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1c8x

Endo-Beta-N-Acetylglucosaminidase H, D130E Mutant

Method: X-RAY DIFFRACTION D_max: 61.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1c8x
deposition_date	1999-07-30
title	Endo-Beta-N-Acetylglucosaminidase H, D130E Mutant
keywords	(ALPHA/BETA)8-BARREL, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	19.19 Å
rg_electron	17.86 Å
i0	15032800.00
molecular_weight	28605.0 kDa
excluded_volume	35448 Å³
envelope_volume	38812 Å³
shell_volume	18218 Å³
envelope_diameter	62.3 Å
shell_rg	24.09 Å
envelope_rg	18.06 Å
shape_rg	17.85 Å
total_rg	18.78 Å
total_atoms	2022
n_residues	265
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	61.1 Å
rg_real	19.10 Å
rg_real_error	0.29 Å
i0_real	1.5030e+07
i0_real_error	1.6570e+05
rg_reciprocal	19.11 Å
i0_reciprocal	15030000.0000
total_estimate	0.8986
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	23.8 Å
skewness	0.216
kurtosis	-0.397
angular_range	— – 0.4150 Å⁻¹
current_alpha	0.0000
highest_alpha	2836000.0000
n_real_points	73
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.895; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.994

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	ENDO-BETA-N-ACETYLGLUCOSAMINIDASE H
formula_weight	28539.4 kDa
ec_number	3.2.1.96
mutation	D130E
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2174

KQGPTSVAYVEVNNNSMLNVGKYTLADGGGNAFDVAVIFAANINYDTGTKTAYLHFNENVQRVLDNAVTQIRPLQQQGIKVLLSVLGNHQGAGFANFPSQQAASAFAKQLSDAVAKYGLDGVDFEDEYAEYGNNGTAQPNDSSFVHLVTALRANMPDKIISLYNIGPAASRLSYGGVDVSDKFDYAWNPYYGTWQVPGIALPKAQLSPAAVEIGRTSRSTVADLARRTVDEGYGVYLTYNLDGGDRTADVSAFTRELYGSEAVRT

Source

organism	Streptomyces plicatus
ncbi_taxonomy_id	1922
host_organism	Escherichia coli

Entity 2 non-polymer

description	PHOSPHATE ION
formula_weight	95.0 kDa
src_method	syn
entity_count	1

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	152

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6. Citations (1)

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length_a	44.200 Å
length_b	55.200 Å
length_c	46.200 Å
angle_alpha	90.00 °
angle_beta	103.80 °
angle_gamma	90.00 °
z_pdb	2

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	2.000 Å
resolution_low	10.000 Å
r_factor	0.1820
r_work	0.1820
r_free	—

fit	/app/data/saxs_computation/c8/1c8x/1c8x.fit
pddf	/app/data/pr_computation/c8/1c8x/1c8x.pr.out
profile	/app/data/saxs_computation/c8/1c8x/1c8x.dat

Endo-Beta-N-Acetylglucosaminidase H, D130E Mutant

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction