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1bhm

RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA

Method: X-RAY DIFFRACTION D_max: 74.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1bhm
deposition_date	1995-07-12
title	RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA
keywords	HYDROLASE, COMPLEX (ENDONUCLEASE-DNA), NUCLEASE, HYDROLASE-DNA COMPLEX; HYDROLASE/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	22.88 Å
rg_electron	22.27 Å
i0	50350300.00
molecular_weight	52499.0 kDa
excluded_volume	64409 Å³
envelope_volume	73775 Å³
shell_volume	27223 Å³
envelope_diameter	79.8 Å
shell_rg	29.77 Å
envelope_rg	22.54 Å
shape_rg	22.29 Å
total_rg	23.03 Å
total_atoms	3671
n_residues	429
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	74.6 Å
rg_real	22.80 Å
rg_real_error	0.46 Å
i0_real	5.0350e+07
i0_real_error	6.2750e+05
rg_reciprocal	22.82 Å
i0_reciprocal	50350000.0000
total_estimate	0.8068
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	73.4 Å
skewness	0.299
kurtosis	-0.272
angular_range	— – 0.3450 Å⁻¹
current_alpha	0.0000
highest_alpha	11940000.0000
n_real_points	66
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.829; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	;DNA (5'-D(TPAPTPGPGPAPTPCPCPAPTPA)-3') ;
formula_weight	3661.4 kDa
src_method	syn
entity_count	2

Polymer

type	polydeoxyribonucleotide
strand_id	C,D
atom_count	3886

TATGGATCCATA

Entity 2 polymer

description	PROTEIN (BAMHI (E.C.3.1.21.4))
formula_weight	24602.3 kDa
ec_number	3.1.21.4
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B

MEVEKEFITDEAKELLSKDKLIQQAYNEVKTSICSPIWPATSKTFTINNTEKNCNGVVPIKELCYTLLEDTYNWYREKPLDILKLEKKKGGPIDVYKEFIENSELKRVGMEFETGNISSAHRSMNKLLLGLKHGEIDLAIILMPIKQLAYYLTDRVTNFEELEPYFELTEGQPFIFIGFNAEAYNSNVPLIPKGSDGMSKRSIKKWKDKVENK

Source

organism	Bacillus amyloliquefaciens
ncbi_taxonomy_id	1390

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	215

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6. Citations (1)

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length_a	108.800 Å
length_b	81.900 Å
length_c	68.800 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	2.200 Å
resolution_low	10.000 Å
r_factor	0.1890
r_work	—
r_free	—

fit	/app/data/saxs_computation/bh/1bhm/1bhm.fit
pddf	/app/data/pr_computation/bh/1bhm/1bhm.pr.out
profile	/app/data/saxs_computation/bh/1bhm/1bhm.dat

RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)