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1cma

MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE

Method: X-RAY DIFFRACTION D_max: 61.9 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cma
deposition_date	1992-08-24
title	MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE
keywords	PROTEIN-DNA COMPLEX, DOUBLE HELIX, TRANSCRIPTION-DNA COMPLEX; TRANSCRIPTION/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	20.27 Å
rg_electron	18.98 Å
i0	21282000.00
molecular_weight	30628.0 kDa
excluded_volume	36433 Å³
envelope_volume	44527 Å³
shell_volume	19725 Å³
envelope_diameter	61.0 Å
shell_rg	25.16 Å
envelope_rg	19.15 Å
shape_rg	18.94 Å
total_rg	19.87 Å
total_atoms	2130
n_residues	227
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	61.9 Å
rg_real	20.17 Å
rg_real_error	0.28 Å
i0_real	2.1280e+07
i0_real_error	2.6520e+05
rg_reciprocal	20.19 Å
i0_reciprocal	21280000.0000
total_estimate	0.9130
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	25.6 Å
skewness	0.142
kurtosis	-0.537
angular_range	— – 0.3900 Å⁻¹
current_alpha	0.0000
highest_alpha	2565000.0000
n_real_points	71
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.958; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.995; Smooth: 0.995

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	;DNA (5'-D(TPTPAPGPAPCPGPTPCPT)-3') ;
formula_weight	3035.0 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	C
atom_count	2166

TTAGACGTCT

Entity 2 polymer

description	;DNA (5'-D(APGPAPCPGPTPCPTP*A)-3') ;
formula_weight	2739.8 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	D

AGACGTCTA

Entity 3 polymer

description	PROTEIN (MET REPRESSOR)
formula_weight	12027.6 kDa
src_method	nat
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B

AEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRRQVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPETWEY

Entity 4 non-polymer

description	S-ADENOSYLMETHIONINE
formula_weight	398.4 kDa
src_method	syn
entity_count	2

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	36

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6. Citations (2)

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length_a	121.350 Å
length_b	121.350 Å
length_c	84.810 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	24

space_group	P 62 2 2
int_tables_number	180
crystal_system	—

resolution	2.800 Å
resolution_low	10.000 Å
r_factor	0.2200
r_work	—
r_free	—

fit	/app/data/saxs_computation/cm/1cma/1cma.fit
pddf	/app/data/pr_computation/cm/1cma/1cma.pr.out
profile	/app/data/saxs_computation/cm/1cma/1cma.dat

MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Entity 4 non-polymer

Entity 5 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)