SAXSdb — 1ea0

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1. Structure Basics

entry_id	1ea0
deposition_date	2000-11-02
title	Alpha subunit of A. brasilense glutamate synthase
keywords	OXIDOREDUCTASE, IRON SULPHUR FLAVOPROTEIN; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	45.50 Å
rg_electron	45.03 Å
i0	1533240000.00
molecular_weight	320440.0 kDa
excluded_volume	399260 Å³
envelope_volume	490750 Å³
shell_volume	87692 Å³
envelope_diameter	160.5 Å
shell_rg	52.06 Å
envelope_rg	44.62 Å
shape_rg	45.01 Å
total_rg	45.32 Å
total_atoms	22478
n_residues	2904
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	147.9 Å
rg_real	45.43 Å
rg_real_error	1.30 Å
i0_real	1.5330e+09
i0_real_error	2.7840e+07
rg_reciprocal	45.50 Å
i0_reciprocal	1533000000.0000
total_estimate	0.6761
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	50.7 Å
skewness	0.279
kurtosis	-0.539
angular_range	— – 0.1750 Å⁻¹
current_alpha	0.0000
highest_alpha	356800000.0000
n_real_points	36
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.896; Stabil: 1.000; Sysdev: 0.062; Positv: 1.000; Valcen: 1.000; Smooth: 0.912

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	GLUTAMATE SYNTHASE [NADPH] LARGE CHAIN
formula_weight	162313.7 kDa
ec_number	1.4.1.13
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	22478

CGVGFIAAIDGKPRRSVVEKGIEALKAVWHRGAVDADGKTGDGAGIHVAVPQKFFKDHVKVIGHRAPDNKLAVGQVFLPRISLDAQEACRCIVETEILAFGYYIYGWRQVPINVDIIGEKANATRPEIEQIIVGNNKGVSDEQFELDLYIIRRRIEKAVKGEQINDFYICSLSARSIIYKGMFLAEQLTTFYPDLLDERFESDFAIYHQRYSTNTFPTWPLAQPFRMLAHNGEINTVKGNVNWMKAHETRMEHPAFGTHMQDLKPVIGVGLSDSGSLDTVFEVMVRAGRTAPMVKMMLVPQALTSSQTTPDNHKALIQYCNSVMEPWDGPAALAMTDGRWVVGGMDRNGLRPMRYTITTDGLIIGGSETGMVKIDETQVIEKGRLGPGEMIAVDLQSGKLYRDRELKDHLATLKPWDKWVQNTTHLDELVKTASLKGEPSDMDKAELRRRQQAFGLTMEDMELILHPMVEDGKEAIGSMGDDSPIAVLSDKYRGLHHFFRQNFSQVTNPPIDSLRERRVMSLKTRLGNLGNILDEDETQTRLLQLESPVLTTAEFRAMRDYMGDTAAEIDATFPVDGGPEALRDALRRIRQETEDAVRGGATHVILTDEAMGPARAAIPAILATGAVHTHLIRSNLRTFTSLNVRTAEGLDTHYFAVLIGVGATTVNAYLAQEAIAERHRRGLFGSMPLEKGMANYKKAIDDGLLKIMSKMGISVISSYRGGGNFEAIGLSRALVAEHFPAMVSRISGIGLNGIQKKVLEQHATAYNEEVVALPVGGFYRFRKSGDRHGWEGGVIHTLQQAVTNDSYTTFKKYSEQVNKRPPMQLRDLLELRSTKAPVPVDEVESITAIRKRFITPGMSMGALSPEAHGTLNVAMNRIGAKSDSGEGGEDPARFRPDKNGDNWNSAIKQVASGRFGVTAEYLNQCRELEIKVAQGAKPGEGGQLPGFKVTEMIARLRHSTPGVMLISPPPHHDIYSIEDLAQLIYDLKQINPDAKVTVKLVSRSGIGTIAAGVAKANADIILISGNSGGTGASPQTSIKFAGLPWEMGLSEVHQVLTLNRLRHRVRLRTDGGLKTGRDIVIAAMLGAEEFGIGTASLIAMGCIMVRQCHSNTCPVGVCVQDDKLRQKFVGTPEKVVNLFTFLAEEVREILAGLGFRSLNEVIGRTDLLHQVSRGAEHLDDLDLNPRLAQVDPGENARYCTLQGRNEVPDTLDARIVADARPLFEEGEKMQLAYNARNTQRAIGTRLSSMVTRKFGMFGLQPGHITIRLRGTAGQSLGAFAVQGIKLEVMGDANDYVGKGLSGGTIVVRPTTSSPLETNKNTIIGNTVLYGATAGKLFAAGQAGERFAVRNSGATVVVEGCGSNGCEYMTGGTAVILGRVGDNFAAGMTGGMAYVYDLDDSLPLYINDESVIFQRIEVGHYESQLKHLIEEHVTETQSRFAAEILNDWAREVTKFWQVVPKEMLNRLEVPVHLPKAISAE

Source

organism	AZOSPIRILLUM BRASILENSE
ncbi_taxonomy_id	192
host_organism	ESCHERICHIA COLI

Entity 2 non-polymer

description	S-DIOXYMETHIONINE
formula_weight	181.2 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	FLAVIN MONONUCLEOTIDE
formula_weight	456.3 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	2-OXOGLUTARIC ACID
formula_weight	146.1 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	FE3-S4 CLUSTER
formula_weight	295.8 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	233.612 Å
length_b	233.612 Å
length_c	305.089 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	12

space_group	P 31 2 1
int_tables_number	152
crystal_system	—

resolution	3.000 Å
resolution_low	20.000 Å
r_factor	0.2562
r_work	0.2559
r_free	0.2871

fit	/app/data/saxs_computation/ea/1ea0/1ea0.fit
pddf	/app/data/pr_computation/ea/1ea0/1ea0.pr.out
profile	/app/data/saxs_computation/ea/1ea0/1ea0.dat

Alpha subunit of A. brasilense glutamate synthase

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction