THERMOTOGA MARITIMA GLUTAMATE DEHYDROGENASE MUTANT N97D, G376K

Method: X-RAY DIFFRACTION D_max: 135.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1b3b
deposition_date	1998-12-07
title	THERMOTOGA MARITIMA GLUTAMATE DEHYDROGENASE MUTANT N97D, G376K
keywords	METABOLIC ROLE, GLUTAMATE, DEHYDROGENASE, MUTANT, OXIDOREDUCTASE; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	42.53 Å
rg_electron	41.89 Å
i0	1047110000.00
molecular_weight	269960.0 kDa
excluded_volume	339570 Å³
envelope_volume	439270 Å³
shell_volume	84282 Å³
envelope_diameter	139.9 Å
shell_rg	48.93 Å
envelope_rg	41.77 Å
shape_rg	41.86 Å
total_rg	42.33 Å
total_atoms	19008
n_residues	2454
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	135.7 Å
rg_real	42.34 Å
rg_real_error	0.90 Å
i0_real	1.0470e+09
i0_real_error	1.7890e+07
rg_reciprocal	42.53 Å
i0_reciprocal	1047000000.0000
total_estimate	0.8774
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	55.0 Å
skewness	0.191
kurtosis	-0.354
angular_range	— – 0.1850 Å⁻¹
current_alpha	0.0000
highest_alpha	150000000.0000
n_real_points	38
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.870; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.972; Smooth: 0.821

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4. Crystallography & Experiment

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5. Entities & Polymer Info (1)

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6. Citations (1)

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length_a	145.100 Å
length_b	145.100 Å
length_c	272.500 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	36

space_group	P 31 2 1
int_tables_number	152
crystal_system	—

resolution	3.100 Å
resolution_low	8.000 Å
r_factor	0.2250
r_work	0.2250
r_free	0.2980

description	PROTEIN (GLUTAMATE DEHYDROGENASE)
formula_weight	45801.4 kDa
ec_number	1.4.1.3
mutation	N97D, G376K
src_method	man
entity_count	6

type	polypeptide(L)
strand_id	A,B,C,D,E,F
atom_count	19008

THERMOTOGA MARITIMA GLUTAMATE DEHYDROGENASE MUTANT N97D, G376K

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (1)

Entity 1 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Thermotoga maritima
ncbi_taxonomy_id	2336
host_organism	Escherichia coli BL21(DE3)

fit	/app/data/saxs_computation/b3/1b3b/1b3b.fit
pddf	/app/data/pr_computation/b3/1b3b/1b3b.pr.out
profile	/app/data/saxs_computation/b3/1b3b/1b3b.dat