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1bmv

PROTEIN-RNA INTERACTIONS IN AN ICOSAHEDRAL VIRUS AT 3.0 ANGSTROMS RESOLUTION

Method: X-RAY DIFFRACTION D_max: 107.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1bmv
deposition_date	1989-10-09
title	PROTEIN-RNA INTERACTIONS IN AN ICOSAHEDRAL VIRUS AT 3.0 ANGSTROMS RESOLUTION
keywords	PROTEIN-RNA COMPLEX, SINGLE STRAND, Icosahedral virus, Virus-RNA COMPLEX; Virus/RNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	28.24 Å
rg_electron	27.36 Å
i0	74024500.00
molecular_weight	65379.0 kDa
excluded_volume	80896 Å³
envelope_volume	104410 Å³
shell_volume	32214 Å³
envelope_diameter	111.7 Å
shell_rg	34.17 Å
envelope_rg	28.54 Å
shape_rg	27.36 Å
total_rg	28.02 Å
total_atoms	4570
n_residues	570
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	107.2 Å
rg_real	28.34 Å
rg_real_error	1.24 Å
i0_real	7.4020e+07
i0_real_error	1.2500e+06
rg_reciprocal	28.31 Å
i0_reciprocal	74020000.0000
total_estimate	0.8120
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	30.6 Å
skewness	0.492
kurtosis	-0.027
angular_range	— – 0.2800 Å⁻¹
current_alpha	0.0000
highest_alpha	13790000.0000
n_real_points	57
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.588; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.790; Smooth: 0.999

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	;RNA (5'-R(GPGPUPCPAPAPAPAPUPGP*C)-3') ;
formula_weight	3530.2 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	M
atom_count	4613

GGUCAAAAUGC

Entity 2 polymer

description	PROTEIN (ICOSAHEDRAL VIRUS - A DOMAIN)
formula_weight	21914.5 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	1

SISQQTVWNQMATVRTPLNFDSSKQSFCQFSVDLLGGGISVDKTGDWITLVQNSPISNLLRVAAWKKGCLMVKVVMSGNAAVKRSDWASLVQVFLTNSNSTEHFDACRWTKSEPHSWELIFPIEVCGPNNGFEMWSSEWANQTSWHLSFLVDNPKQSTTFDVLLGISQNFEIAGNTLMPAFSVPQANARSSENAESSA

Source

organism	Bean pod mottle virus
ncbi_taxonomy_id	12260
host_organism	Escherichia coli

Entity 3 polymer

description	PROTEIN (ICOSAHEDRAL VIRUS - B AND C DOMAIN)
formula_weight	41223.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	2

METNLFKLSLDDVETPKGSMLDLKISQSKIALPKNTVGGTILRSDLLANFLTEGNFRASVDLQRTHRIKGMIKMVATVGIPENTGIALACAMNSSIRGRASSDIYTICSQDCELWNPACTKAMTMSFNPNPCSDAWSLEFLKRTGFHCDIICVTGWTATPMQDVQVTIDWFISSQECVPRTYCVLNPQNPFVLNRWMGKLTFPQGTSRSVKRMPLSIGGGAGAKSAILMNMPNAVLSMWRYFVGDLVFEVSKMTSPYIKCTVSFFIAFGNLADDTINFEAFPHKLVQFGEIQEKVVLKFSQEEFLTAWSTQVRPATTLLADGCPYLYAMVHDSSVSTIPGDFVIGVKLTIIENMCAYGLNPGISGSRLLGTIPQ

Source

organism	Bean pod mottle virus
ncbi_taxonomy_id	12260
host_organism	Escherichia coli

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6. Citations (1)

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length_a	311.200 Å
length_b	284.200 Å
length_c	350.500 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	120

space_group	P 2 21 21
int_tables_number	18
crystal_system	—

resolution	3.000 Å
resolution_low	7.000 Å
r_factor	0.3300
r_work	—
r_free	—

fit	/app/data/saxs_computation/bm/1bmv/1bmv.fit
pddf	/app/data/pr_computation/bm/1bmv/1bmv.pr.out
profile	/app/data/saxs_computation/bm/1bmv/1bmv.dat

PROTEIN-RNA INTERACTIONS IN AN ICOSAHEDRAL VIRUS AT 3.0 ANGSTROMS RESOLUTION

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)