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1ch0

RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT

Method: X-RAY DIFFRACTION D_max: 86.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ch0
deposition_date	1999-03-30
title	RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT
keywords	RIBONUCLEASE, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	25.67 Å
rg_electron	25.29 Å
i0	24657700.00
molecular_weight	34309.0 kDa
excluded_volume	41142 Å³
envelope_volume	51958 Å³
shell_volume	19379 Å³
envelope_diameter	88.0 Å
shell_rg	29.69 Å
envelope_rg	25.36 Å
shape_rg	25.24 Å
total_rg	25.94 Å
total_atoms	2408
n_residues	312
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	86.0 Å
rg_real	25.99 Å
rg_real_error	0.84 Å
i0_real	2.4660e+07
i0_real_error	3.5460e+05
rg_reciprocal	25.89 Å
i0_reciprocal	24660000.0000
total_estimate	0.8045
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	22.2 Å
skewness	0.596
kurtosis	-0.392
angular_range	— – 0.3100 Å⁻¹
current_alpha	0.0000
highest_alpha	8046000.0000
n_real_points	63
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.633; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.622; Smooth: 0.939

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	PROTEIN (RIBONUCLEASE T1)
formula_weight	11143.8 kDa
ec_number	3.1.27.3
mutation	Q25K, K41E, Y42F, N43R, Y45W, E46Q
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	A,B,C
atom_count	2552

ACDYTCGSNCYSSSDVSTAQAAGYKLHEDGETVGSNSYPHEFRNWQGFDFSVSSPYYEWPILSSGDVYSGGSPGADRVVFNENNQLAGVITHTGASGNNFVECT

Source

organism	Aspergillus oryzae
ncbi_taxonomy_id	5062
host_organism	Escherichia coli

Entity 2 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	4

Entity 3 non-polymer

description	GUANOSINE-2'-MONOPHOSPHATE
formula_weight	363.2 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	144

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6. Citations (1)

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length_a	39.820 Å
length_b	48.850 Å
length_c	158.770 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	12

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	2.300 Å
resolution_low	35.600 Å
r_factor	—
r_work	0.1980
r_free	0.2410

fit	/app/data/saxs_computation/ch/1ch0/1ch0.fit
pddf	/app/data/pr_computation/ch/1ch0/1ch0.pr.out
profile	/app/data/saxs_computation/ch/1ch0/1ch0.dat