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1dux

ELK-1/DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION

Method: X-RAY DIFFRACTION D_max: 80.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1dux
deposition_date	2000-01-19
title	ELK-1/DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION
keywords	ETS-DOMAIN, DNA-BINDING DOMAIN, WINGED HELIX-TURN-HELIX, DNA-BINDING SPECIFICITY, Transcription-DNA COMPLEX; Transcription/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	24.66 Å
rg_electron	22.85 Å
i0	35172700.00
molecular_weight	36423.0 kDa
excluded_volume	41489 Å³
envelope_volume	53103 Å³
shell_volume	20689 Å³
envelope_diameter	82.0 Å
shell_rg	28.40 Å
envelope_rg	22.71 Å
shape_rg	22.72 Å
total_rg	23.70 Å
total_atoms	2512
n_residues	224
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	80.7 Å
rg_real	24.82 Å
rg_real_error	0.63 Å
i0_real	3.5170e+07
i0_real_error	5.7160e+05
rg_reciprocal	24.79 Å
i0_reciprocal	35170000.0000
total_estimate	0.8785
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	24.4 Å
skewness	0.437
kurtosis	-0.432
angular_range	— – 0.3200 Å⁻¹
current_alpha	0.0000
highest_alpha	2914000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.893; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.830; Smooth: 0.909

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	;DNA (5'-D(TPGPAPCPCPGPGPAPAPGPTPGP*T)-3') ;
formula_weight	4031.6 kDa
src_method	syn
entity_count	2

Polymer

type	polydeoxyribonucleotide
strand_id	A,D
atom_count	2775

TGACCGGAAGTGT

Entity 2 polymer

description	;DNA (5'-D(APCPAPCPTPTPCPCPGPGPTPCP*A)-3') ;
formula_weight	3911.6 kDa
src_method	syn
entity_count	2

Polymer

type	polydeoxyribonucleotide
strand_id	B,E

ACACTTCCGGTCA

Entity 3 polymer

description	ETS-DOMAIN PROTEIN ELK-1
formula_weight	11067.7 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,F

MDPSVTLWQFLLQLLREQGNGHIISWTSRDGGEFKLVDAEEVARLWGLRKNKTNMNYDKLSRALRYYYDKNIIRKVSGQKFVYKFVSYPEVAGC

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	225

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6. Citations (1)

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length_a	33.245 Å
length_b	140.523 Å
length_c	38.620 Å
angle_alpha	90.00 °
angle_beta	115.52 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	2.100 Å
resolution_low	50.000 Å
r_factor	—
r_work	0.2020
r_free	0.2360

fit	/app/data/saxs_computation/du/1dux/1dux.fit
pddf	/app/data/pr_computation/du/1dux/1dux.pr.out
profile	/app/data/saxs_computation/du/1dux/1dux.dat

ELK-1/DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)