SAXSdb — 8k6h

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Cyanate hydratase

Escherichia coli K-12

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 10	SULFATE ION × 25 cyanic acid × 10 water × 10	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CYNS_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 5–160； UniProt 1–156 Author chain B； PDBConstruct 5–160； UniProt 1–156 Author chain C； PDBConstruct 5–160； UniProt 1–156 Author chain D； PDBConstruct 5–160； UniProt 1–156 Author chain E； PDBConstruct 5–160； UniProt 1–156 Author chain F； PDBConstruct 5–160； UniProt 1–156 Author chain G； PDBConstruct 5–160； UniProt 1–156 Author chain H； PDBConstruct 5–160； UniProt 1–156 Author chain I； PDBConstruct 5–160； UniProt 1–156 Author chain J； PDBConstruct 5–160； UniProt 1–156

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8k6h
Deposition date deposition_date	2023-07-25
Last revision last_revision	2023-12-13
Structure title title	Crystal structure of e.coli cyanase complex with cyanate
Keywords keywords	enzyme, substrate complex, LYASE; LYASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8k6h__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8k6h__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8k6h__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	33.26 Å
Rg (electron density)	32.13 Å
Total Rg	32.99 Å
Atom count	12125
Residues	1560
Excluded volume	218520 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8k6h__assembly_1__model_1`	decameric （10）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Description description	Cyanate hydratase
Molecular weight formula_weight	17481.3 kDa
EC 编号 ec_number	4.2.1.104
Source method src_method	man
Copy count entity_count	10

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G,H,I,J
Atom count atom_count	14201

GSHMMIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVTAALLGQQALPADAARLVGAKLDLDEDSILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQVYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF

Source Source

Source organism organism	Escherichia coli K-12
NCBI taxonomy ID ncbi_taxonomy_id	83333
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 2 Entity 2 non-polymer

Description description	SULFATE ION
Molecular weight formula_weight	96.1 kDa
Source method src_method	syn
Copy count entity_count	25

Entity 3 Entity 3 non-polymer

Description description	cyanic acid
Molecular weight formula_weight	43.0 kDa
Source method src_method	syn
Copy count entity_count	10

Entity 4 Entity 4 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	1938

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Unit-cell length a length_a	78.136 Å
Unit-cell length b length_b	81.066 Å
Unit-cell length c length_c	81.065 Å
Unit-cell angle α angle_alpha	70.51 °
Unit-cell angle β angle_beta	76.37 °
Unit-cell angle γ angle_gamma	65.26 °
Molecules per unit cell Z z_pdb	10

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	1.500 Å
Low-resolution limit resolution_low	49.010 Å
R factor r_factor	0.1617
R_work r_work	0.1615
R_free r_free	0.1839

Crystal structure of e.coli cyanase complex with cyanate

1. Protein Identity and Related Structures Protein Identity & Related Structures

Cyanate hydratase

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8k6h__assembly_1__model_1

8k6h__assembly_1__model_1 | I(q)

8k6h__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 water

7. Citations (1)

Crystal structure of e.coli cyanase complex with cyanate

1. Protein Identity and Related Structures Protein Identity & Related Structures

Cyanate hydratase

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8k6h__assembly_1__model_1

8k6h__assembly_1__model_1 | I(q)

8k6h__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 water

7. Citations (1)