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1esg

RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.

Method: X-RAY DIFFRACTION D_max: 77.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1esg
deposition_date	2000-04-09
title	RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.
keywords	Non-specific DNA-protein complex., HYDROLASE-DNA COMPLEX; HYDROLASE/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	24.40 Å
rg_electron	23.68 Å
i0	49199700.00
molecular_weight	53331.0 kDa
excluded_volume	66317 Å³
envelope_volume	79415 Å³
shell_volume	28028 Å³
envelope_diameter	79.6 Å
shell_rg	30.78 Å
envelope_rg	23.66 Å
shape_rg	23.66 Å
total_rg	24.54 Å
total_atoms	3736
n_residues	437
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	77.5 Å
rg_real	24.33 Å
rg_real_error	0.38 Å
i0_real	4.9200e+07
i0_real_error	5.8370e+05
rg_reciprocal	24.35 Å
i0_reciprocal	49200000.0000
total_estimate	0.8979
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	29.5 Å
skewness	0.287
kurtosis	-0.376
angular_range	— – 0.3250 Å⁻¹
current_alpha	0.0000
highest_alpha	10100000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.898; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.975

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	;DNA (5'-D(TPGPGPAPTPTPCPA)-3') ;
formula_weight	2441.6 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	C
atom_count	4315

TGGATTCA

Entity 2 polymer

description	;DNA (5'-D(TPGPAPAPTPCPCPA)-3') ;
formula_weight	2410.6 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	D

TGAATCCA

Entity 3 polymer

description	TYPE II RESTRICTION ENZYME BAMHI
formula_weight	24602.3 kDa
ec_number	3.1.21.4
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B

MEVEKEFITDEAKELLSKDKLIQQAYNEVKTSICSPIWPATSKTFTINNTEKNCNGVVPIKELCYTLLEDTYNWYREKPLDILKLEKKKGGPIDVYKEFIENSELKRVGMEFETGNISSAHRSMNKLLLGLKHGEIDLAIILMPIKQLAYYLTDRVTNFEELEPYFELTEGQPFIFIGFNAEAYNSNVPLIPKGSDGMSKRSIKKWKDKVENK

Source

organism	Bacillus amyloliquefaciens
ncbi_taxonomy_id	1390
host_organism	Escherichia coli

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	579

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6. Citations (2)

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length_a	114.800 Å
length_b	91.100 Å
length_c	66.400 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.900 Å
resolution_low	10.000 Å
r_factor	—
r_work	0.1907
r_free	0.2302

fit	/app/data/saxs_computation/es/1esg/1esg.fit
pddf	/app/data/pr_computation/es/1esg/1esg.pr.out
profile	/app/data/saxs_computation/es/1esg/1esg.dat

RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)