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1bf4

CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX

Method: X-RAY DIFFRACTION D_max: 47.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1bf4
deposition_date	1998-05-27
title	CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX
keywords	DNA BINDING PROTEIN, HYPERTHERMOPHILE, ARCHAEBACTERIA, COMPLEX (DNA-BINDING PROTEIN-DNA), DNA-BINDING PROTEIN-DNA complex; DNA-BINDING PROTEIN/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	14.10 Å
rg_electron	13.14 Å
i0	4466410.00
molecular_weight	12100.0 kDa
excluded_volume	13801 Å³
envelope_volume	16057 Å³
shell_volume	10616 Å³
envelope_diameter	46.8 Å
shell_rg	18.53 Å
envelope_rg	13.46 Å
shape_rg	13.07 Å
total_rg	14.24 Å
total_atoms	824
n_residues	78
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	47.2 Å
rg_real	14.02 Å
rg_real_error	0.35 Å
i0_real	4.4660e+06
i0_real_error	5.3990e+04
rg_reciprocal	14.03 Å
i0_reciprocal	4466000.0000
total_estimate	0.8748
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	18.5 Å
skewness	0.192
kurtosis	-0.298
angular_range	— – 0.5000 Å⁻¹
current_alpha	0.0000
highest_alpha	470200.0000
n_real_points	80
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.794; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.988

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	;DNA (5'-D(GPCPGPTP5IUPCPGPC)-3') ;
formula_weight	2530.5 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	B
atom_count	938

GCGTUCGC

Entity 2 polymer

description	;DNA (5'-D(GPCPGPAPAPCPGPC)-3') ;
formula_weight	2436.6 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	C

GCGAACGC

Entity 3 polymer

description	PROTEIN (CHROMOSOMAL PROTEIN SSO7D)
formula_weight	7164.4 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

ATVKFKYKGEEKEVDISKIKKVWRVGKMISFTYDEGGGKTGRGAVSEKDAPKELLQMLEKQKK

Source

organism	Sulfolobus acidocaldarius
ncbi_taxonomy_id	2285
host_organism	Escherichia coli

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	114

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6. Citations (1)

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length_a	46.870 Å
length_b	49.670 Å
length_c	37.650 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.600 Å
resolution_low	6.000 Å
r_factor	0.2110
r_work	0.2110
r_free	0.2880

fit	/app/data/saxs_computation/bf/1bf4/1bf4.fit
pddf	/app/data/pr_computation/bf/1bf4/1bf4.pr.out
profile	/app/data/saxs_computation/bf/1bf4/1bf4.dat

CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)