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1e3o

Crystal structure of Oct-1 POU dimer bound to MORE

Method: X-RAY DIFFRACTION D_max: 62.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1e3o
deposition_date	2000-06-20
title	Crystal structure of Oct-1 POU dimer bound to MORE
keywords	TRANSCRIPTION, POU DOMAIN, DIMER, DNA BINDING; TRANSCRIPTION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	17.86 Å
rg_electron	17.61 Å
i0	11930600.00
molecular_weight	21542.0 kDa
excluded_volume	25105 Å³
envelope_volume	30720 Å³
shell_volume	15189 Å³
envelope_diameter	60.5 Å
shell_rg	23.01 Å
envelope_rg	17.83 Å
shape_rg	17.63 Å
total_rg	18.32 Å
total_atoms	1493
n_residues	154
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	62.1 Å
rg_real	17.86 Å
rg_real_error	0.45 Å
i0_real	1.1930e+07
i0_real_error	1.6570e+05
rg_reciprocal	17.86 Å
i0_reciprocal	11930000.0000
total_estimate	0.8601
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	21.0 Å
skewness	0.391
kurtosis	-0.169
angular_range	— – 0.4450 Å⁻¹
current_alpha	0.0000
highest_alpha	2336000.0000
n_real_points	75
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.736; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.974; Smooth: 0.997

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	5'-D(APTPGPCPAPTPGPAPGPGP*A)-3'
formula_weight	3422.3 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	A
atom_count	1631

ATGCATGAGGA

Entity 2 polymer

description	5'-D(TPCPCPTPCPAPTPGPCPAP*T)-3'
formula_weight	3284.2 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	B

TCCTCATGCAT

Entity 3 polymer

description	OCTAMER-BINDING TRANSCRIPTION FACTOR 1
formula_weight	18398.9 kDa
mutation	YES
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

EEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMSKLKPLLEKWLNDAEANLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFMENQKPTSEDITLIAEQLNMEKEVIRVWFSNRRQKEKRIN

Source

organism	Homo sapiens
common_name	Human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	138

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6. Citations (1)

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length_a	93.300 Å
length_b	52.400 Å
length_c	69.000 Å
angle_alpha	90.00 °
angle_beta	127.60 °
angle_gamma	90.00 °
z_pdb	4

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	1.900 Å
resolution_low	20.000 Å
r_factor	0.2200
r_work	0.2200
r_free	0.2420

fit	/app/data/saxs_computation/e3/1e3o/1e3o.fit
pddf	/app/data/pr_computation/e3/1e3o/1e3o.pr.out
profile	/app/data/saxs_computation/e3/1e3o/1e3o.dat

Crystal structure of Oct-1 POU dimer bound to MORE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)