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1bc3

RECOMBINANT RAT ANNEXIN V, TRIPLE MUTANT (T72K, S144K, S228K)

Method: X-RAY DIFFRACTION D_max: 77.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1bc3
deposition_date	1998-05-04
title	RECOMBINANT RAT ANNEXIN V, TRIPLE MUTANT (T72K, S144K, S228K)
keywords	CALCIUM BINDING PROTEIN, PHOSPHOLIPID MEMBRANE BINDING PROTEIN, CALCIUM-BINDING PROTEIN; CALCIUM-BINDING PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	22.74 Å
rg_electron	21.84 Å
i0	22251500.00
molecular_weight	35976.0 kDa
excluded_volume	45071 Å³
envelope_volume	52737 Å³
shell_volume	21032 Å³
envelope_diameter	78.5 Å
shell_rg	27.90 Å
envelope_rg	22.06 Å
shape_rg	21.82 Å
total_rg	22.69 Å
total_atoms	2520
n_residues	318
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	77.3 Å
rg_real	22.79 Å
rg_real_error	0.54 Å
i0_real	2.2250e+07
i0_real_error	2.9260e+05
rg_reciprocal	22.78 Å
i0_reciprocal	22250000.0000
total_estimate	0.8688
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	24.6 Å
skewness	0.463
kurtosis	-0.080
angular_range	— – 0.3500 Å⁻¹
current_alpha	0.0000
highest_alpha	5385000.0000
n_real_points	67
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.785; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.973; Smooth: 0.962

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	ANNEXIN V
formula_weight	35900.7 kDa
mutation	T72K, S144K, S228K
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2629

MALRGTVTDFSGFDGRADAEVLRKAMKGLGTDEDSILNLLTARSNAQRQQIAEEFKTLFGRDLVNDMKSELKGKFEKLIVALMKPSRLYDAYELKHALKGAGTDEKVLTEIIASRTPEELRAIKQAYEEEYGSNLEDDVVGDTKGYYQRMLVVLLQANRDPDTAIDDAQVELDAQALFQAGELKWGTDEEKFITILGTRSVSHLRRVFDKYMTISGFQIEETIDRETKGNLENLLLAVVKSIRSIPAYLAETLYYAMKGAGTDDHTLIRVIVSRSEIDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGGEDD

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli

Entity 2 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	5

Entity 3 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	109

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6. Citations (1)

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length_a	157.340 Å
length_b	157.340 Å
length_c	37.590 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	9

space_group	H 3
int_tables_number	146
crystal_system	—

resolution	1.950 Å
resolution_low	10.000 Å
r_factor	0.2590
r_work	0.2590
r_free	0.2110

fit	/app/data/saxs_computation/bc/1bc3/1bc3.fit
pddf	/app/data/pr_computation/bc/1bc3/1bc3.pr.out
profile	/app/data/saxs_computation/bc/1bc3/1bc3.dat

RECOMBINANT RAT ANNEXIN V, TRIPLE MUTANT (T72K, S144K, S228K)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction