1dw9

Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site

Method: X-RAY DIFFRACTION

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

CYANATE LYASE

ESCHERICHIA COLI

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	蛋白同源多聚体	同源多聚体	蛋白 10	CHLORIDE ION × 10 SULFATE ION × 23 water × 25	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	CYNS_ECOLI
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–156； UniProt 1–156 作者链 B； PDB构建体 1–156； UniProt 1–156 作者链 C； PDB构建体 1–156； UniProt 1–156 作者链 D； PDB构建体 1–156； UniProt 1–156 作者链 E； PDB构建体 1–156； UniProt 1–156 作者链 F； PDB构建体 1–156； UniProt 1–156 作者链 G； PDB构建体 1–156； UniProt 1–156 作者链 H； PDB构建体 1–156； UniProt 1–156 作者链 I； PDB构建体 1–156； UniProt 1–156 作者链 J； PDB构建体 1–156； UniProt 1–156

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	1dw9
沉积日期 deposition_date	1999-12-03
结构标题 title	Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site
关键词 keywords	LYASE, CYANATE DEGRADATION, STRUCTURAL GENOMICS, PSI, PROTEIN STRUCTURE INITIATIVE, MIDWEST CENTER FOR STRUCTURAL GENOMICS, MCSG; LYASE
实验方法 method	X-RAY DIFFRACTION

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3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

1dw9__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

1dw9__assembly_1__model_1 | I(q)

100 个绘图点；横轴和纵轴均采用对数尺度。

1dw9__assembly_1__model_1 | P(r) · Pending

P(r) 将按相同 assembly/model 分别计算和展示。

Rg（Guinier）	33.25 Å
Rg（电子密度）	32.08 Å
总 Rg	32.81 Å
原子数	12095
残基数	1520
排除体积	218860 Å³
最大 q	0.500 Å⁻¹

Assembly	Model	结构单元	聚集说明	状态	CRYSOL	操作
1	1	`1dw9__assembly_1__model_1`	decameric （10）	成功	4.1.3-1-20251215 (887e7ef)	查看下载

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

描述 description	CYANATE LYASE
分子量 formula_weight	17255.4 kDa
EC 编号 ec_number	4.3.99.1
来源方法 src_method	man
拷贝数 entity_count	10

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F,G,H,I,J
原子数 atom_count	14178

MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVTAALLGQQALPADAARLVGAKLDLDEDSILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQVYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF

来源 Source

来源生物 organism	ESCHERICHIA COLI
NCBI 分类编号 ncbi_taxonomy_id	562
宿主生物 host_organism	ESCHERICHIA COLI

实体 2 Entity 2 non-polymer

描述 description	CHLORIDE ION
分子量 formula_weight	35.5 kDa
来源方法 src_method	syn
拷贝数 entity_count	10

实体 3 Entity 3 non-polymer

描述 description	SULFATE ION
分子量 formula_weight	96.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	23

实体 4 Entity 4 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1865

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6. 折叠分类 (SCOP + CATH) 40 domains

SCOP 2.08 (20 domains)

结构域编号 domain_id	d1dw9a1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
家族 Family family	a.35.1.4 — Cyanase N-terminal domain

结构域编号 domain_id	d1dw9a2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.72 — Cyanase C-terminal domain
超家族 Superfamily superfamily	d.72.1 — Cyanase C-terminal domain
家族 Family family	d.72.1.1 — Cyanase C-terminal domain

结构域编号 domain_id	d1dw9b1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
家族 Family family	a.35.1.4 — Cyanase N-terminal domain

结构域编号 domain_id	d1dw9b2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.72 — Cyanase C-terminal domain
超家族 Superfamily superfamily	d.72.1 — Cyanase C-terminal domain
家族 Family family	d.72.1.1 — Cyanase C-terminal domain

结构域编号 domain_id	d1dw9c1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
家族 Family family	a.35.1.4 — Cyanase N-terminal domain

结构域编号 domain_id	d1dw9c2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.72 — Cyanase C-terminal domain
超家族 Superfamily superfamily	d.72.1 — Cyanase C-terminal domain
家族 Family family	d.72.1.1 — Cyanase C-terminal domain

结构域编号 domain_id	d1dw9d1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
家族 Family family	a.35.1.4 — Cyanase N-terminal domain

结构域编号 domain_id	d1dw9d2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.72 — Cyanase C-terminal domain
超家族 Superfamily superfamily	d.72.1 — Cyanase C-terminal domain
家族 Family family	d.72.1.1 — Cyanase C-terminal domain

结构域编号 domain_id	d1dw9e1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
家族 Family family	a.35.1.4 — Cyanase N-terminal domain

结构域编号 domain_id	d1dw9e2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.72 — Cyanase C-terminal domain
超家族 Superfamily superfamily	d.72.1 — Cyanase C-terminal domain
家族 Family family	d.72.1.1 — Cyanase C-terminal domain

结构域编号 domain_id	d1dw9f1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
家族 Family family	a.35.1.4 — Cyanase N-terminal domain

结构域编号 domain_id	d1dw9f2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.72 — Cyanase C-terminal domain
超家族 Superfamily superfamily	d.72.1 — Cyanase C-terminal domain
家族 Family family	d.72.1.1 — Cyanase C-terminal domain

结构域编号 domain_id	d1dw9g1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
家族 Family family	a.35.1.4 — Cyanase N-terminal domain

结构域编号 domain_id	d1dw9g2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.72 — Cyanase C-terminal domain
超家族 Superfamily superfamily	d.72.1 — Cyanase C-terminal domain
家族 Family family	d.72.1.1 — Cyanase C-terminal domain

结构域编号 domain_id	d1dw9h1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
家族 Family family	a.35.1.4 — Cyanase N-terminal domain

结构域编号 domain_id	d1dw9h2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.72 — Cyanase C-terminal domain
超家族 Superfamily superfamily	d.72.1 — Cyanase C-terminal domain
家族 Family family	d.72.1.1 — Cyanase C-terminal domain

结构域编号 domain_id	d1dw9i1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
家族 Family family	a.35.1.4 — Cyanase N-terminal domain

结构域编号 domain_id	d1dw9i2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.72 — Cyanase C-terminal domain
超家族 Superfamily superfamily	d.72.1 — Cyanase C-terminal domain
家族 Family family	d.72.1.1 — Cyanase C-terminal domain

结构域编号 domain_id	d1dw9j1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.35 — lambda repressor-like DNA-binding domains
超家族 Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
家族 Family family	a.35.1.4 — Cyanase N-terminal domain

结构域编号 domain_id	d1dw9j2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.72 — Cyanase C-terminal domain
超家族 Superfamily superfamily	d.72.1 — Cyanase C-terminal domain
家族 Family family	d.72.1.1 — Cyanase C-terminal domain

CATH v4.4 (20 domains)

结构域编号 domain_id	1dw9A01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily	40 — lambda repressor-like DNA-binding domains

结构域编号 domain_id	1dw9A02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1160 — Cyanate Lyase; Chain: A, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cyanate lyase, C-terminal domain

结构域编号 domain_id	1dw9B01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily	40 — lambda repressor-like DNA-binding domains

结构域编号 domain_id	1dw9B02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1160 — Cyanate Lyase; Chain: A, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cyanate lyase, C-terminal domain

结构域编号 domain_id	1dw9C01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily	40 — lambda repressor-like DNA-binding domains

结构域编号 domain_id	1dw9C02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1160 — Cyanate Lyase; Chain: A, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cyanate lyase, C-terminal domain

结构域编号 domain_id	1dw9D01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily	40 — lambda repressor-like DNA-binding domains

结构域编号 domain_id	1dw9D02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1160 — Cyanate Lyase; Chain: A, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cyanate lyase, C-terminal domain

结构域编号 domain_id	1dw9E01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily	40 — lambda repressor-like DNA-binding domains

结构域编号 domain_id	1dw9E02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1160 — Cyanate Lyase; Chain: A, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cyanate lyase, C-terminal domain

结构域编号 domain_id	1dw9F01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily	40 — lambda repressor-like DNA-binding domains

结构域编号 domain_id	1dw9F02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1160 — Cyanate Lyase; Chain: A, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cyanate lyase, C-terminal domain

结构域编号 domain_id	1dw9G01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily	40 — lambda repressor-like DNA-binding domains

结构域编号 domain_id	1dw9G02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1160 — Cyanate Lyase; Chain: A, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cyanate lyase, C-terminal domain

结构域编号 domain_id	1dw9H01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily	40 — lambda repressor-like DNA-binding domains

结构域编号 domain_id	1dw9H02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1160 — Cyanate Lyase; Chain: A, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cyanate lyase, C-terminal domain

结构域编号 domain_id	1dw9I01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily	40 — lambda repressor-like DNA-binding domains

结构域编号 domain_id	1dw9I02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1160 — Cyanate Lyase; Chain: A, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cyanate lyase, C-terminal domain

结构域编号 domain_id	1dw9J01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	260 — 434 Repressor (Amino-terminal Domain)
同源超家族 H-superfamily homologous superfamily	40 — lambda repressor-like DNA-binding domains

结构域编号 domain_id	1dw9J02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	1160 — Cyanate Lyase; Chain: A, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cyanate lyase, C-terminal domain

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晶胞边长 a length_a	76.340 Å
晶胞边长 b length_b	81.030 Å
晶胞边长 c length_c	82.300 Å
晶胞夹角 α angle_alpha	70.30 °
晶胞夹角 β angle_beta	72.20 °
晶胞夹角 γ angle_gamma	66.40 °
晶胞分子数 Z z_pdb	10

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	1.650 Å
最低分辨率 resolution_low	20.000 Å
R 因子 r_factor	—
R_work r_work	0.1500
R_free r_free	0.1890

Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site

1. 蛋白身份与相关结构 Protein Identity & Related Structures

CYANATE LYASE

当前结构中的状态

数据库中的同蛋白其他状态

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1dw9__assembly_1__model_1

1dw9__assembly_1__model_1 | I(q)

1dw9__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 non-polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 water

6. 折叠分类 (SCOP + CATH) 40 domains

SCOP 2.08 (20 domains)

CATH v4.4 (20 domains)

7. 引用文献 (5)

Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site

1. 蛋白身份与相关结构 Protein Identity & Related Structures

CYANATE LYASE

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1dw9__assembly_1__model_1

1dw9__assembly_1__model_1 | I(q)

1dw9__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 non-polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 water

6. 折叠分类 (SCOP + CATH) 40 domains

SCOP 2.08 (20 domains)

CATH v4.4 (20 domains)

7. 引用文献 (5)