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1e8o

Core of the Alu domain of the mammalian SRP

Method: X-RAY DIFFRACTION D_max: 92.9 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1e8o
deposition_date	2000-09-28
title	Core of the Alu domain of the mammalian SRP
keywords	;ALU RIBONUCLEOPROTEIN PARTICLE, PROTEIN RECOGNITION OF AN RNA U-TURN, TRANSLATIONAL CONTROL, ALU RNP ASSEMBLY AND TRANSPORT, ALU RETROPOSITION ;; ALU RIBONUCLEOPROTEIN PARTICLE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	27.66 Å
rg_electron	29.05 Å
i0	63611900.00
molecular_weight	50867.0 kDa
excluded_volume	58622 Å³
envelope_volume	81717 Å³
shell_volume	24747 Å³
envelope_diameter	99.8 Å
shell_rg	34.81 Å
envelope_rg	28.81 Å
shape_rg	29.07 Å
total_rg	29.48 Å
total_atoms	3486
n_residues	345
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	92.9 Å
rg_real	27.87 Å
rg_real_error	0.75 Å
i0_real	6.3610e+07
i0_real_error	9.1410e+05
rg_reciprocal	27.81 Å
i0_reciprocal	63610000.0000
total_estimate	0.8593
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	27.7 Å
skewness	0.490
kurtosis	-0.372
angular_range	— – 0.2850 Å⁻¹
current_alpha	0.0000
highest_alpha	4546000.0000
n_real_points	58
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.796; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.868; Smooth: 0.912

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN
formula_weight	9996.6 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,C
atom_count	3508

PQYQTWEEFSRAAEKLYLADPMKARVVLKYRHSDGNLCVKVTDDLVCLVYKTDQAQDVKKIEKFHSQLMRLMVAKEARNVTMETE

Source

organism	HOMO SAPIENS
common_name	HUMAN
ncbi_taxonomy_id	9606
host_organism	ESCHERICHIA COLI

Entity 2 polymer

description	SIGNAL RECOGNITION PARTICLE 14 KDA PROTEIN
formula_weight	12114.2 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	B,D

VLLESEQFLTELTRLFQKCRTSGSVYITLKKYDGRTKPIPKKGTVEGFEPADNKCLLRATDGKKKISTVVSSKEVNKFQMAYSNLLRANMDGLKKRDKKNKTKKTK

Source

organism	HOMO SAPIENS
common_name	HUMAN
ncbi_taxonomy_id	9606
host_organism	ESCHERICHIA COLI

Entity 3 polymer

description	7SL RNA
formula_weight	16277.6 kDa
mutation	YES
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	E

GGGCCGGGCGCGGUGGCGCGCGCCUGUAGUCCCAGCUACUCGGGAGGCUC

Entity 4 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	3

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	22

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6. Citations (1)

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length_a	57.448 Å
length_b	186.621 Å
length_c	189.824 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	C 2 2 21
int_tables_number	20
crystal_system	—

resolution	3.200 Å
resolution_low	47.460 Å
r_factor	0.2450
r_work	0.2450
r_free	0.2910

fit	/app/data/saxs_computation/e8/1e8o/1e8o.fit
pddf	/app/data/pr_computation/e8/1e8o/1e8o.pr.out
profile	/app/data/saxs_computation/e8/1e8o/1e8o.dat

Core of the Alu domain of the mammalian SRP

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Entity 4 non-polymer

Entity 5 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)