← Back to search

1bj4

RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (HUMAN)

Method: X-RAY DIFFRACTION D_max: 83.0 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1bj4
deposition_date	1998-07-02
title	RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (HUMAN)
keywords	;TRANSFERASE, METABOLIC ROLE, PYRIDOXAL 5'-PHOSPHATE ;; TRANSFERASE
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	25.00 Å
rg_electron	24.06 Å
i0	44970600.00
molecular_weight	51639.0 kDa
excluded_volume	64506 Å³
envelope_volume	80483 Å³
shell_volume	27685 Å³
envelope_diameter	87.1 Å
shell_rg	31.39 Å
envelope_rg	24.55 Å
shape_rg	24.07 Å
total_rg	24.83 Å
total_atoms	3631
n_residues	470
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	83.0 Å
rg_real	24.97 Å
rg_real_error	0.57 Å
i0_real	4.4970e+07
i0_real_error	6.1420e+05
rg_reciprocal	24.98 Å
i0_reciprocal	44970000.0000
total_estimate	0.6845
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	29.0 Å
skewness	0.311
kurtosis	-0.300
angular_range	— – 0.3150 Å⁻¹
current_alpha	0.0000
highest_alpha	6824000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.866; Stabil: 1.000; Sysdev: 0.120; Positv: 1.000; Valcen: 0.982; Smooth: 0.953

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (3)

Entity 1 polymer

description	Serine hydroxymethyltransferase, cytosolic
formula_weight	51723.9 kDa
ec_number	2.1.2.1
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	3728

DADLWSSHDKMLAQPLKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELETLCQKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINYDQLEENARLFHPKLIIAGTSCYSRNLEYARLRKIADENGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVKSVDPKTGKEILYNLESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGL

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 non-polymer

description	PYRIDOXAL-5'-PHOSPHATE
formula_weight	247.1 kDa
src_method	syn
entity_count	1

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	97

▶

6. Citations (1)

▶

length_a	154.400 Å
length_b	154.400 Å
length_c	235.400 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	12

space_group	P 62 2 2
int_tables_number	180
crystal_system	—

resolution	2.650 Å
resolution_low	40.000 Å
r_factor	0.2100
r_work	0.2100
r_free	0.2260

fit	/app/data/saxs_computation/bj/1bj4/1bj4.fit
pddf	/app/data/pr_computation/bj/1bj4/1bj4.pr.out
profile	/app/data/saxs_computation/bj/1bj4/1bj4.dat

RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (HUMAN)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction