SAXSdb — 1bf5

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1. Structure Basics

entry_id	1bf5
deposition_date	1998-05-27
title	TYROSINE PHOSPHORYLATED STAT-1/DNA COMPLEX
keywords	COMPLEX (SH2 DOMAIN-DNA), SH2 DOMAIN, TRANSCRIPTION FACTOR, GENE REGULATION-DNA COMPLEX; GENE REGULATION/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	33.74 Å
rg_electron	33.89 Å
i0	98380000.00
molecular_weight	73239.0 kDa
excluded_volume	89228 Å³
envelope_volume	119690 Å³
shell_volume	32366 Å³
envelope_diameter	141.7 Å
shell_rg	36.60 Å
envelope_rg	34.55 Å
shape_rg	33.89 Å
total_rg	34.09 Å
total_atoms	5117
n_residues	580
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	133.8 Å
rg_real	34.12 Å
rg_real_error	1.75 Å
i0_real	9.8380e+07
i0_real_error	1.9180e+06
rg_reciprocal	33.88 Å
i0_reciprocal	98360000.0000
total_estimate	0.7505
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	34.2 Å
skewness	0.715
kurtosis	0.294
angular_range	— – 0.2350 Å⁻¹
current_alpha	0.0000
highest_alpha	9097000.0000
n_real_points	48
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.471; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.388; Smooth: 0.952

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	;DNA (5'-D(APCPAPGPTPTPTPCPCPCPGPTPAPAPAPTPG PC)-3') ;
formula_weight	5475.6 kDa
src_method	man
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	B
atom_count	5117

ACAGTTTCCCGTAAATGC

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21

Entity 2 polymer

description	;DNA (5'-D(TPGPCPAPTPTPTPAPCPGPGPGPAPAPAPCPT PG)-3') ;
formula_weight	5555.6 kDa
src_method	man
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	C

TGCATTTACGGGAAACTG

Entity 3 polymer

description	SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION 1-ALPHA/BETA
formula_weight	66657.3 kDa
mutation	PHOSPHORYLATED TYR 701
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

LDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWKRRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAEPRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIESILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVS

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

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6. Citations (1)

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length_a	76.600 Å
length_b	148.200 Å
length_c	181.100 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 2 2 21
int_tables_number	20
crystal_system	—

resolution	2.900 Å
resolution_low	30.000 Å
r_factor	0.2270
r_work	0.2270
r_free	0.2930

fit	/app/data/saxs_computation/bf/1bf5/1bf5.fit
pddf	/app/data/pr_computation/bf/1bf5/1bf5.pr.out
profile	/app/data/saxs_computation/bf/1bf5/1bf5.dat

TYROSINE PHOSPHORYLATED STAT-1/DNA COMPLEX

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)