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	599 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A; domain 3
Homologous superfamily homologous superfamily	10 — Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3

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Unit-cell length a length_a	100.340 Å
Unit-cell length b length_b	170.850 Å
Unit-cell length c length_c	180.640 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	—

Resolution resolution	1.850 Å
Low-resolution limit resolution_low	20.000 Å
R factor r_factor	0.1740
R_work r_work	0.1740
R_free r_free	0.2200

FORMALDEHYDE FERREDOXIN OXIDOREDUCTASE FROM PYROCOCCUS FURIOSUS

1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN (FORMALDEHYDE FERREDOXIN OXIDOREDUCTASE)

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1b25__assembly_1__model_1

1b25__assembly_1__model_1 | I(q)

1b25__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 water

6. Fold Classification (SCOP + CATH) 20 domains

SCOP 2.08 (8 domains)

CATH v4.4 (12 domains)

7. Citations (2)

FORMALDEHYDE FERREDOXIN OXIDOREDUCTASE FROM PYROCOCCUS FURIOSUS

1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN (FORMALDEHYDE FERREDOXIN OXIDOREDUCTASE)

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1b25__assembly_1__model_1

1b25__assembly_1__model_1 | I(q)

1b25__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 water

6. Fold Classification (SCOP + CATH) 20 domains

SCOP 2.08 (8 domains)

CATH v4.4 (12 domains)

7. Citations (2)