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1e14

;PHOTOSYNTHETIC REACTION CENTER MUTANT WITH PHE M197 REPLACED WITH ARG (CHAIN M, FM197R) AND GLY M203 REPLACED WITH ASP (CHAIN M, GM203D) ;

Method: X-RAY DIFFRACTION D_max: 91.7 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1e14
deposition_date	2000-04-18
title	;PHOTOSYNTHETIC REACTION CENTER MUTANT WITH PHE M197 REPLACED WITH ARG (CHAIN M, FM197R) AND GLY M203 REPLACED WITH ASP (CHAIN M, GM203D) ;
keywords	TRANSMEMBRANE, ELECTRON TRANSPORT, PHOTOSYNTHESIS; ELECTRON TRANSPORT
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	29.56 Å
rg_electron	28.18 Å
i0	121968000.00
molecular_weight	100500.0 kDa
excluded_volume	130930 Å³
envelope_volume	145700 Å³
shell_volume	41308 Å³
envelope_diameter	96.1 Å
shell_rg	36.97 Å
envelope_rg	28.40 Å
shape_rg	28.18 Å
total_rg	29.08 Å
total_atoms	7138
n_residues	825
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	91.7 Å
rg_real	29.44 Å
rg_real_error	0.47 Å
i0_real	1.2200e+08
i0_real_error	1.6830e+06
rg_reciprocal	29.49 Å
i0_reciprocal	122000000.0000
total_estimate	0.9089
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	35.6 Å
skewness	0.156
kurtosis	-0.560
angular_range	— – 0.2700 Å⁻¹
current_alpha	0.0000
highest_alpha	14410000.0000
n_real_points	55
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.954; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.953

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4. Crystallography & Experiment

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5. Entities & Polymer Info (11)

Entity 1 polymer

description	Reaction center protein H chain
formula_weight	28066.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	H
atom_count	7250

MVGVTAFGNFDLASLAIYSFWIFLAGLIYYLQTENMREGYPLENEDGTPAANQGPFPLPKPKTFILPHGRGTLTVPGPESEDRPIALARTAVSEGFPHAPTGDPMKDGVGPASWVARRDLPELDGHGHNKIKPMKAAAGFHVSAGKNPIGLPVRGCDLEIAGKVVDIWVDIPEQMARFLEVELKDGSTRLLPMQMVKVQSNRVHVNALSSDLFAGIPTIKSPTEVTLLEEDKICGYVAGGLMYAAPKRKSVVAAMLAEYA

Source

organism	Rhodobacter sphaeroides
ncbi_taxonomy_id	1063
host_organism	RHODOBACTER SPHAEROIDES

Entity 2 polymer

description	Reaction center protein L chain
formula_weight	31346.4 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	L

ALLSFERKYRVPGGTLVGGNLFDFWVGPFYVGFFGVATFFFAALGIILIAWSAVLQGTWNPQLISVYPPALEYGLGGAPLAKGGLWQIITICATGAFVSWALREVEICRKLGIGYHIPFAFAFAILAYLTLVLFRPVMMGAWGYAFPYGIWTHLDWVSNTGYTYGNFHYNPAHMIAISFFFTNALALALHGALVLSAANPEKGKEMRTPDHEDTFFRDLVGYSIGTLGIHRLGLLLSLSAVFFSALCMIITGTIWFDQWVDWWQWWVKLPWWANIPGGING

Source

organism	Rhodobacter sphaeroides
ncbi_taxonomy_id	1063
host_organism	RHODOBACTER SPHAEROIDES

Entity 3 polymer

description	Reaction center protein M chain
formula_weight	34466.6 kDa
mutation	YES
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	M

AEYQNIFSQVQVRGPADLGMTEDVNLANRSGVGPFSTLLGWFGNAQLGPIYLGSLGVLSLFSGLMWFFTIGIWFWYQAGWNPAVFLRDLFFFSLEPPAPEYGLSFAAPLKEGGLWLIASFFMFVAVWSWWGRTYLRAQALGMGKHTAWAFLSAIWLWMVLGFIRPILMGSWSEAVPYGIFSHLDWTNNFSLVHGNLRYNPFHDLSIAFLYGSALLFAMHGATILAVSRFGGERELEQIADRGTAAERAALFWRWTMGFNATMEGIHRWAIWMAVLVTLTGGIGILLSGTVVDNWYVWGQNHGMAPLN

Source

organism	Rhodobacter sphaeroides
ncbi_taxonomy_id	1063
host_organism	RHODOBACTER SPHAEROIDES

Entity 4 non-polymer

description	LAURYL DIMETHYLAMINE-N-OXIDE
formula_weight	229.4 kDa
src_method	syn
entity_count	3

Entity 5 non-polymer

description	BACTERIOCHLOROPHYLL A
formula_weight	911.5 kDa
src_method	syn
entity_count	4

Entity 6 non-polymer

description	BACTERIOPHEOPHYTIN A
formula_weight	889.2 kDa
src_method	syn
entity_count	2

Entity 7 non-polymer

description	UBIQUINONE-10
formula_weight	863.3 kDa
src_method	syn
entity_count	2

Entity 8 non-polymer

description	FE (III) ION
formula_weight	55.8 kDa
src_method	syn
entity_count	1

Entity 9 non-polymer

description	SPEROIDENONE
formula_weight	595.0 kDa
src_method	syn
entity_count	1

Entity 10 non-polymer

description	CARDIOLIPIN
formula_weight	1464.0 kDa
src_method	syn
entity_count	1

Entity 11 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	112

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6. Citations (3)

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length_a	140.000 Å
length_b	140.000 Å
length_c	184.600 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 31 2 1
int_tables_number	152
crystal_system	—

resolution	2.700 Å
resolution_low	30.000 Å
r_factor	—
r_work	0.2260
r_free	0.2680

fit	/app/data/saxs_computation/e1/1e14/1e14.fit
pddf	/app/data/pr_computation/e1/1e14/1e14.pr.out
profile	/app/data/saxs_computation/e1/1e14/1e14.dat

;PHOTOSYNTHETIC REACTION CENTER MUTANT WITH PHE M197 REPLACED WITH ARG (CHAIN M, FM197R) AND GLY M203 REPLACED WITH ASP (CHAIN M, GM203D) ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (11)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 non-polymer

Entity 7 non-polymer

Entity 8 non-polymer

Entity 9 non-polymer

Entity 10 non-polymer

Entity 11 water

6. Citations (3)

7. Files & Curves (10)

Files (7)

Curves (3)