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1b80

REC. LIGNIN PEROXIDASE H8 OXIDATIVELY PROCESSED

Method: X-RAY DIFFRACTION D_max: 123.2 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1b80
deposition_date	1999-02-03
title	REC. LIGNIN PEROXIDASE H8 OXIDATIVELY PROCESSED
keywords	;LIGNIN DEGRADATION, HEME, RADICAL REACTION, ELECTRON TRANSFER, AUTOCATALYTIC SELF-OXIDATION, BETA-HYDROXY TRYPTOPHAN, OXIDOREDUCTASE ;; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	35.36 Å
rg_electron	35.44 Å
i0	90400400.00
molecular_weight	75531.0 kDa
excluded_volume	93833 Å³
envelope_volume	116740 Å³
shell_volume	29291 Å³
envelope_diameter	125.7 Å
shell_rg	38.89 Å
envelope_rg	35.26 Å
shape_rg	35.43 Å
total_rg	35.73 Å
total_atoms	5315
n_residues	692
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	123.2 Å
rg_real	35.81 Å
rg_real_error	1.13 Å
i0_real	9.0400e+07
i0_real_error	1.6810e+06
rg_reciprocal	35.53 Å
i0_reciprocal	90380000.0000
total_estimate	0.7269
solution_quality	REASONABLE a REASONABLE solution
n_peaks	1
r_peak_primary	27.4 Å
skewness	0.527
kurtosis	-0.615
angular_range	— – 0.2250 Å⁻¹
current_alpha	0.0000
highest_alpha	23430000.0000
n_real_points	46
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.408; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.392; Smooth: 0.828

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	PROTEIN (RECOMBINANT LIGNIN PEROXIDASE H8)
formula_weight	37496.7 kDa
ec_number	1.11.1.14
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	5892

AAVIEKRATCSNGKTVGDASCCAWFDVLDDIQQNLFHGGQCGAEAHESIRLVFHDSIAISPAMEAQGKFGGGGADGSIMIFDDIETAFHPNIGLDEIVKLQKPFVQKHGVTPGDFIAFAGAVALSNCPGAPQMNFFTGRAPATQPAPDGLVPEPFHTVDQIINRVNDAGEFDELELVWMLSAHSVAAVNDVDPTVQGLPFDSTPGIFDSQFFVETQLRGTAFPGSGGNQGEVESPLPGEIRIQSDHTIARDSRTACEWQSFVNNQSKLVDDFQFIFLALTQLGQDPNAMTDCSDVIPQSKPIPGNLPFSFFPAGKTIKDVEQACAETPFPTLTTLPGPETSVQRIPPPPGA

Source

organism	Phanerochaete chrysosporium
ncbi_taxonomy_id	5306
host_organism	Escherichia coli

Entity 2 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	4

Entity 3 non-polymer

description	PROTOPORPHYRIN IX CONTAINING FE
formula_weight	616.5 kDa
src_method	syn
entity_count	2

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	573

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6. Citations (4)

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length_a	73.480 Å
length_b	94.930 Å
length_c	230.130 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	C 2 2 21
int_tables_number	20
crystal_system	—

resolution	1.730 Å
resolution_low	20.000 Å
r_factor	—
r_work	0.1700
r_free	0.2080

fit	/app/data/saxs_computation/b8/1b80/1b80.fit
pddf	/app/data/pr_computation/b8/1b80/1b80.pr.out
profile	/app/data/saxs_computation/b8/1b80/1b80.dat

REC. LIGNIN PEROXIDASE H8 OXIDATIVELY PROCESSED

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction