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1e2v

N153Q mutant of cytochrome f from Chlamydomonas reinhardtii

Method: X-RAY DIFFRACTION D_max: 119.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1e2v
deposition_date	2000-05-29
title	N153Q mutant of cytochrome f from Chlamydomonas reinhardtii
keywords	ELECTRON TRANSPORT, INTERNAL WATER CHAIN, PHOTOSYNTHETIC FUNCTION IMPAIRED; ELECTRON TRANSPORT
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	34.27 Å
rg_electron	34.10 Å
i0	103661000.00
molecular_weight	83537.0 kDa
excluded_volume	105710 Å³
envelope_volume	145690 Å³
shell_volume	37668 Å³
envelope_diameter	128.8 Å
shell_rg	38.28 Å
envelope_rg	34.24 Å
shape_rg	34.07 Å
total_rg	34.55 Å
total_atoms	5892
n_residues	753
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	119.1 Å
rg_real	34.43 Å
rg_real_error	1.72 Å
i0_real	1.0370e+08
i0_real_error	1.9360e+06
rg_reciprocal	34.33 Å
i0_reciprocal	103700000.0000
total_estimate	0.8588
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	41.4 Å
skewness	0.511
kurtosis	-0.051
angular_range	— – 0.2300 Å⁻¹
current_alpha	0.0000
highest_alpha	7587000.0000
n_real_points	47
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.769; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.967; Smooth: 0.890

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	CYTOCHROME F
formula_weight	27196.0 kDa
mutation	YES
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	A,B,C
atom_count	7104

YPVFAQQNYANPREANGRIVCANCHLAQKAVEIEVPQAVLPDTVFEAVIELPYDKQVKQVLANGKKGDLNVGMVLILPEGFELAPPDRVPAEIKEKVGNLYYQPYSPEQKNILVVGPVPGKKYSEMVVPILSPDPAKNKNVSYLKYPIYFGGQRGRGQVYPDGKKSNNTIYNASAAGKIVAITALSEKKGGFEVSIEKANGEVVVDKIPAGPDLIVKEGQTVQADQPLTNNPNVGGFGQAETEIVLQNPAR

Source

organism	CHLAMYDOMONAS REINHARDTII
ncbi_taxonomy_id	3055
host_organism	ESCHERICHIA COLI

Entity 2 non-polymer

description	HEME C
formula_weight	618.5 kDa
src_method	syn
entity_count	3

Entity 3 non-polymer

description	ACETATE ION
formula_weight	59.0 kDa
src_method	syn
entity_count	3

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	1186

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6. Citations (1)

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length_a	76.120 Å
length_b	94.220 Å
length_c	121.080 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	12

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.850 Å
resolution_low	42.330 Å
r_factor	0.1850
r_work	0.1850
r_free	0.2190

fit	/app/data/saxs_computation/e2/1e2v/1e2v.fit
pddf	/app/data/pr_computation/e2/1e2v/1e2v.pr.out
profile	/app/data/saxs_computation/e2/1e2v/1e2v.dat

N153Q mutant of cytochrome f from Chlamydomonas reinhardtii

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction